Antimicrobial agent-58
Antimicrobial agent-58 is a potent antimicrobial agent with an MIC of 15.625 μg/mL against B. cereus and S. typhimurium. Antimicrobial agent-58 is an inhibitor of DNA gyrase A and Efflux pump proteins. Antimicrobial agent-58 achieves a dual effect of antimicrobial activity and reversal of multidrug resistance by targeting and inhibiting DNA gyrase A and dually blocking the efflux pump system at both the gene and protein levels. Antimicrobial agent-58 can be used in research on multidrug-resistant bacterial infections.
For research use only. We do not sell to patients.
- Formula: C18H12Cl3N5O3S3
- Molecular Weight:548.87
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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- Purity & Documentation
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Biological Activity
Description
IC50 & Target
[1]|
DNA Gyrase |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
115.41 μg/mL
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Cytotoxicity against human embryonic kidney HEK-293 cells assessed as reduction in cell viability by MTT assay.
Cytotoxicity against human embryonic kidney HEK-293 cells assessed as reduction in cell viability by MTT assay.
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42594422 |
| WI-38 | IC50 |
56.08 μg/mL
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Cytotoxicity against human embryonic lung fibroblast WI-38 cells assessed as reduction in cell viability by MTT assay.
Cytotoxicity against human embryonic lung fibroblast WI-38 cells assessed as reduction in cell viability by MTT assay.
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42594422 |
In Vitro
Antimicrobial agent-58 (compound 7j) exhibits potent bactericidal activity against Bacillus cereus ATCC 10876 and Salmonella typhimurium ATCC 14028, with an MIC value of 15.625 μg/mL[1].
Antimicrobial agent-58 (1/4-1/2 MIC; 16 h) dose-dependently inhibits biofilm formation in in vitro cultures of P. aeruginosa[1].
Antimicrobial agent-58 (1/4-1/2 MIC; cultured to late logarithmic phase OD600 = 1.5) downregulates efflux pump gene expression in multidrug-resistant MDR-P. aeruginosa[1].
Antimicrobial agent-58 (1-128 μg/mL; 1-5 h) exhibits low cytotoxicity in HEK-293 and WI-38 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MDR Pseudomonas aeruginosa
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Concentration:1/4 MIC, 1/2 MIC
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Incubation Time:grown until the late-log phase (OD600 =1.5)
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Result:Significantly and concentration-dependently downregulated the expression of key efflux pump genes (mexA, mexC, mexE).
Chemical Information
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Molecular Weight 548.87
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Formula C18H12Cl3N5O3S3
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SMILES
O=S(C1=CC(Cl)=CC=C1Cl)(NNC(CSC2=NN=C3SC=C(N32)C4=CC=C(C=C4)Cl)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antimicrobial agent-58 Related Classifications
HY-189406 Related Classifications
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