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CD47 Antibody (YA531)

(Synonyms: CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP)
Customer Review

Based on 1 Customer Validation

CD47 Antibody (YA531) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD47.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • CD47 Antibody (YA531) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000

Product Details

Description

CD47 Antibody (YA531) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD47.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database

Entrez Gene: 961 Human

SwissProt: Q08722 Human

Immunogen

Synthetic peptide corresponding to Human CD47.AA range:50-150.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102814

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD47 Antibody (YA531)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CD47 is a glycoprotein that was first identified in 1990 as a protein that associates with integrins, hence its original name integrin-associated protein. CD47 interacts with SIRPα (CD172a or SHPS-1) and its family member SIRPγ, CD47 also interacts with integrins and secreted glycoprotein extracellular matrix protein thrombospondin-1 (TSP-1) . Signaling through the CD47/SIRPα axis plays a role in various homeostatic processes, such as maintenance of erythrocytes, innate immune cells and T cells. Moreover, cancer cells and virus-infected and bacteria-infected cells can upregulate CD47 protein expression to prevent immune-mediated elimination.

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:They are widely distributed in normal adult tissues and ovarian tumors, especially abundant in certain epithelial tissues and brain tissues. Macrophages (PubMed: 39121194)

    Induction:Induced in pulmonary artery and lung parenchyma following injury or stress (PubMed:27742621) . Expression in arteries increases in normal aging (PubMed:32679764)

  • Isoforms & Post-Translational Modification

    Q08722 has 4 isomers: Q08722-1: 35214 Da (predicted); Q08722-2: 31743 Da (predicted); Q08722-3: 33192 Da (predicted); Q08722-4: 33845 Da (predicted).

  • Subunit

    Monomer (PubMed:18657508). Interacts with THBS1 (via the C-terminal domain) (PubMed:19004835, PubMed:8550562). Interacts with SIRPA (PubMed:11509594). Interacts with FAS/CD95; interaction may be enhanced by functional activation (PubMed:15917238). Interacts with SIRPG, UBQLN1 and UBQLN2 (PubMed:10549293, PubMed:15383453, PubMed:18657508). May interact with fibrinogen (PubMed:22079249). Interacts with Aedes aegypti neutrophil-stimulating factor 1; the interaction results in inhibition of phagocytosis activity of macrophages (PubMed:39121194)

  • SwissProt ID

    Q08722

  • Gene ID
    961 [NCBI]
  • Synonyms

    CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP

  • Research Field

    Immunology

CD47 Antibody (YA531) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD47 Antibody (YA531) Product Image
    1/16
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • CD47 Antibody (YA531) Product Image
  • Experimental Validation Results for CD47 Antibody (YA531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • Experimental Validation Results for CD47 Antibody (YA531)
  • CD47 Antibody (YA531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD47 Antibody (YA531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD47 Antibody (YA531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD47 Antibody (YA531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD47 Antibody (YA531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD47 Antibody (YA531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using CD47 Antibody (HY-P80065, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD47 Antibody (YA531) Product Image IHC-P
  • CD47 Antibody (YA531) Product Image IHC-P
  • CD47 Antibody (YA531) Product Image IHC-P
  • CD47 Antibody (YA531) Product Image IHC-P
  • CD47 Antibody (YA531) Product Image IHC-P
  • CD47 Antibody (YA531) Product Image IHC-P