BeRST voltage sensitive dye
BeRST voltage sensitive dye is a silicon-rhodamine-based voltage-sensitive dye that serves as a membrane potential indicator, featuring fast response kinetics, high sensitivity, photostability, and excellent signal-to-noise ratio. BeRST voltage sensitive dye relies on the photoinduced electron transfer (PeT) mechanism to report changes in neuronal membrane potential via fluorescent signals. BeRST voltage sensitive dye localizes to the extracellular surface of neuronal plasma membranes, including cell bodies and neurites. BeRST voltage sensitive dye can be used in studies related to Alzheimer's disease (Ex/Em = 658/683 nm).
For research use only. We do not sell to patients.
- Formula: C45H51N3O4SSi
- Molecular Weight:758.05
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
BeRST voltage sensitive dye (BeRST 1) (0.1-1 µM; 20 min loading, 7 days chronic amyloid beta exposure) enables high-fidelity, high-throughput optical recording of action potentials and network connectivity in dissociated rat hippocampal neurons, with near-perfect correlation to patch-clamp electrophysiology, and reliably detects changes in neuronal activity and connectivity following pharmacological inhibition, chronic amyloid beta 1-42 exposure, and developmental maturation[1].
BeRST voltage sensitive dye (500 nM; 30 min) enables high-temporal-resolution voltage imaging of dissociated 14-16 DIV rat hippocampal neurons, detecting action potentials in single trials and revealing that β-hydroxybutyrate treatment significantly reduces neuronal firing frequency, particularly in cells with lower intrinsic firing rates[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 758.05
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Formula C45H51N3O4SSi
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SMILES
COC1=C(/C=C/C2=CC=C(/C=C/C3=CC=C(/C(C4=CC=C(N(C)C)C=C4[Si](C)(C)C)=C5C=C/C(C=C/5)=[N+](C)/C)C(S(=O)([O-])=O)=C3)C=C2)C=CC(N(C)C)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Walker AS, et al. Optical Spike Detection and Connectivity Analysis With a Far-Red Voltage-Sensitive Fluorophore Reveals Changes to Network Connectivity in Development and Disease. Frontiers in neuroscience. 2021;15:643859. [Content Brief]
[2]. Kirk MJ, et al. Monitoring neuronal activity with voltage-sensitive fluorophores. Methods in enzymology. 2020;640:185-204. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BeRST voltage sensitive dye Related Classifications
HY-D3480 Related Classifications
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