L-Homocysteine thiolactone hydrochloride
Based on 1 Customer Validation
L-Homocysteine thiolactone hydrochloride is an intramolecular thioester of Homocysteine. Homocysteine thiolactone hydrochloride prevents translational incorporation of homocysteine into proteins. L-Homocysteine thiolactone hydrochloride can induce elevated HHcy (hyperhomocysteinemia) in mice.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 31828-68-9
- Formula: C4H8ClNOS
- Molecular Weight:153.63
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
L-Homocysteine thiolactone hydrochloride (20 μM; for 24 h) shows significant increase in pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6[2].
L-Homocysteine thiolactone hydrochloride (100 μM; for 24 h) shows significantly higher ratios of nuclear/cytoplasmic NF-κB, indicating that Homocysteine (Hcy)-induced NF-κB activation and nuclear translocation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:U937 cells
-
Concentration:100 μM
-
Incubation Time:24 h
-
Result:Showed significantly higher ratios of nuclear/cytoplasmic NF-κB.
-
Cell Line:U937 cells
-
Concentration:20 μM
-
Incubation Time:24 h
-
Result:Showed significant increase in pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6.
Administration: 1 μL • intravitreally • 200 μM BL/6 mice (5 weeks old)
(2) L-Homocysteine thiolactone hydrochloride is dissolved in water and a working solution of 10x is prepared by diluting 1 μL of stock solution (200 mM) to 100 μL with PBS. Then by injecting 1 μL of this working solution, 200 μM vitreal concentration of Hcy-thiolactone is obtained.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:cbs+/- mice and normal wild type mice (C57BL6/J), 6-8 weeks of age[2]
-
Dosage:1 μL; 200 μM
-
Administration:Intravitreally
-
Result:Normal retinal flat-mount preparations from cbs+/+ mice showed quiescent ramified microglia, while cbs-/- and cbs+/- retinas demonstrated rod-like microglia devoid of branching processes, indicating microglia activation in HHcy mice.
Chemical Information
-
CAS No. 31828-68-9
-
Appearance Solid
-
Molecular Weight 153.63
-
Formula C4H8ClNOS
-
Color White to off-white
-
SMILES
N[C@@H](CCS1)C1=O.Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 200 mg/mL (1301.83 mM)
DMSO : 116.67 mg/mL (759.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (650.91 mM); Clear solution; Need ultrasonic
Purity & Documentation
-
Data Sheet (281 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Jakubowski H, et al. Homocysteine thiolactone: metabolic origin and protein homocysteinylation in humans. J Nutr. 2000 Feb;130(2S Suppl):377S-381S. [Content Brief]
[2]. Nehal M Elsherbiny, et al. Homocysteine Induces Inflammation in Retina and Brain. Biomolecules. 2020 Mar 3;10(3):393. [Content Brief]
[3]. Ahmed S Ibrahim, et al. Hyperhomocysteinemia disrupts retinal pigment epithelial structure and function with features of age-related macular degeneration. Oncotarget. 2016 Feb 23;7(8):8532-45. [Content Brief]
[4]. Olivera Stanojlović, et al. Two types of seizures in homocysteine thiolactone-treated adult rats, behavioral and electroencephalographic study. Cell Mol Neurobiol. 2009 May;29(3):329-39. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 6.5091 mL | 32.5457 mL | 65.0915 mL | 162.7286 mL |
| 5 mM | 1.3018 mL | 6.5091 mL | 13.0183 mL | 32.5457 mL | |
| 10 mM | 0.6509 mL | 3.2546 mL | 6.5091 mL | 16.2729 mL | |
| 15 mM | 0.4339 mL | 2.1697 mL | 4.3394 mL | 10.8486 mL | |
| 20 mM | 0.3255 mL | 1.6273 mL | 3.2546 mL | 8.1364 mL | |
| 25 mM | 0.2604 mL | 1.3018 mL | 2.6037 mL | 6.5091 mL | |
| 30 mM | 0.2170 mL | 1.0849 mL | 2.1697 mL | 5.4243 mL | |
| 40 mM | 0.1627 mL | 0.8136 mL | 1.6273 mL | 4.0682 mL | |
| 50 mM | 0.1302 mL | 0.6509 mL | 1.3018 mL | 3.2546 mL | |
| 60 mM | 0.1085 mL | 0.5424 mL | 1.0849 mL | 2.7121 mL | |
| 80 mM | 0.0814 mL | 0.4068 mL | 0.8136 mL | 2.0341 mL | |
| 100 mM | 0.0651 mL | 0.3255 mL | 0.6509 mL | 1.6273 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Homocysteine thiolactone hydrochloride Related Classifications
HY-101404A Related Classifications
Powered by Bioz