AM73
AM73 is a PGC-1α1 stabilizer. AM73 increases the expression levels of Ucp1 and Cidea in brown adipocytes, and enhances basal mitochondrial respiration and uncoupled respiration. AM73 regulates hypoxia, brown adipocyte differentiation, carbohydrate metabolism and insulin signaling pathways in brown adipocytes. AM73 alters the thermogenic process of white adipocytes. AM73 is applicable to the research of obesity and related metabolic disorders.
For research use only. We do not sell to patients.
- CAS No.: 931619-99-7
- Formula: C18H13ClN2O5
- Molecular Weight:372.76
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
AM73 (10 μM; 8 h) increases the level of EGFP-PGC-1α1 fusion protein in HEK 293-EGFPmPGC-1α1 cells[1].
AM73 (10 μM; 8 h) induces significant expression of thermogenic and mitochondrial PGC-1α target genes in fully differentiated immortalized mouse brown adipocytes[1].
AM73 (10 μM; 8 h) stabilizes endogenous PGC-1α protein in fully differentiated immortalized mouse brown adipocytes[1].
AM73 (10 μM; 8 h) increases basal mitochondrial respiration and uncoupled mitochondrial respiration in fully differentiated immortalized mouse brown adipocytes by approximately 13%[1].
AM73 (10 μM; 8 h) regulates numerous PGC-1α-associated gene pathways related to thermogenesis, metabolism and differentiation in fully differentiated immortalized mouse brown adipocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human embryonic kidney (HEK) 293-EGFPmPGC-1α1 stable cells (expressing EGFP-PGC-1α1 fusion protein)
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Concentration:10 μM
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Incubation Time:8 h
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Result:Induced a 206.1% EGFP intensity increase relative to DMSO control, as measured by whole cell integrated fluorescence intensity median per well.
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Cell Line:fully differentiated mouse immortalized brown adipocytes
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Concentration:10 μM
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Incubation Time:8 h
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Result:Induced a 12-fold increase in Ucp1 gene expression, a ~4-fold increase in Cidea gene expression, and modest (≤2-fold) increases in the expression of other PGC-1α target genes including Tfam, Esrra, Prdm16, and Sod1, relative to DMSO control.
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Cell Line:fully differentiated mouse immortalized brown adipocytes
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Concentration:10 μM
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Incubation Time:8 h
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Result:Increased endogenous PGC-1α protein accumulation to levels comparable to cold-induced brown adipose tissue, as detected by immunoblotting.
Chemical Information
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CAS No. 931619-99-7
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Molecular Weight 372.76
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Formula C18H13ClN2O5
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SMILES
OC1=C(C(C2=CN(C3=NOC(C)=C3)C(C(C(C)=O)=C2)=O)=O)C=C(Cl)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AM73 Related Classifications
HY-183094 Related Classifications
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