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L-Homocysteine thiolactone hydrochloride 

Cat. No.: HY-101404A Purity: 99.32%
COA Handling Instructions

L-Homocysteine thiolactone hydrochloride is an intramolecular thioester of Homocysteine. Homocysteine thiolactone hydrochloride prevents translational incorporation of homocysteine into proteins. L-Homocysteine thiolactone hydrochloride can induce elevated HHcy (hyperhomocysteinemia) in mice.

For research use only. We do not sell to patients.

L-Homocysteine thiolactone hydrochloride Chemical Structure

L-Homocysteine thiolactone hydrochloride Chemical Structure

CAS No. : 31828-68-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
USD 33 In-stock
Solution
10 mM * 1 mL in Water USD 33 In-stock
Solid
10 mg USD 30 In-stock
25 mg USD 60 In-stock
50 mg USD 98 In-stock
100 mg USD 156 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of L-Homocysteine thiolactone hydrochloride:

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  • Biological Activity

  • Purity & Documentation

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Description

L-Homocysteine thiolactone hydrochloride is an intramolecular thioester of Homocysteine. Homocysteine thiolactone hydrochloride prevents translational incorporation of homocysteine into proteins. L-Homocysteine thiolactone hydrochloride can induce elevated HHcy (hyperhomocysteinemia) in mice[1][2][3].

In Vitro

L-Homocysteine thiolactone hydrochloride (20 μM; for 24 h) shows significant increase in pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6[2].
L-Homocysteine thiolactone hydrochloride (100 μM; for 24 h) shows significantly higher ratios of nuclear/cytoplasmic NF-κB, indicating that Homocysteine (Hcy)-induced NF-κB activation and nuclear translocation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: U937 cells
Concentration: 100 μM
Incubation Time: 24 h
Result: Showed significantly higher ratios of nuclear/cytoplasmic NF-κB.

RT-PCR[2]

Cell Line: U937 cells
Concentration: 20 μM
Incubation Time: 24 h
Result: Showed significant increase in pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6.
In Vivo

L-Homocysteine thiolactone hydrochloride (1 μL; 200 μM; Intravitreally) induces elevated HHcy (hyperhomocysteinemia)[2]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: cbs+/- mice and normal wild type mice (C57BL6/J), 6-8 weeks of age[2]
Dosage: 1 μL; 200 μM
Administration: Intravitreally
Result: Normal retinal flat-mount preparations from cbs+/+ mice showed quiescent ramified microglia, while cbs-/- and cbs+/- retinas demonstrated rod-like microglia devoid of branching processes, indicating microglia activation in HHcy mice.
Molecular Weight

153.63

Formula

C4H8ClNOS

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCS1)C1=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 23 mg/mL (149.71 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5091 mL 32.5457 mL 65.0915 mL
5 mM 1.3018 mL 6.5091 mL 13.0183 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (650.91 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 6.5091 mL 32.5457 mL 65.0915 mL 162.7286 mL
5 mM 1.3018 mL 6.5091 mL 13.0183 mL 32.5457 mL
10 mM 0.6509 mL 3.2546 mL 6.5091 mL 16.2729 mL
15 mM 0.4339 mL 2.1697 mL 4.3394 mL 10.8486 mL
20 mM 0.3255 mL 1.6273 mL 3.2546 mL 8.1364 mL
25 mM 0.2604 mL 1.3018 mL 2.6037 mL 6.5091 mL
30 mM 0.2170 mL 1.0849 mL 2.1697 mL 5.4243 mL
40 mM 0.1627 mL 0.8136 mL 1.6273 mL 4.0682 mL
50 mM 0.1302 mL 0.6509 mL 1.3018 mL 3.2546 mL
60 mM 0.1085 mL 0.5424 mL 1.0849 mL 2.7121 mL
80 mM 0.0814 mL 0.4068 mL 0.8136 mL 2.0341 mL
100 mM 0.0651 mL 0.3255 mL 0.6509 mL 1.6273 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Homocysteine thiolactone hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
L-Homocysteine thiolactone hydrochloride
Cat. No.:
HY-101404A
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