2. Autophagy
  3. Atg7 Autophagy
  4. ATG7-IN-2

ATG7-IN-2 (compound 1) is a potent ATG7 inhibitor, with an IC50 of 0.089 μM. ATG7-IN-2 inhibits autophagy marker LC3B.

For research use only. We do not sell to patients.

ATG7-IN-2 Chemical Structure

ATG7-IN-2 Chemical Structure

CAS No. : 2226227-75-2

Size Price Stock Quantity
5 mg USD 480 In-stock
10 mg USD 800 In-stock
25 mg USD 1650 In-stock
50 mg USD 2600 In-stock
100 mg USD 4000 In-stock
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Based on 1 publication(s) in Google Scholar

ATG7-IN-2 Related Antibodies

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Description

ATG7-IN-2 (compound 1) is a potent ATG7 inhibitor, with an IC50 of 0.089 μM. ATG7-IN-2 inhibits autophagy marker LC3B[1].

IC50 & Target

IC50: 0.089 ± 0.036 μM (ATG7), 0.335 ± 0.049 μM (Thioester), 2.6 μM (LC3B)[1]
Molecular Weight

376.35

Formula

C11H16N6O7S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@](O[C@@H]([C@H]1O)COS(N)(=O)=O)([H])N2C3=NC=NC(N)=C3C(OC)=N2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (265.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 0.5 mg/mL (1.33 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6571 mL 13.2855 mL 26.5710 mL
5 mM 0.5314 mL 2.6571 mL 5.3142 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  Saline

    Solubility: 25 mg/mL (66.43 mM); Clear solution; Need ultrasonic and adjust pH to 1 with 1M HCl

  • Protocol 2

    Add each solvent one by one:  20% HP-β-CD in Saline

    Solubility: 25 mg/mL (66.43 mM); Clear solution; Need ultrasonic and adjust pH to 4 with 1M HCl

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.6571 mL 13.2855 mL 26.5710 mL 66.4275 mL
DMSO 5 mM 0.5314 mL 2.6571 mL 5.3142 mL 13.2855 mL
10 mM 0.2657 mL 1.3286 mL 2.6571 mL 6.6428 mL
15 mM 0.1771 mL 0.8857 mL 1.7714 mL 4.4285 mL
20 mM 0.1329 mL 0.6643 mL 1.3286 mL 3.3214 mL
25 mM 0.1063 mL 0.5314 mL 1.0628 mL 2.6571 mL
30 mM 0.0886 mL 0.4429 mL 0.8857 mL 2.2143 mL
40 mM 0.0664 mL 0.3321 mL 0.6643 mL 1.6607 mL
50 mM 0.0531 mL 0.2657 mL 0.5314 mL 1.3286 mL
60 mM 0.0443 mL 0.2214 mL 0.4429 mL 1.1071 mL
80 mM 0.0332 mL 0.1661 mL 0.3321 mL 0.8303 mL
100 mM 0.0266 mL 0.1329 mL 0.2657 mL 0.6643 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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ATG7-IN-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ATG7-IN-22226227-75-2Atg7AutophagyE1 enzymeautophagyH1650 cellsH4 cellsneurogliomaLC3BNBR1Inhibitorinhibitorinhibit

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