Ac-QPKK(Ac)-AMC acetate 

Ac-QPKK(Ac)-AMC acetate is a p53-derived peptide conjugated with a fluorophore, which serves as a fluorescent peptide substrate for detecting the deacylase activity of zinc-dependent HDACs and sirtuins (Ex = 360 nm; Em = 460 nm)^[1].

Ac-QPKK(Ac)-AMC (4a) (50 μM; 60 min) acetate is acted upon by recombinant human HDAC1, HDAC2, and HDAC3/NCoR2 which exhibit potent deacetylase activity toward the peptide substrate in vitro, with HDAC3/NCoR2 being the most potent^[1].
Ac-QPKK(Ac)-AMC (2a) acetate is deacetylated by recombinant SIRT1, SIRT2 and SIRT3 in vitro with distinct catalytic efficiencies, whereas SIRT6 shows negligible deacetylase activity toward this substrate^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

740.85 (free base)

C₃₆H₅₂N₈O₉·xC₂H₄O₂

Solid

White to off-white

Ac-Gln-Pro-Lys-{Lys(Ac)}-AMC

Ac-QPK-{Lys(Ac)}-AMC

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Moreno-Yruela C, et al. Class I histone deacetylases (HDAC1–3) are histone lysine delactylases. Sci Adv. 2022 Jan 19;8(3):eabi6696.

  [2]. Galleano I, et al. A Continuous, Fluorogenic Sirtuin 2 Deacylase Assay: Substrate Screening and Inhibitor Evaluation. J Med Chem. 2016;59(3):1021-1031.  [Content Brief]