Zearalenone (Synonyms: Mycotoxin F2; Toxin F2)

Name: Zearalenone
Brand: MedChemExpress
SKU: HY-103447
Rating: 5.0 (10 reviews)

Cat. No.: HY-103447 Purity: 98.95%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Zearalenone is a mycotoxin produced mainly by fungi belonging to the genus Fusarium in foods and feeds. Possess oestrogenic activity in pigs, cattle and sheep, with low acute toxicity. Causes precocious development of mammae and other estrogenic effects in young gilts.

For research use only. We do not sell to patients.

CAS No. : 17924-92-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Zearalenone:

10 Publications Citing Use of MCE Zearalenone

In Vivo Efficacy Study

Cell Imaging/Staining

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

: Zearalenone purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Sep 15:303:119045. [Abstract]; Cells were pretreated with the indicated concentrations of LYC and then stimulated with 30 μM Zearalenone for 24 h. The cell viability was detected.

: Zearalenone purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Sep 15:303:119045. [Abstract]; AML-12 cells were pretreated with 20 μM LYC or/and 5 μM ML385 for 2 h, followed by incubation with 30 μM Zearalenone for 24 h. Nuclear proteins were isolated, and Nrf2 activity was detected.

: Zearalenone purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Sep 15:303:119045. [Abstract]; AML-12 cells were pretreated with 20 μM LYC or/and 10 μM Compound C for 2 h, followed by incubation with 30 μM Zearalenone for 24 h. p-Nrf2 protein expression.

: Zearalenone purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Aug 24:284:116914. [Abstract]; The effect of different concentrations of Zearalenone (0-100 μM) on the viability of IPEC-J2 cells was detected by CCK-8 assay.

: Zearalenone purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Aug 24:284:116914. [Abstract]; Protective effects of different concentrations of SeNPs against Zearalenone (0-50 μM)-induced cytotoxicity.

: Zearalenone purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Aug 24:284:116914. [Abstract]; Cellular morphology and apoptosis of IPEC-J2 cells exposed to Zearalenone and (or) SeNPs. Apoptotic cells have bright blue fluorescence (red arrows).

: Zearalenone purchased from MedChemExpress. Usage Cited in: Biomedicines. 2024 Apr 24;12(5):943.; Mean (±SE) expression level of Cyp19α1 in rats exposed to zearalenone 0.1 mg/kg b.w. (Zearalenone), hispidin (His), and/or magnesium oxide nanoparticles (MgONPs) and Zearalenone treated with hispidin (Zearalenone + His) or combined hispidin and magnesium oxide nanoparticles (Zearalenone + His + MgONPs). Different letters indicate significant differences between groups (p ≤ 0.05).

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Estrogen Receptor/ERR Isoform Specific Products:

View All Isoforms

Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	138.21 μM Compound: 1	Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27311894]
KB	IC₅₀	46.3 μM Compound: 1	Cytotoxicity against human KB cells assessed as reduction in cell viability by resazurin microplate assay Cytotoxicity against human KB cells assessed as reduction in cell viability by resazurin microplate assay	[PMID: 27311894]
MCF7	IC₅₀	104.72 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability by resazurin microplate assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability by resazurin microplate assay	[PMID: 27311894]
MCF7	IC₅₀	> 25 μM Compound: 8	Cytotoxicity against human MCF7 cells after 72 hrs by MTS assay Cytotoxicity against human MCF7 cells after 72 hrs by MTS assay	[PMID: 21513293]
NCI-H460	IC₅₀	> 25 μM Compound: 8	Cytotoxicity against human H460 cells after 72 hrs by MTS assay Cytotoxicity against human H460 cells after 72 hrs by MTS assay	[PMID: 21513293]
SF-268	IC₅₀	> 50 μM Compound: 8	Cytotoxicity against human SF268 cells after 72 hrs by MTS assay Cytotoxicity against human SF268 cells after 72 hrs by MTS assay	[PMID: 21513293]
Vero	IC₅₀	40.58 μM Compound: 1	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability by green fluorescent protein-based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability by green fluorescent protein-based assay	[PMID: 27311894]

In Vivo

Zearalenone is frequently implicated in reproductive disorders of farm animals and occasionally in hyperoestrogenic syndromes in humans. There is evidence that ZEA and its metabolites possess oestrogenic activity in pigs, cattle and sheep. However, ZEA is of a relatively low acute toxicity after oral or interperitoneal administration in mice, rat and pig^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

318.36

Formula

C₁₈H₂₂O₅

CAS No.

17924-92-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@@H](C)CCC1)C2=C(O)C=C(O)C=C2/C=C/CCCC1=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Fusarium

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (314.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1411 mL	15.7055 mL	31.4110 mL
5 mM	0.6282 mL	3.1411 mL	6.2822 mL
10 mM	0.3141 mL	1.5705 mL	3.1411 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.95%

Select Batch:

Data Sheet (274 KB) SDS (641 KB)

COA (249 KB) RP-HPLC (213 KB)

Handling Instructions (2659 KB)

References

[1]. Zinedine A, et al. Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. Food Chem Toxicol. 2007 Jan;45(1):1-18. [Content Brief]

[2]. Richard JL, et al. Some major mycotoxins and their mycotoxicoses--an overview. Int J Food Microbiol. 2007 Oct 20;119(1-2):3-10. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1411 mL	15.7055 mL	31.4110 mL	78.5275 mL
	5 mM	0.6282 mL	3.1411 mL	6.2822 mL	15.7055 mL
	10 mM	0.3141 mL	1.5705 mL	3.1411 mL	7.8527 mL
	15 mM	0.2094 mL	1.0470 mL	2.0941 mL	5.2352 mL
	20 mM	0.1571 mL	0.7853 mL	1.5705 mL	3.9264 mL
	25 mM	0.1256 mL	0.6282 mL	1.2564 mL	3.1411 mL
	30 mM	0.1047 mL	0.5235 mL	1.0470 mL	2.6176 mL
	40 mM	0.0785 mL	0.3926 mL	0.7853 mL	1.9632 mL
	50 mM	0.0628 mL	0.3141 mL	0.6282 mL	1.5705 mL
	60 mM	0.0524 mL	0.2618 mL	0.5235 mL	1.3088 mL
	80 mM	0.0393 mL	0.1963 mL	0.3926 mL	0.9816 mL
	100 mM	0.0314 mL	0.1571 mL	0.3141 mL	0.7853 mL

Zearalenone Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Zearalenone17924-92-4Mycotoxin F2 Toxin F2Mycotoxin F 2Mycotoxin F-2Toxin F2Toxin F 2Toxin F-2Estrogen Receptor/ERRInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Zearalenone (Synonyms: Mycotoxin F2; Toxin F2)

Customer Review

Other Forms of Zearalenone:

Zearalenone Related Antibodies

10 Publications Citing Use of MCE Zearalenone

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Estrogen Receptor/ERR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Zearalenone Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report