Cat. No.: HY-N2534

Karanjin

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Karanjin is an orally active furanoflavonoid which can be isolated from several Leguminosae. Karanjin exhibits evident anti-diabetic, anti-cancer, anti-inflammatory, antioxidant, anticolitis, anti-ulcer, anti-Alzheimer properties and multiple insect repellent/insecticidal, acaricide properties, suggesting the potential of Karanjin to be applied to relevant research.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 521-88-0
Formula: C₁₈H₁₂O₄
Molecular Weight:292.29

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Karanjin Chemical Structure

CAS No.: 521-88-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All AMPK Isoforms :

All Cytochrome P450 Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	IC₅₀	>100 μM Compound: 5	Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 25466192]

In Vitro

Karanjin (10-25 μM, 16 h) enhances glucose uptake in L6-GLUT4 myc cells through stimulates translocation of GLUT4 to plasma membrane-associated with activation of AMPK pathway and inhibits the activity of PTPase in a dose-dependent manner. Shows no significant effect on cell viability^[1].
Karanjin (4-8 μM, 72 h) blocks the cell cycle of A549 and HL-60 markedly as well as induces significant cell apoptosis^[1].
Karanjin (80, 160 μM, 30 min) decreases ROS level by inhibition of I-κB degradation resulting in restriction of NF-κB nuclear translocation in Hela cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:

A549, HL-60
Concentration:

4, 6, 8 μM for A549, 2, 4, 6 μM for HL-60
Incubation Time:

72 h
Result:

Blocked the cell cycle markedly at G2/M phase of A549 (>60% for 8 μM) and HL-60 (>40% for 6 μM)

In Vivo

Karanjin (50, 100 mg/kg, p.o., 6 h) reduces the blood glucose level of Streptozotocin (HY-13753)-induced diabetic rats^[1].
Karanjin (20 mg/kg/d, p.o., 21 d) reduces joint injury and cartilage damage along with edema and erythema in the AIA model rats^[1].
Karanjin (10, 20 mg/kg/d, p.o., 14 d) significantly decreases serum ACP, ALP and TNFα levels in the AIA model rats ^[1].
Karanjin (25 or 50 mg/kg/d, p.o. or i.g., 8 d) improved learning and memory in Diazepam-induced amnesia mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Streptozotocin-induced diabetic rats^[1]
Dosage:

50, 100 mg/kg
Administration:

Oral gavage (p.o.), wait for 6h
Result:

Lowered the blood glucose level by 11.7% and 20.7% at 50 and 100 mg/kg gavage.

Animal Model:

Adjuvant induced arthritis model (AIA) Wistar strain male albino rats^[1]
Dosage:

10, 20 mg/kg/d
Administration:

Oral gavage (p.o.), 14 d for serum assay, 21 d for histological examination
Result:

Reduced articular cartilage damage, cellular infiltration in the articular cartilage and spongy bone damage significantly. Decreased serum acid phosphatase, alkaline phosphatase levels and TNFα level markedly.

Animal Model:

Diazepam-induced male swiss albino mice ^[1]
Dosage:

25, 50 mg/kg/d
Administration:

Oral gavage (p.o.) or Intraperitoneal injection (i.p.) for 8 d
Result:

Decreased the transfer latency on all the observation days, significantly reversed diazepam-induced amnesia, indicating improved learning and memory in treated mice.

Chemical Information

CAS No. 521-88-0
Appearance Solid
Molecular Weight 292.29
Formula C₁₈H₁₂O₄
Color White to off-white
SMILES

O=C1C2=CC=C(OC=C3)C3=C2OC(C4=CC=CC=C4)=C1OC
Structure Classification
- Flavonoids
- Flavonols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (2)

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Journal Impact Factor

Phytomedicine

Karanjin counteracts OVX-induced bone loss by dual regulating bone remodeling. [Abstract]

2025 Oct:146:157099. PMID: 40774011
Chem Biol Drug Des

The protective effect of karanjin against sepsis-induced acute lung injury in mice is involved in the suppression of the TLR4 pathway. [Abstract]

2024 Jul;104(1):e14579. PMID: 39013775

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (85.53 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4213 mL

17.1063 mL

34.2126 mL

5 mM

0.6843 mL

3.4213 mL

6.8425 mL

10 mM

0.3421 mL

1.7106 mL

3.4213 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Singh A, et al. Karanjin. Phytochemistry. 2021，183:112641. [Content Brief]

[2]. Jaiswal N, et al.Karanjin from Pongamia pinnata induces GLUT4 translocation in skeletal muscle cells in a phosphatidylinositol-3-kinase-independent manner. Eur J Pharmacol. 2011 Nov 16;670(1):22-8. [Content Brief]

[3]. Guo JR, et al. Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancercells. Biol Res. 2015 Jul 26;48:40. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4213 mL	17.1063 mL	34.2126 mL	85.5315 mL
	5 mM	0.6843 mL	3.4213 mL	6.8425 mL	17.1063 mL
	10 mM	0.3421 mL	1.7106 mL	3.4213 mL	8.5531 mL
	15 mM	0.2281 mL	1.1404 mL	2.2808 mL	5.7021 mL
	20 mM	0.1711 mL	0.8553 mL	1.7106 mL	4.2766 mL
	25 mM	0.1369 mL	0.6843 mL	1.3685 mL	3.4213 mL
	30 mM	0.1140 mL	0.5702 mL	1.1404 mL	2.8510 mL
	40 mM	0.0855 mL	0.4277 mL	0.8553 mL	2.1383 mL
	50 mM	0.0684 mL	0.3421 mL	0.6843 mL	1.7106 mL
	60 mM	0.0570 mL	0.2851 mL	0.5702 mL	1.4255 mL
	80 mM	0.0428 mL	0.2138 mL	0.4277 mL	1.0691 mL

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Karanjin

Apoptosis Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

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Anti-diabetic Compound Library

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Pyroptosis Compound Library

Cytoskeleton Compound Library

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Traditional Chinese Medicine Active Compound Library

Flavonoids Library

Anti-Alzheimer's Disease Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Phosphatase Inhibitor Library

Anti-Cancer Metabolism Compound Library

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Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

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Diarrhea-Related Traditional Chinese Medicine Active Compound Library

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Tonifying Traditional Chinese Medicine Monomer Compound Library

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Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Karanjin

All AMPK Isoforms

All Cytochrome P450 Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References