1. GPCR/G Protein
  2. Orexin Receptor (OX Receptor)

Orexin B, rat, mouse (Synonyms: Rat orexin B; Orexin B (mouse))

Cat. No.: HY-P1349 Purity: 95.54%
Handling Instructions

Orexin B, rat, mouse is an endogenous agonist at Orexin receptor with Kis of 420 and 36 nM for OX1 and OX2, respectively. Sequence: Arg-Pro-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Asn-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2;RPGPPGLQGRLQRLLQANGNHAAGILTM-NH2.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Orexin B, rat, mouse Chemical Structure

Orexin B, rat, mouse Chemical Structure

CAS No. : 202801-92-1

Size Price Stock Quantity
500 μg USD 190 In-stock
Estimated Time of Arrival: December 31
1 mg USD 290 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1150 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Orexin B, rat, mouse is an endogenous agonist at Orexin receptor with Kis of 420 and 36 nM for OX1 and OX2, respectively. Sequence: Arg-Pro-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Asn-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2;RPGPPGLQGRLQRLLQANGNHAAGILTM-NH2.

IC50 & Target

Ki: 420 nM (OX1), 36 nM (OX2)[1]

In Vitro

Orexin B is derived by proteolytic amino acid precursor, prepro-orexin, which is encoded by a gene localized to chromosome 17q21 in humans. In radioligand binding studies, Orexin B has a higher affinity for the OX2 receptor[1]. Human Orexin B has two amino acid substitutions when compared with the rodent sequence. OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC50 of 36 nM in the binding assay and an EC50 of 60 nM in the [Ca2+]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC50 in the competitive binding assay and the EC50 in the [Ca2+]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively[2].

In Vivo

Human Orexin B significantly augments food intake; at the 2 hr time point, 5- and 12-fold stimulation of food consumption is observed by 3 nM and 30 nM orexin-B, respectively[2].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3406 mL 1.7028 mL 3.4055 mL
5 mM 0.0681 mL 0.3406 mL 0.6811 mL
10 mM 0.0341 mL 0.1703 mL 0.3406 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[2]

Rats[2]

3 nM and 30 nM of synthetic human Orexin B is administered in a 5 mL bolus through a catheter placed in the left lateral ventricle of Male Wistar rats (180–200 g) in early light phase. Cumulative food consumption is observed and plotted over the period of 4 hr after injection[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

2936.40

Formula

C₁₂₆H₂₁₅N₄₅O₃₄S

CAS No.

202801-92-1

Storage
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Orexin B, rat, mouse
Cat. No.:
HY-P1349
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Orexin B, rat, mouse

Cat. No.: HY-P1349