1. Anti-infection
  2. Bacterial

PNU-176798 

Cat. No.: HY-100306
Handling Instructions

PNU-176798 is an antimicrobial agent, targeting protein synthesis in a wide spectrum of gram-positive and anaerobic bacteria.

For research use only. We do not sell to patients.

PNU-176798 Chemical Structure

PNU-176798 Chemical Structure

CAS No. : 428861-91-0

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1 mg USD 939 Get quote
5 mg USD 1502 Get quote
10 mg USD 2404 Get quote

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

PNU-176798 is an antimicrobial agent, targeting protein synthesis in a wide spectrum of gram-positive and anaerobic bacteria.

IC50 & Target

Bacterial[1]

In Vitro

PNU-176798 is an antimicrobial agent, with a minimum inhibitory concentration (MIC) of 1.4 μM for E. coli. PNU-176798 inhibits fMet-tRNA binding to the 70S ribosomes, with an IC50 of 32 μM. PNU-176798 also blocks translation, 70S initiation with IC50s of 0.53, and 32 μM, respectively. PNU-176798 inhibits peptidyl transferase (IC50, 40 μM), and the inhibition is more pronounced in the presence of elongation factor P (EF-P). PNU-176798 markedly inhibits the EF-G-mediated translocation of fMet-tRNA (IC50, 8 μM)[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7750 mL 13.8750 mL 27.7500 mL
5 mM 0.5550 mL 2.7750 mL 5.5500 mL
10 mM 0.2775 mL 1.3875 mL 2.7750 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

Binding of the f[35S]Met-tRNA to E. coli 70S ribosomes is conducted and the initiation reaction mixtures are prepared without initiation factors and contain 6 mM magnesium acetate [Mg(Ac)2], 0.08 μM AUG, 30 mM NH4Cl, 10 mM Tris (pH 7.4), and 20 pmol of 70S ribosomes in a final volume of 60 μL. The reaction mixtures are incubated for 15 min at 35°C, and the reactions are terminated by addition of cold buffer A, washed with buffer A, and counted by liquid scintillation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

360.36

Formula

C₁₆H₁₃FN₄O₃S

CAS No.

428861-91-0

SMILES

CC(NC[[email protected]]1CN(C2=CC=C(C3=NC=C(C#N)S3)C(F)=C2)C(O1)=O)=O

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
PNU-176798
Cat. No.:
HY-100306
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PNU-176798

Cat. No.: HY-100306