2. Metabolic Enzyme/Protease
  3. PGC-1α
  4. AM73

AM73 is a PGC-1α1 stabilizer. AM73 increases the expression levels of Ucp1 and Cidea in brown adipocytes, and enhances basal mitochondrial respiration and uncoupled respiration. AM73 regulates hypoxia, brown adipocyte differentiation, carbohydrate metabolism and insulin signaling pathways in brown adipocytes. AM73 alters the thermogenic process of white adipocytes. AM73 is applicable to the research of obesity and related metabolic disorders.

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AM73

AM73 Chemical Structure

CAS No. : 931619-99-7

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AM73 Related Antibodies

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Description

AM73 is a PGC-1α1 stabilizer. AM73 increases the expression levels of Ucp1 and Cidea in brown adipocytes, and enhances basal mitochondrial respiration and uncoupled respiration. AM73 regulates hypoxia, brown adipocyte differentiation, carbohydrate metabolism and insulin signaling pathways in brown adipocytes. AM73 alters the thermogenic process of white adipocytes. AM73 is applicable to the research of obesity and related metabolic disorders[1].

In Vitro

AM73 (10 μM; 8 h) increases the level of EGFP-PGC-1α1 fusion protein in HEK 293-EGFPmPGC-1α1 cells[1].
AM73 (10 μM; 8 h) induces significant expression of thermogenic and mitochondrial PGC-1α target genes in fully differentiated immortalized mouse brown adipocytes[1].
AM73 (10 μM; 8 h) stabilizes endogenous PGC-1α protein in fully differentiated immortalized mouse brown adipocytes[1].
AM73 (10 μM; 8 h) increases basal mitochondrial respiration and uncoupled mitochondrial respiration in fully differentiated immortalized mouse brown adipocytes by approximately 13%[1].
AM73 (10 μM; 8 h) regulates numerous PGC-1α-associated gene pathways related to thermogenesis, metabolism and differentiation in fully differentiated immortalized mouse brown adipocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AM73 Related Antibodies

Immunofluorescence[1]

Cell Line: human embryonic kidney (HEK) 293-EGFPmPGC-1α1 stable cells (expressing EGFP-PGC-1α1 fusion protein)
Concentration: 10 μM
Incubation Time: 8 h
Result: Induced a 206.1% EGFP intensity increase relative to DMSO control, as measured by whole cell integrated fluorescence intensity median per well.

Real Time qPCR[1]

Cell Line: fully differentiated mouse immortalized brown adipocytes
Concentration: 10 μM
Incubation Time: 8 h
Result: Induced a 12-fold increase in Ucp1 gene expression, a ~4-fold increase in Cidea gene expression, and modest (≤2-fold) increases in the expression of other PGC-1α target genes including Tfam, Esrra, Prdm16, and Sod1, relative to DMSO control.

Western Blot Analysis[1]

Cell Line: fully differentiated mouse immortalized brown adipocytes
Concentration: 10 μM
Incubation Time: 8 h
Result: Increased endogenous PGC-1α protein accumulation to levels comparable to cold-induced brown adipose tissue, as detected by immunoblotting.
Molecular Weight

372.76

Formula

C18H13ClN2O5
CAS No.
SMILES

OC1=C(C(C2=CN(C3=NOC(C)=C3)C(C(C(C)=O)=C2)=O)=O)C=C(Cl)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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AM73 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AM73931619-99-7AM 73AM-73PGC-1αPPARγ coactivator-1αPGC-1α1 stabilizerHEK 293-EGFPmPGC-1α1 cellsmouse brown adipocytesobesityInhibitorinhibitorinhibit

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