Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S)
Based on 1 Customer Validation
Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S) is an anti-mouse MHC Class I (H-2Kd, H-2Dd) IgG2a monoclonal antibody. Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S) can block the interaction between Ly49 and MHC-I. Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S) can be used for the construction of acute lung injury models.
For research use only. We do not sell to patients.
- Purity : 95.00%
- Molecular Weight:150 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Isotype
Mouse IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
|
MHC I |
In Vitro
Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S) can block the binding of Ly49 to MHC-I in splenocytes of mice[2].
Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S) (50 μg/well, 1 h) can inhibit TCR mediated T cell proliferation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Splenocytes of OT-I mice
-
Concentration:50 μg/well
-
Incubation Time:1 h
-
Result:Inhibited TCR mediated the cell proliferation.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Wild-type male C57BL/6 mice (8-10 weeks)[1]
-
Dosage:45 mg/kg, combined with MHC class I H-2Kb mAb (AF6-88.5.5.3) (4.5 mg/kg)
-
Administration:Intravenous injection (i.v.), single dose, 18 h after pre stimulation
-
Result:Caused pulmonary edema in the lungs of mice.
Led to the aggregation of polymorphonuclear neutrophils (PMNs) in the lungs.
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
-
Product Image
Application
in vivo activation of APCs
Chemical Information
-
Appearance Liquid
-
Molecular Weight 150 kDa
-
Color Colorless to light yellow
-
SMILES
[Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S)]
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (261 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Inhibitory Antibodies User Guide (603 KB)
References
[1]. Kapur R, et al. Osteopontin mediates murine transfusion-related acute lung injury via stimulation of pulmonary neutrophil accumulation. Blood. 2019 Jul 4;134(1):74-84. [Content Brief]
[2]. Panda AK, et al. Cutting Edge: Inhibition of the Interaction of NK Inhibitory Receptors with MHC Class I Augments Antiviral and Antitumor Immunity. J Immunol. 2020 Aug 1;205(3):567-572. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse MHC Class I (H-2Kd, H-2Dd) Antibody (34-1-2S) Related Classifications
HY-P990193 Related Classifications
Powered by Bioz