Biotin-PEG5-NHS ester

Name: Biotin-PEG5-NHS ester
Brand: MedChemExpress
SKU: HY-174892
Rating: 4.5 (1 reviews)

Cat. No.: HY-174892 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Biotin-PEG5-NHS ester is a heterobifunctional crosslinker as well as a polyethylene glycol-containing bioconjugation reagent. Biotin-PEG5-NHS ester is widely used in scientific research fields such as the construction of highly sensitive biosensors, the development of targeted drug delivery systems (e.g., liposome modification), immunofluorescence imaging, and protein affinity purification, and serves as an important tool for achieving precise labeling and functionalization of biomolecules.

For research use only. We do not sell to patients.

Biotin-PEG5-NHS ester Chemical Structure

Size	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Biotin-PEG5-NHS ester:

Biotin-PEG32-NHS ester Get quote
Biotin-PEG20-NHS ester Get quote

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•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Biotin-PEG5-NHS ester is a highly efficient bioconjugation reagent, mainly used for the specific introduction of biotin tags onto molecules containing primary amine groups (-NH₂) (such as proteins, antibodies, polypeptides) or material surfaces. The NHS ester group in its molecular structure rapidly reacts with amine groups under mild conditions to form stable amide bonds, while the intermediate PEG5 chain (pentaethylene glycol) acts as a flexible spacer arm to increase the spatial freedom of the molecule, and can also significantly improve water solubility and reduce non-specific adsorption.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

632.72

Formula

C₂₇H₄₄N₄O₁₁S

Appearance

Oil

Color

White to off-white

SMILES

[H][C@]12NC(N[C@]1(CS[C@H]2CCCCC(NCCOCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O)=O)[H])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (158.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5805 mL	7.9024 mL	15.8048 mL
5 mM	0.3161 mL	1.5805 mL	3.1610 mL
10 mM	0.1580 mL	0.7902 mL	1.5805 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (266 KB) SDS (251 KB)

COA (242 KB) HNMR (340 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Ohkawa Y, et al. Detailed protocol─ Chromatin Integration labeling[J]. 2018.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5805 mL	7.9024 mL	15.8048 mL	39.5120 mL
	5 mM	0.3161 mL	1.5805 mL	3.1610 mL	7.9024 mL
	10 mM	0.1580 mL	0.7902 mL	1.5805 mL	3.9512 mL
	15 mM	0.1054 mL	0.5268 mL	1.0537 mL	2.6341 mL
	20 mM	0.0790 mL	0.3951 mL	0.7902 mL	1.9756 mL
	25 mM	0.0632 mL	0.3161 mL	0.6322 mL	1.5805 mL
	30 mM	0.0527 mL	0.2634 mL	0.5268 mL	1.3171 mL
	40 mM	0.0395 mL	0.1976 mL	0.3951 mL	0.9878 mL
	50 mM	0.0316 mL	0.1580 mL	0.3161 mL	0.7902 mL
	60 mM	0.0263 mL	0.1317 mL	0.2634 mL	0.6585 mL
	80 mM	0.0198 mL	0.0988 mL	0.1976 mL	0.4939 mL
	100 mM	0.0158 mL	0.0790 mL	0.1580 mL	0.3951 mL