Lentivirus Solution Buffer

1. Based on the desired concentration factor, add an appropriate amount of lentivirus solution buffer and gently pipette up and down to resuspend the pellet.

Note: For example, if 100 mL of lentiviral supernatant is expected to be concentrated 100-fold, add 1 mL of lentivirus solution buffer.

2. Incubate at room temperature for 4 - 6 h to allow the lentiviral particles to fully release into the solution buffer.

3. Centrifuge at 4°C, 11,200 g (12,000 rpm) for 3 min and collect the supernatant. Aliquot appropriately and store at -80°C.

Note: The above centrifugation is based on a small centrifuge (effective radius ~ 7 cm) and ≤ 2 mL centrifuge tubes. For larger volumes, it is recommended to centrifuge in aliquots.

After ultrafiltration of the lentiviral supernatant, add an appropriate amount of lentivirus solution buffer according to experimental requirements, centrifuge to the desired volume, aliquot appropriately, and store at -80°C.