ATUX-1215
Based on 1 Customer Validation
ATUX-1215 is an activator of protein phosphatase 2A (PP2A). ATUX-1215 reduced the phosphorylation of ERK, p38, JNK, and Akt and the secretion of IL-12p70, GM-CSF, and IL1α in Bleomycin hydrochloride (HY-17565A)-treated animals. ATUX-1215 can slow the progression of lung fibrosis.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.94%
- No. CAS: 2910929-53-0
- Fòrmula: C27H24F5NO4S
- Peso molecular:553.54
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
In Vitro
ATUX-1215 (1 μM, 24 h) suppresses TGF-β-induced gene expression of ACTA2, COL1A1, COL1A2 and FN1[1].
ATUX-1215 (0-20 μM, 24 h) inhibits the cell viability of medulloblastoma cells D425 and D341 with LD50s of 10.22 μM and 5.06 μM. ATUX-1215 inhibits the cell migration and invasion, and induces apoptosis in D425 and D341[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:D425 and D341
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Concentration:0-5 μM
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Incubation Time:30 min
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Result:Reduced the expression of PARP, increased the level of cleaved-caspase 9.
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Cell Line:Human lung fibroblasts
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Concentration:1 μM
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Incubation Time:24 h
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Result:Downregulated TGF-β-induced gene expression of ACTA2, COL1A1, COL1A2 and FN1.
In Vivo
ATUX-1215 (75 mg/kg, po, twice daily for 15 days) exhibits antitumor activity in D425Luc-or D341Luc-xenograft mouse models[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Bleomycin-induced mouse pulmonary fibrosis models[1]
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Dosage:5 mg/kg
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Administration:po, once daily for 23 days
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Result:Improved lung function, reduced collagen deposition, inhibited Acta2, Ccn2 and Fn1 gene expression. Inhibited the secretion of IL-12p70, GM-CSF and IL-1α in the bronchoalveolar fluid.
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Animal Model:D425Luc-or D341Luc-xenograft mouse models[2]
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Dosage:75 mg/kg
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Administration:po, twice daily for 15 days
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Result:Prolonged the survival time, reduced and the tumor burden.
Chemical Information
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No. CAS 2910929-53-0
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Appearance Solid
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Peso molecular 553.54
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Fòrmula C27H24F5NO4S
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Color White to off-white
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SMILES
O=S(NC1CC(OCC1)C2C3=CC=C(F)C=C3CCC4=CC(F)=CC=C42)(C5=CC=C(C=C5)OC(F)(F)F)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (180.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.8066 mL | 9.0328 mL | 18.0655 mL | 45.1639 mL |
| 5 mM | 0.3613 mL | 1.8066 mL | 3.6131 mL | 9.0328 mL | |
| 10 mM | 0.1807 mL | 0.9033 mL | 1.8066 mL | 4.5164 mL | |
| 15 mM | 0.1204 mL | 0.6022 mL | 1.2044 mL | 3.0109 mL | |
| 20 mM | 0.0903 mL | 0.4516 mL | 0.9033 mL | 2.2582 mL | |
| 25 mM | 0.0723 mL | 0.3613 mL | 0.7226 mL | 1.8066 mL | |
| 30 mM | 0.0602 mL | 0.3011 mL | 0.6022 mL | 1.5055 mL | |
| 40 mM | 0.0452 mL | 0.2258 mL | 0.4516 mL | 1.1291 mL | |
| 50 mM | 0.0361 mL | 0.1807 mL | 0.3613 mL | 0.9033 mL | |
| 60 mM | 0.0301 mL | 0.1505 mL | 0.3011 mL | 0.7527 mL | |
| 80 mM | 0.0226 mL | 0.1129 mL | 0.2258 mL | 0.5645 mL | |
| 100 mM | 0.0181 mL | 0.0903 mL | 0.1807 mL | 0.4516 mL |
ATUX-1215 Related Classifications
HY-156420 Related Classifications
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