Anti-IL-4 Antibody (MP4-25D2)
Based on 1 Customer Validation
Anti-IL-4 Antibody (MP4-25D2) is a kind of rat IgG1 κ chimeric antibody inhibitor, targeting to human IL-4. Anti-IL-4 Antibody (MP4-25D2) can neutralize human IL-4 (interleukin-4). Anti-IL-4 Antibody (MP4-25D2) can be used for the inflammation and immunology.
For research use only. We do not sell to patients.
- Purity: ≥95.0%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Rat IgG1 kappa
Human
IL-4
Anti-IL-4 Antibody (MP4-25D2) (10 μg/mL) induces human CD4+ T cells to differentiate into Th1 cells[1].
Anti-IL-4 Antibody (MP4-25D2) (10 μg/mL, 48 h) reduces IL-9 levels in human CD4+ T cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
in vitro IL-4 neutralization; Flow cytometry
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Immobilized IL-4 Protein, Human, HY-P70445 can bind Anti-IL-4 Antibody (MP4-25D2). The EC50 for this effect is 24.47 ng/mL. -
Flow Cytometry analysis of Raji cells labelling IL-4(red) with Anti-IL-4 Antibody (MP4-25D2) (anti-IL4) (HY-P990837). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rat IgG H&L (AF488) (HY-P89199) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG1 kappa (HY-P99979, blue) was used as the isotype control.
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-IL-4 Antibody (MP4-25D2)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (257 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Li L, Liu X, et al. TLR8-Mediated Metabolic Control of Human Treg Function: A Mechanistic Target for Cancer Immunotherapy. Cell Metab. 2019 Jan 8;29(1):103-123.e5. [Content Brief]
[2]. Nakatsukasa H, et al. The DNA-binding inhibitor Id3 regulates IL-9 production in CD4(+) T cells. Nat Immunol. 2015 Oct;16(10):1077-84. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)