Biotin-PEG5-NHS ester
Based on 1 Customer Validation
Biotin-PEG5-NHS ester is a heterobifunctional crosslinker as well as a polyethylene glycol-containing bioconjugation reagent. Biotin-PEG5-NHS ester is widely used in scientific research fields such as the construction of highly sensitive biosensors, the development of targeted drug delivery systems (e.g., liposome modification), immunofluorescence imaging, and protein affinity purification, and serves as an important tool for achieving precise labeling and functionalization of biomolecules.
For research use only. We do not sell to patients.
- Purity: 98.0%
- Formula: C27H44N4O11S
- Molecular Weight:632.72
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Biotin-PEG5-NHS ester is a highly efficient bioconjugation reagent, mainly used for the specific introduction of biotin tags onto molecules containing primary amine groups (-NH2) (such as proteins, antibodies, polypeptides) or material surfaces. The NHS ester group in its molecular structure rapidly reacts with amine groups under mild conditions to form stable amide bonds, while the intermediate PEG5 chain (pentaethylene glycol) acts as a flexible spacer arm to increase the spatial freedom of the molecule, and can also significantly improve water solubility and reduce non-specific adsorption.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Oil
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Molecular Weight 632.72
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Formula C27H44N4O11S
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Color White to off-white
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SMILES
[H][C@]12NC(N[C@]1(CS[C@H]2CCCCC(NCCOCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O)=O)[H])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (158.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5805 mL | 7.9024 mL | 15.8048 mL | 39.5120 mL |
| 5 mM | 0.3161 mL | 1.5805 mL | 3.1610 mL | 7.9024 mL | |
| 10 mM | 0.1580 mL | 0.7902 mL | 1.5805 mL | 3.9512 mL | |
| 15 mM | 0.1054 mL | 0.5268 mL | 1.0537 mL | 2.6341 mL | |
| 20 mM | 0.0790 mL | 0.3951 mL | 0.7902 mL | 1.9756 mL | |
| 25 mM | 0.0632 mL | 0.3161 mL | 0.6322 mL | 1.5805 mL | |
| 30 mM | 0.0527 mL | 0.2634 mL | 0.5268 mL | 1.3171 mL | |
| 40 mM | 0.0395 mL | 0.1976 mL | 0.3951 mL | 0.9878 mL | |
| 50 mM | 0.0316 mL | 0.1580 mL | 0.3161 mL | 0.7902 mL | |
| 60 mM | 0.0263 mL | 0.1317 mL | 0.2634 mL | 0.6585 mL | |
| 80 mM | 0.0198 mL | 0.0988 mL | 0.1976 mL | 0.4939 mL | |
| 100 mM | 0.0158 mL | 0.0790 mL | 0.1580 mL | 0.3951 mL |
Biotin-PEG5-NHS ester Related Classifications
HY-174892 Related Classifications
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