Anti-Mouse Galectin-9 Antibody (RG9-1)
Based on 1 Customer Validation
Anti-Mouse Galectin-9 Antibody (RG9-1) is an anti-mouse Galectin-9 IgG2b monoclonal antibody. Anti-Mouse Galectin-9 Antibody (RG9-1) promotes inflammatory response by enhancing the secretion of IFN-γ, TNF-α, and IL-6. Anti-Mouse Galectin-9 Antibody (RG9-1) can increase CD8 T cell and Treg frequency. Anti-Mouse Galectin-9 Antibody (RG9-1) reduces graft survival time by increasing CD4+ and CD8+ T cell infiltration. Anti-Mouse Galectin-9 Antibody (RG9-1) can be used for researches on inflammation conditions, cancer and xenotransplantation such as ischemia-reperfusion, colon cancer, breast cancer and leukemia.
For research use only. We do not sell to patients.
- Purity : 95.64%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Isotype
Rat IgG2b kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
[2]|
IL-6 |
In Vitro
Anti-Mouse Galectin-9 Antibody (RG9-1) (10 μg, 2 h) significantly increases the killing effect of allogeneic reactive T cells on corneal endothelial cells (CECs) and inhibits the apoptosis of allogeneic reactive CD4+ T cells[1].
Anti-Mouse Galectin-9 Antibody (RG9-1) (20 μg/mL) significantly increases the production of IFN-γ in splenic T cells stimulated by Concanavalin A (HY-P2149)[2].
Anti-Mouse Galectin-9 Antibody (RG9-1) (20 μg/mL) significantly increases the secretion of TNF-α and IL-6 in the co-culture system of macrophages and T cells[2].
Anti-Mouse Galectin-9 Antibody (RG9-1) can block Gal-9/TIM-3 interaction and reduce Jurkat T cell apoptosis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse Galectin-9 Antibody (RG9-1) (100 μg, i.p., once every 3 days, for 3 times) significantly enhances anti-tumor efficacy and prolongs survival combined with GITR mAb (HY-P990124) in female C57BL/6J mice bearing MC-38 tumors and BALB/c mice bearing EMT-6 tumors[3].
Anti-Mouse Galectin-9 Antibody (RG9-1) (6 mg/kg, i.p., 3 times every weeks, for 7 weeks) significantly delays the progression of leukemia in female C57BL/6 mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male BALB/c mice (8-10 weeks)[1]
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Dosage:0.2 mg
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Administration:Intraperitoneal injection (i.p.), 3 times a week, for 8 weeks
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Result:Significantly shortened the survival time of corneal grafts.
Significantly increased CD4+ and CD8+ T cell infiltration.
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Animal Model:3×105 MC38 cells injected female C57BL/6J mice and 1×105 EMT-6 cells injected BALB/c mice (4 weeks)[3]
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Dosage:100 μg, combined with GITR mAb (100 μg)
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Administration:Intraperitoneal injection (i.p.), once every 3 days, for 3 times
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Result:Significantly inhibited tumor volume.
Significantly prolonged the survival period of mice.
Increased the frequency of TIM-3+ CD8 T cells and Treg when used alone with RG9-1.
Cleared Treg and significantly increased CD8/Treg ratio combined with GITR mAb.
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Animal Model:1×107 TCL1 cells injected female C57BL/6 mice (8-12 weeks)[4]
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Dosage:6 mg/kg
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Administration:Intraperitoneal injection (i.p.), 3 times every weeks, for 7 weeks
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Result:Reduced the number of chronic lymphocytic leukemia (CLL) cells in peripheral blood.
Significantly reduced spleen weight.
Reduced the number of TIM3+ T cells and enhanced the function of CD8+ T cells.
Gene ID
Accession
O08573-2
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo Galectin-9 blockade; in vitro Galectin-9 blockade
Verified Bioactivity
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"Flow cytometric analysis of 1X106 NIH3T3 cells labeling Galectin-9 with Anti-Mouse Galectin-9 Antibody (HY-P990242, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 for an hour at 4℃. AF488-conjugated Goat Anti-Rat IgG H&L (AF488) (HY-P89199) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2b kappa Isotype Control (HY-P990682, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black)."
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse Galectin-9 Antibody (RG9-1)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Shimmura-Tomita M, et al. Galectin-9-mediated protection from allo-specific T cells as a mechanism of immune privilege of corneal allografts. PLoS One. 2013 May 7;8(5):e63620. [Content Brief]
[2]. Uchida Y, et al. T-cell immunoglobulin mucin-3 determines severity of liver ischemia/reperfusion injury in mice in a TLR4-dependent manner. Gastroenterology. 2010 Dec;139(6):2195-206. [Content Brief]
[3]. Yang R, et al. Galectin-9 interacts with PD-1 and TIM-3 to regulate T cell death and is a target for cancer immunotherapy. Nat Commun. 2021 Feb 5;12(1):832. [Content Brief]
[4]. Llaó-Cid L, et al. Integrative multi-omics reveals a regulatory and exhausted T-cell landscape in CLL and identifies galectin-9 as an immunotherapy target. Nat Commun. 2025 Aug 7;16(1):7271. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse Galectin-9 Antibody (RG9-1) Related Classifications
HY-P990242 Related Classifications
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