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Cat. No.: HY-183097

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AM89

AM89 is a PGC-1α1 stabilizer. AM89 activates the expression of PGC-1α1 target genes and induces the upregulation of Ucp1 gene expression. AM89 is applicable for the research of obesity and related metabolic disorders.

For research use only. We do not sell to patients.

Formula: C₂₂H₁₉NO₄S
Molecular Weight:393.46

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

AM89 Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

AM89 (10 μM; 8 h) increases EGFP-PGC-1α1 fusion protein levels in stable HEK 293-EGFPmPGC-1α1 cells, with an EGFP intensity of 143.3%^[1].
AM89 (10 μM; 8 h) induces Ucp1 gene expression 5-fold and modestly increases other PGC-1α1 target gene expression in fully differentiated mouse brown adipocytes^[1].
AM89 (10 μM; 8 h) stabilizes endogenous PGC-1α1 protein in fully differentiated mouse brown adipocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

fully differentiated immortalized mouse brown adipocytes
Concentration:

10 μM
Incubation Time:

8 h
Result:

Induced a 5-fold increase in Ucp1 gene expression relative to DMSO-treated controls.
Induced small increases in the expression of other PGC-1α1 target genes including Tra2, Fsp27, Cytc, Tfam, Cpt1b, Esrra, Glut4, Prdm16, Pparg, and Sod1, though these increases were less pronounced than the Ucp1 induction.

Western Blot Analysis^[1]

Cell Line:

fully differentiated immortalized mouse brown adipocytes
Concentration:

10 μM
Incubation Time:

8 h
Result:

Stabilized endogenous PGC-1α1 protein in brown adipocytes, with protein accumulation levels comparable to other validated PGC-1α1 stabilizers.

Chemical Information

Molecular Weight 393.46
Formula C₂₂H₁₉NO₄S
SMILES

CC1C(/C=C2C(N(CC3=CC=C(OC)C=C3)C(S/2)=O)=O)=CC(C=CC=C4)=C4O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Pettersson-Klein AT, et al. Small molecule PGC-1α1 protein stabilizers induce adipocyte Ucp1 expression and uncoupled mitochondrial respiration. Mol Metab. 2018;9:28-42. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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