1. Apoptosis
  2. Apoptosis

Osajin (Synonyms: CID 95168; NSC 21565)

Cat. No.: HY-N3125
Handling Instructions

Osajin is the major bioactive isoflavone present in the fruit of Maclura pomifera with antitumor, antioxidant and anti-inflammatory activities.

For research use only. We do not sell to patients.

Osajin Chemical Structure

Osajin Chemical Structure

CAS No. : 482-53-1

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Osajin is the major bioactive isoflavone present in the fruit of Maclura pomifera with antitumor, antioxidant and anti-inflammatory activities.

IC50 & Target

Apoptosis[1]

In Vitro

Osajin significantly decreases the viability of human NPC cells (TW076, CG1 and TW04 cells) in a dose-dependent manner. Osajin induces apoptosis in human NPC cells through multiple apoptotic pathways, including the extrinsic death receptor pathway, and intrinsic pathways relying on mitochondria and endoplasmic reticulum stress[1]. Osajin exhibits growth inhibitory activity on six human cancer cell lines, including kidney, lung, prostate, breast, melanoma and colon cancer cells[2].

In Vivo

Osajin and pomiferin attenuates the myocardial dysfunction provoked by ischemia reperfusion. This is confirmed by an increase in both antioxidant enzyme values and total antioxidant activity. The cardioprotection provided by osajin and pomiferin treatment results from the suppression of oxidative stress and this correlates with improved ventricular function[3].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4724 mL 12.3622 mL 24.7243 mL
5 mM 0.4945 mL 2.4724 mL 4.9449 mL
10 mM 0.2472 mL 1.2362 mL 2.4724 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

NPC cells are seeded in 96-well plates (5000/well) and allowed to adhere for 24 h. The medium is then substituted by a fresh one containing different concentrations (0.01–10 µM) of osajin for another 24 h. For the time-course assay, the incubation times with 5 µM of osajin are 6, 12, 24, 36 and 48 h. After incubation, 10 µl of 5 mg/ml of MTT is added to each well and incubated for 4 h at 37°C. Cell viability is determined using the MTT-based assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Rats[1]

The Wistar rats are divided into four groups. The first treatment group receives osajin (5 mg/kg/day in 0.5% Avicel); the second treatment group receives pomiferin (5 mg/kg/day in 0.5% Avicel); the placebo group receives only 0.5 Avicel; the last is an untreated control group. Biochemical indicator of oxidative damage-lipid peroxidation product malondialdehyde, antioxidant enzymes-superoxide dismutase, glutathione peroxidase, total antioxidant activity in serum and myocardium are evaluated. The effect of osajin and pomiferin on cardiac function, left ventricular end-diastolic pressure, left ventricular pressure and peak positive +dP/dt ischemia and reperfusion, also is examined[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

404.46

Formula

C₂₅H₂₄O₅

CAS No.

482-53-1

SMILES

O=C1C2=C(O)C(C/C=C(C)\C)=C3C(C=CC(C)(C)O3)=C2OC=C1C4=CC=C(O)C=C4

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Osajin
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Osajin

Cat. No.: HY-N3125