BODIPY-FL
Based on 1 Customer Validation
BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm.
For research use only. We do not sell to patients.
- Purity: 99.15%
- CAS No.: 165599-63-3
- Formula: C14H15BF2N2O2
- Molecular Weight:292.09
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | EC50 |
>30 μM
Compound: 7; BODIPY FL
|
Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
|
[PMID: 31718946] |
| A-375 | EC50 |
>30 μM
Compound: 7; BODIPY FL
|
Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
|
[PMID: 31718946] |
| FaDu | EC50 |
>30 μM
Compound: 7; BODIPY FL
|
Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
|
[PMID: 31718946] |
| HT-29 | EC50 |
>30 μM
Compound: 7; BODIPY FL
|
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
|
[PMID: 31718946] |
| MCF7 | EC50 |
>30 μM
Compound: 7; BODIPY FL
|
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
|
[PMID: 31718946] |
| NIH3T3 | EC50 |
>30 μM
Compound: 7; BODIPY FL
|
Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
|
[PMID: 31718946] |
BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells[3].
BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
516
495
Chemical Information
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CAS No. 165599-63-3
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Appearance Solid
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Molecular Weight 292.09
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Formula C14H15BF2N2O2
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Color Brown to red
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SMILES
O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (342.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Leung K. BODIPY-FL-neutravidin-biotin-Cetuximab. 2008 Jan 15 [updated 2008 Feb 25]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. PMID: 20641313. [Content Brief]
[2]. Kurata S, et al. Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY((R)) FL-labeled probe or primer. Nucleic Acids Res. 2001 Mar 15;29(6):E34. [Content Brief]
[3]. Fan W, et al. Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells. Bio Protoc. 2022 Nov 20;12(22):e4555. [Content Brief]
[4]. Guseva GB, et al. Design, Spectral Characteristics, and Possibilities for Practical Application of BODIPY FL-Labeled Monoterpenoid. ACS Appl Bio Mater. 2021 Aug 16;4(8):6227-6235. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4236 mL | 17.1180 mL | 34.2360 mL | 85.5901 mL |
| 5 mM | 0.6847 mL | 3.4236 mL | 6.8472 mL | 17.1180 mL | |
| 10 mM | 0.3424 mL | 1.7118 mL | 3.4236 mL | 8.5590 mL | |
| 15 mM | 0.2282 mL | 1.1412 mL | 2.2824 mL | 5.7060 mL | |
| 20 mM | 0.1712 mL | 0.8559 mL | 1.7118 mL | 4.2795 mL | |
| 25 mM | 0.1369 mL | 0.6847 mL | 1.3694 mL | 3.4236 mL | |
| 30 mM | 0.1141 mL | 0.5706 mL | 1.1412 mL | 2.8530 mL | |
| 40 mM | 0.0856 mL | 0.4280 mL | 0.8559 mL | 2.1398 mL | |
| 50 mM | 0.0685 mL | 0.3424 mL | 0.6847 mL | 1.7118 mL | |
| 60 mM | 0.0571 mL | 0.2853 mL | 0.5706 mL | 1.4265 mL | |
| 80 mM | 0.0428 mL | 0.2140 mL | 0.4280 mL | 1.0699 mL | |
| 100 mM | 0.0342 mL | 0.1712 mL | 0.3424 mL | 0.8559 mL |
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