1. GPCR/G Protein Neuronal Signaling
  2. Dopamine Receptor
  3. SKF 38393 hydrochloride

SKF 38393 hydrochloride  (Synonyms: (±)-SKF-38393 hydrochloride; SKF-38393A)

Cat. No.: HY-12520A Purity: 99.44%
COA Handling Instructions

SKF 38393 hydrochloride is a selective agonist of the dopamine D1 receptor (D1DR) with an IC50 of 110 nM.

For research use only. We do not sell to patients.

SKF 38393 hydrochloride Chemical Structure

SKF 38393 hydrochloride Chemical Structure

CAS No. : 62717-42-4

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Solid + Solvent
10 mM * 1 mL in DMSO
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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of SKF 38393 hydrochloride:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SKF 38393 hydrochloride is a selective agonist of the dopamine D1 receptor (D1DR) with an IC50 of 110 nM[1].

IC50 & Target

D1 Receptor

 

In Vitro

SKF-38393 hydrochloride induces a similar change in cytomorphology and increases the levels of media cAMP[2].
? SKF-38393 hydrochloride (10 μmol/L; 1 hour) induces increased threonine-phosphorylation of DA- and cAMP-regulated phosphoprotein of Mr 32 kD (DARPP-32) in cultured GC cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2].

Cell Line: GC cells
Concentration: 10 μmol/L
Incubation Time: 1 hour
Result: Induced increased threonine-phosphorylation of DA- and cAMP-regulated phosphoprotein of Mr 32 kD (DARPP-32) in cultured GC cells.
In Vivo

SKF-38393 hydrochloride (10 mg/kg; i.p.) blocks the 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) -induced depletion of glutathione[3].
? SKF-38393 hydrochloride attenuates MPTP-induced depletion of dopamine[3].
? SKF-38393 hydrochloride enhances the activity of superoxide dismutase and hence mimics the action of Selegiline[3].
? SKF-38393 hydrochloride enhances the frequency but not the amplitude of tetrodotoxin-resistant excitatory postsynaptic currents which argues for a presynaptic locus of D1 action[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c mice ( 20-25 g)[3].
Dosage: 5 mg/kg, 10 mg/kg
Administration: Intraperitoneal injection
Result: Blocked the MPTP-induced depletion of glutathione and attenuated MPTP-induced depletion of dopamine.
Molecular Weight

291.77

Formula

C16H18ClNO2

CAS No.
Appearance

Solid

Color

Light yellow to khaki

SMILES

OC1=C(O)C=C2C(C3=CC=CC=C3)CNCCC2=C1.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 34 mg/mL (116.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 10 mg/mL (34.27 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4274 mL 17.1368 mL 34.2736 mL
5 mM 0.6855 mL 3.4274 mL 6.8547 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.13 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.44%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.4274 mL 17.1368 mL 34.2736 mL 85.6839 mL
5 mM 0.6855 mL 3.4274 mL 6.8547 mL 17.1368 mL
10 mM 0.3427 mL 1.7137 mL 3.4274 mL 8.5684 mL
15 mM 0.2285 mL 1.1425 mL 2.2849 mL 5.7123 mL
20 mM 0.1714 mL 0.8568 mL 1.7137 mL 4.2842 mL
25 mM 0.1371 mL 0.6855 mL 1.3709 mL 3.4274 mL
30 mM 0.1142 mL 0.5712 mL 1.1425 mL 2.8561 mL
DMSO 40 mM 0.0857 mL 0.4284 mL 0.8568 mL 2.1421 mL
50 mM 0.0685 mL 0.3427 mL 0.6855 mL 1.7137 mL
60 mM 0.0571 mL 0.2856 mL 0.5712 mL 1.4281 mL
80 mM 0.0428 mL 0.2142 mL 0.4284 mL 1.0710 mL
100 mM 0.0343 mL 0.1714 mL 0.3427 mL 0.8568 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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SKF 38393 hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SKF 38393 hydrochloride
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