ALDH3A1-IN-4
Based on 1 Customer Validation
ALDH3A1-IN-4 is a selective ALDH3A1 inhibitor with an IC50 of 0.2 μM. ALDH3A1-IN-4 binds to the aldehyde-binding pocket of ALDH3A1 via hydrophobic interactions and van der Waals forces. ALDH3A1-IN-4 acts as a chemosensitizer that sensitizes ALDH3A1-expressing cancer cells to the antiproliferative effect of mafosfamide, but does not produce a sensitizing effect on non-cancer cells that do not express ALDH3A1. ALDH3A1-IN-4 is applicable to research related to lung adenocarcinoma and glioblastoma.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.91%
- CAS. Nr.: 324777-01-7
- Formel: C14H11FN2O2S
- Molecular Weight:290.31
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
ALDH3A1 0.2 μM (IC50) |
In Vitro
ALDH3A1-IN-4 (compound CB7) (10 μM; 1 min) potently inhibits ALDH3A1 activity in ALDH3A1-expressing cancer cell lysates[1].
ALDH3A1-IN-4 (10 μM; 19 h) selectively sensitizes ALDH3A1-expressing A549 and SF767 cancer cells to mafosfamide[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 lung adenocarcinoma, SF767 glioblastoma, CCD-13Lu primary lung fibroblast cell lines
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Concentration:10 μM (in combination with mafosfamide)
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Incubation Time:19 h
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Result:Decreased cell proliferation in A549/SF767 cells compared to mafosfamide alone, and no increased chemosensitization was observed in CCD-13Lu cells treated with ALDH3A1-IN-4 and mafosfamide.
Chemical Information
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CAS. Nr. 324777-01-7
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Appearance Solid
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Molecular Weight 290.31
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Formel C14H11FN2O2S
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Color White to off-white
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SMILES
O=S(=O)(C1=CC=C(F)C=C1)N2C=3C=CC=CC3N=C2C
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (344.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.4446 mL | 17.2230 mL | 34.4459 mL | 86.1148 mL |
| 5 mM | 0.6889 mL | 3.4446 mL | 6.8892 mL | 17.2230 mL | |
| 10 mM | 0.3445 mL | 1.7223 mL | 3.4446 mL | 8.6115 mL | |
| 15 mM | 0.2296 mL | 1.1482 mL | 2.2964 mL | 5.7410 mL | |
| 20 mM | 0.1722 mL | 0.8611 mL | 1.7223 mL | 4.3057 mL | |
| 25 mM | 0.1378 mL | 0.6889 mL | 1.3778 mL | 3.4446 mL | |
| 30 mM | 0.1148 mL | 0.5741 mL | 1.1482 mL | 2.8705 mL | |
| 40 mM | 0.0861 mL | 0.4306 mL | 0.8611 mL | 2.1529 mL | |
| 50 mM | 0.0689 mL | 0.3445 mL | 0.6889 mL | 1.7223 mL | |
| 60 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4352 mL | |
| 80 mM | 0.0431 mL | 0.2153 mL | 0.4306 mL | 1.0764 mL | |
| 100 mM | 0.0344 mL | 0.1722 mL | 0.3445 mL | 0.8611 mL |
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