SB-399885 hydrochloride

Name: SB-399885 hydrochloride
Brand: MedChemExpress
SKU: HY-103099
Rating: 4.5 (1 reviews)

Cat. No.: HY-103099 Purity: 99.58%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SB-399885 hydrochloride is a 5-HT₆ receptor antagonist.

For research use only. We do not sell to patients.

SB-399885 hydrochloride Chemical Structure

CAS No. : 402713-81-9

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 119	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 119	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 108	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 408	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 732	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1320	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of SB-399885 hydrochloride:

SB399885 Get quote

1 Publications Citing Use of MCE SB-399885 hydrochloride

•Integr Zool. 2023 Jan 7. [Abstract]

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All 5-HT Receptor Isoform Specific Products:

View All Isoforms

5-HT₁ Receptor 5-HT₂ Receptor 5-HT₃ Receptor 5-HT₄ Receptor 5-HT₅ Receptor 5-HT₆ Receptor 5-HT₇ Receptor 5-HT Receptor

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

SB-399885 hydrochloride is a 5-HT₆ receptor antagonist.

IC₅₀ & Target^[1]

5-HT₆ Receptor

In Vivo

Compare with the control vehicle SB-399885 hydrochloride 10 mg/kg significantly increases wakefulness (W) (F_(3,15)=3.32, P<0.05) while slow wave sleep (SWS), rapid-eye-movement sleep (REMS) and the number of REM periods are reduced (F_(3,15)=4.0, P<0.01; F_(3,15)=3.14, P<0.05 and F_(3,15)=2.62, P<0.05, respectively). Analysis of sleep variables in 2-h blocks shows that SB-399885 hydrochloride 10 mg/kg increases W (F_(3,15)=5.48, P<0.01) and reduces SWS (F_(3,15)=5.42, P<0.01) and REMS (F_(3,15)= 4.05, P<0.01) during the first 2-h period. SB-399885 hydrochloride 5 and 10 mg/kg augment light sleep over the first (F_(3,15)=3.46,P<0.01 and F_(3,15)= 3.65, P<0.01, respectively) and the second (F_(3,15)=3.23, P<0.05 and F_(3,15)=3.08, P<0.05, respectively) 2-h recording periods. SB-399885 hydrochloride 10 mg/kg significantly increases REMS latency (F_(3,15)=3.60, P<0.01) and reduces the number of REM periods during the first 2-h of recording (F_(3,15)=3.88, P<0.01)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

482.81

Formula

C₁₈H₂₂Cl₃N₃O₄S

CAS No.

402713-81-9

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(OC)C(N2CCNCC2)=C1)(NC3=CC(Cl)=CC(Cl)=C3OC)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (207.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 6.67 mg/mL (13.81 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0712 mL	10.3560 mL	20.7121 mL
5 mM	0.4142 mL	2.0712 mL	4.1424 mL
10 mM	0.2071 mL	1.0356 mL	2.0712 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.18 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.18 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (251 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Monti JM, et al. Effects of the 5-HT6 receptor antagonists SB-399885 and RO-4368554 and of the 5-HT(2A) receptor antagonist EMD 281014 on sleep and wakefulness in the rat during both phases of the light-dark cycle. Behav Brain Res. 2011 Jan 1;216(1):381-8 [Content Brief]

Animal Administration
^[1]

Twelve male Wistar rats weighing 350 to 400 g at the time of surgery are used. SB-399885 hydrochloride 2.5, 5 and 10 mg/kg or vehicle (1% aqueous solution of Tween 80) (n=6) are administered intraperitoneally in animals adapted to a 12 h dark/12 h light cycle for 4 weeks, starting 2 h after the beginning of the dark period. Each animal receives all 12 treatments. Recordings are begun 15 min later and continued for 6 h. The control solution and SB-399885 hydrochloride are given at least three days apart^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Monti JM, et al. Effects of the 5-HT6 receptor antagonists SB-399885 and RO-4368554 and of the 5-HT(2A) receptor antagonist EMD 281014 on sleep and wakefulness in the rat during both phases of the light-dark cycle. Behav Brain Res. 2011 Jan 1;216(1):381-8 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0712 mL	10.3560 mL	20.7121 mL	51.7802 mL
	5 mM	0.4142 mL	2.0712 mL	4.1424 mL	10.3560 mL
	10 mM	0.2071 mL	1.0356 mL	2.0712 mL	5.1780 mL
DMSO	15 mM	0.1381 mL	0.6904 mL	1.3808 mL	3.4520 mL
	20 mM	0.1036 mL	0.5178 mL	1.0356 mL	2.5890 mL
	25 mM	0.0828 mL	0.4142 mL	0.8285 mL	2.0712 mL
	30 mM	0.0690 mL	0.3452 mL	0.6904 mL	1.7260 mL
	40 mM	0.0518 mL	0.2589 mL	0.5178 mL	1.2945 mL
	50 mM	0.0414 mL	0.2071 mL	0.4142 mL	1.0356 mL
	60 mM	0.0345 mL	0.1726 mL	0.3452 mL	0.8630 mL
	80 mM	0.0259 mL	0.1295 mL	0.2589 mL	0.6473 mL
	100 mM	0.0207 mL	0.1036 mL	0.2071 mL	0.5178 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.