Cat. No.: HY-15449

Kaempferide

(Synonyms: Kaempferol 4'-O-methyl ether)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Kaempferide is an orally active flavonol isolated from Hippophae rhamnoides L. Kaempferide has anticancer, anti-inflammatory, antioxidant, antidiabetic, antiobesity, antihypertensive, and neuroprotective activities. Kaempferide induces apoptosis. Kaempferide promotes osteogenesis through antioxidants and can be used in osteoporosis research.

For research use only. We do not sell to patients.

Purity: 99.80%
CAS No.: 491-54-3
Formula: C₁₆H₁₂O₆
Molecular Weight:300.26

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Kaempferide Chemical Structure

CAS No.: 491-54-3

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Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Estrogen Receptor/ERR Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All GSK-3 Isoforms :

All FOXO Isoforms :

FOXO1
FOXO3

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	2.2 x 10^-5 M Compound: 13	Cytotoxicity against human A549 cells after 72 hrs by XTT assay Cytotoxicity against human A549 cells after 72 hrs by XTT assay	[PMID: 20619940]
B16	IC₅₀	16 μM Compound: 5	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs	[PMID: 19615910]
B16-4A5	IC₅₀	>100 μM Compound: 5	Inhibition of theophylline-stimulated mouse B16-4A5 cell proliferation assessed as cell viability after 68 hrs by WST8 assay Inhibition of theophylline-stimulated mouse B16-4A5 cell proliferation assessed as cell viability after 68 hrs by WST8 assay	[PMID: 19615910]
HeLa	IC₅₀	2 μg/mL Compound: 7	Antiallergic activity in Ca(2+)-stimulated differentiated human HeLa cells assessed as inhibition of cys-leukotriene release after 6 days by ELISA Antiallergic activity in Ca(2+)-stimulated differentiated human HeLa cells assessed as inhibition of cys-leukotriene release after 6 days by ELISA	[PMID: 19942440]
HeLa	IC₅₀	2.3 x 10^-5 M Compound: 13	Cytotoxicity against human HeLa cells after 72 hrs by XTT assay Cytotoxicity against human HeLa cells after 72 hrs by XTT assay	[PMID: 20619940]
HT-1080	ED₅₀	<8 μg/mL Compound: Kaempferide	Cytotoxicity against human HT1080 cells by MTT assay Cytotoxicity against human HT1080 cells by MTT assay	[PMID: 11000036]
HT-1080	ED₅₀	2.91 μg/mL Compound: 20	Cytotoxicity against human HT1080 cells after 24 hrs by MTT assay Cytotoxicity against human HT1080 cells after 24 hrs by MTT assay	[PMID: 9677271]
HT-29	IC₅₀	3.2 x 10^-5 M Compound: 13	Cytotoxicity against human HT-29 cells after 72 hrs by XTT assay Cytotoxicity against human HT-29 cells after 72 hrs by XTT assay	[PMID: 20619940]
Huh-7	CC₅₀	>50 μM Compound: 30	Cytotoxicity against human Huh7.5.1 cells by MTT assay Cytotoxicity against human Huh7.5.1 cells by MTT assay	[PMID: 22445328]
KB	ED₅₀	20 μg/mL Compound: NSC-407294	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
L929	EC₅₀	129 μM Compound: kaempferide	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining	[PMID: 9287415]
L929	EC₅₀	140 μM Compound: kaempferide	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay	[PMID: 9287415]
Monocyte	IC₅₀	5.7 μM Compound: kaempferide	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
Monocyte	IC₅₀	5.7 x 10^-6 M Compound: kaempferide	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]

In Vitro

Kaempferid is toxic in HCC cell lines (HepG2:IC₅₀ = 27.94 µM; Huh7: IC₅₀ = 25.65 µM; N1S1: IC₅₀ = 15.18 µM)^[1].
Kaempferid (5, 20 μM; 48 h) reduces lipid accumulation and oxidative stress in HepG2 cells induced by oral acid (OA) (0.5 mM) (HY-N1446)^[2].
Kaempferide promotes osteogenesis through the FoxO1/β-catenin signaling pathway ^[3]. Kaempferide (10-15 μM; 24 h) is toxic in HeLa cells with an IC₅₀ of 16 μM and can induce apoptosis^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

HepG2
Concentration:

5 μM, 10 μM, 20 μM . Before treatment with OA (HY-N1446) (0.5 mM; 48 h)
Incubation Time:

48 h
Result:

Lowered the expression of proteins related to fat production, including sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS), and stearoyl-CoA desaturase 1 (SCD-1).
Reduced the expression of two adipogenic transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT enhancer-binding protein β (C/EBPβ).
Enhanced the expression of heme oxygenase-1 (HO-1) and nuclear factor erythroid 2-related factor 2 (Nrf2).

In Vivo

Kaempferid (25 mg/kg; IV; three times a week) has anticancer activity in SD (Sprague Dawley) rats^[1]. Kaempferide (10 mg/kg; supplemented in daily diet, once daily for16 weeks) ameliorates oxidative stress and inflammation in obese C57BL/6J mice by inhibiting the TLR4/i-κBα/NF-κB pathway and can alleviate Obesity and glucose and lipid metabolism disorders^[4].
Kaempferide (0.1-0.3 mg/kg; injection, single dose) alleviates myocardial ischemia/reperfusion injury by activating PI3K/Akt/GSK-3β pathway in Sprague Dawley (I/R)-induced rats^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

High-fat diet male C57BL/6J mice model^[4]

Dosage:

10 mg/kg

Administration:

Supplemented in daily diet, once daily for16 weeks

Result:

Reduced the weight, organ weight, and index of mice.
Lowered the levels of glycolipids in mouse serum.
Decreased the expression levels of inflammatory-related genes, including NF-κB, IL-6, ICAM-1, VCAM-1, and TNF-α.

Animal Model:

Ischemia/Reperfusion (I/R) SD rat model^[5].

Dosage:

0.1 mg/kg, 0.3 mg/kg, 3 mg/kg

Administration:

Injection, Single dose. Before the I/R injury induced by Coronary Artery Ligation (CAL) in SD rats.

Result:

Significantly improved heart function, reduced myocardial injury by reducing myocardial enzyme levels, and dose-dependently reduced the area of myocardial infarction in rats.
Significantly decreased serum levels of TNF-α, IL-6, C-reactive protein (CRP), MDA, and ROS, while increasing serum levels of SOD.
Downregulated the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and cleaved caspase-3, and upregulated the phosphorylation expression levels of phospho-Akt (p-Akt) and phospho-glycogen synthase kinase-3β (p-GSK-3β).

Chemical Information

CAS No. 491-54-3

Appearance Solid

Molecular Weight 300.26

Formula C₁₆H₁₂O₆

Color Light yellow to yellow

SMILES

O=C1C(O)=C(C2=CC=C(OC)C=C2)OC3=CC(O)=CC(O)=C13

Synonyms

Kaempferol 4'-O-methyl ether

Structure Classification

Flavonoids

Flavonols

Phenols

Polyphenols

Initial Source

Plants

Zingiberaceae

Alpinia officinarum Hance

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder -20°C 3 years

4°C 2 years

In solvent -80°C 2 years

-20°C 1 year

Publications (6)

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Journal Impact Factor

Most Recent

Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186

Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060

Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530

Ind Crops Prod

2025 Dec 11;239:122458.

Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Evid Based Complement Alternat Med

Identifying Active Compounds and Mechanism of Camellia nitidissima Chi on Anti-Colon Cancer by Network Pharmacology and Experimental Validation. [Abstract]

2021 Aug 26:2021:7169211. PMID: 34484402

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (66.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3304 mL

16.6522 mL

33.3045 mL

5 mM

0.6661 mL

3.3304 mL

6.6609 mL

10 mM

0.3330 mL

1.6652 mL

3.3304 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:

To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2 mg/mL (6.66 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg

Animal Weight (Per Animal) Animal Weight (Per Animal)
g

Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL

Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

%
+

+
%
Tween-80 + +

%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact
MedChemExpress (MCE) .

Tel: 609-228-6898
E-mail: [email protected]
Technical Support: [email protected]

Method for preparing in vivo working solution for animal experiments:
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Saline .

Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)

SDS (396 KB)

English - EN (396 KB)

Français - FR (396 KB)

Deutsch - DE (396 KB)

Norwegian - NO (396 KB)

Español - ES (396 KB)

Swedish - SV (396 KB)

Italian - IT (396 KB)

Portuguese - PT (396 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

References

[1]. Chandrababu G, et al. Kaempferide exhibits an anticancer effect against hepatocellular carcinoma in vitro and in vivo[J]. Naunyn-Schmiedeberg's Archives of Pharmacology, 2023: 1-7.

[2]. Tie F, et al. Kaempferol and kaempferide attenuate oleic acid-induced lipid accumulation and oxidative stress in HepG2 cells[J]. International Journal of Molecular Sciences, 2021, 22(16): 8847. [Content Brief]

[3]. Ma X, et al. Kaempferide enhances antioxidant capacity to promote osteogenesis through FoxO1/β-catenin signaling pathway[J]. European Journal of Pharmacology, 2021, 911: 174555. [Content Brief]

[4]. Tang H, et al. Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice[J]. Iranian Journal of Basic Medical Sciences, 2021, 24(4): 493. [Content Brief]

[5]. Wang D, et al. Kaempferide Protects against Myocardial Ischemia/Reperfusion Injury through Activation of the PI3K/Akt/GSK-3β Pathway. Mediators Inflamm. 2017;2017:5278218. [Content Brief]

[6]. Nath L R, et al. Kaempferide, the most active among the four flavonoids isolated and characterized from Chromolaena odorata, induces apoptosis in cervical cancer cells while being pharmacologically safe[J]. RSC advances, 2015, 5(122): 100912-100922.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

DMSO 1 mM 3.3304 mL 16.6522 mL 33.3045 mL 83.2612 mL

5 mM 0.6661 mL 3.3304 mL 6.6609 mL 16.6522 mL

10 mM 0.3330 mL 1.6652 mL 3.3304 mL 8.3261 mL

15 mM 0.2220 mL 1.1101 mL 2.2203 mL 5.5507 mL

20 mM 0.1665 mL 0.8326 mL 1.6652 mL 4.1631 mL

25 mM 0.1332 mL 0.6661 mL 1.3322 mL 3.3304 mL

30 mM 0.1110 mL 0.5551 mL 1.1101 mL 2.7754 mL

40 mM 0.0833 mL 0.4163 mL 0.8326 mL 2.0815 mL

50 mM 0.0666 mL 0.3330 mL 0.6661 mL 1.6652 mL

60 mM 0.0555 mL 0.2775 mL 0.5551 mL 1.3877 mL

Kaempferide Related Classifications

Cat. No.: HY-15449

Vitamin D Related/Nuclear Receptor Autophagy Anti-infection Apoptosis PI3K/Akt/mTOR Stem Cell/Wnt Metabolic Enzyme/Protease

Estrogen Receptor/ERR Autophagy Bacterial Influenza Virus Apoptosis PI3K Akt GSK-3 FOXO β-catenin

Disease Areas

Cancer Infection Neurological, Eye or Ear Disease Inflammation or Immune System Disease Blood or Cardio-cerebrovascular Disease Metabolic or Endocrine Disease Digestive System Disease Urogenital Disease

Cardiovascular Disease Lipid Metabolism Breast Cancer Pancreatic Cancer Digestive System Cancer Ovarian Cancer Urogenital Cancer Viral Infection Bacterial Infection Depression Pain Digestive System Inflammation Glucose Metabolism

Hypertension Obesity

Natural Products

Disease Research Fields Plants Flavonoids Phenols

Zingiberaceae Infection Flavonols Polyphenols

Alpinia officinarum Hance

Lipids

Polyketides

Reference Standards

Natural Product Standards

Flavonoids Phenols

Neurological Disease Metabolic Disease Inflammation/Immunology Infection Cardiovascular Disease Cancer

Cancer Targeted Therapy Cancer Immunotherapy Cancer Stem Cells Cancer Metabolism and Metastasis

Powered by Bioz See more details on Bioz

Keywords

Kaempferide
491-54-3
Kaempferol 4'-O-methyl ether
Estrogen Receptor/ERR
Autophagy
Bacterial
Influenza Virus
Apoptosis
PI3K
Akt
GSK-3
FOXO
β-catenin
HCC
osteoporosis
osteogenesis
antioxidant
non-alcoholic fatty liver disease (NAFLD)
HepG2
HeLa
Huh7
N1S1
apoptosis
diabetes
obesity
hypertension
neuroprotection
SD (Sprague Dawley)
C57BL /6J
myocardial ischemia/reperfusion injury
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3304 mL	16.6522 mL	33.3045 mL	83.2612 mL
5 mM	0.6661 mL	3.3304 mL	6.6609 mL	16.6522 mL
10 mM	0.3330 mL	1.6652 mL	3.3304 mL	8.3261 mL
15 mM	0.2220 mL	1.1101 mL	2.2203 mL	5.5507 mL
20 mM	0.1665 mL	0.8326 mL	1.6652 mL	4.1631 mL
25 mM	0.1332 mL	0.6661 mL	1.3322 mL	3.3304 mL
30 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7754 mL
40 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0815 mL
50 mM	0.0666 mL	0.3330 mL	0.6661 mL	1.6652 mL
60 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3877 mL

Kaempferide

6 Cited Publications

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Kaempferide

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Publications Citing Use of MedChemExpress (MCE) Kaempferide

All Estrogen Receptor/ERR Isoforms

All PI3K Isoforms