1. Cell Cycle/DNA Damage
  2. CRISPR/Cas9
  3. BRD0539

BRD0539 

Cat. No.: HY-136251
Handling Instructions

BRD0539 is a cell-permeable and non-toxic inhibitor of CRISPR-Cas9. BRD0539 inhibits Streptococcus pyogenes Cas9 (SpCas9) (apparent IC50=22 μM) in an in vitro DNA cleavage assay.

For research use only. We do not sell to patients.

BRD0539 Chemical Structure

BRD0539 Chemical Structure

CAS No. : 1403838-79-8

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BRD0539 is a cell-permeable and non-toxic inhibitor of CRISPR-Cas9. BRD0539 inhibits Streptococcus pyogenes Cas9 (SpCas9) (apparent IC50=22 μM) in an in vitro DNA cleavage assay[1].

IC50 & Target

IC50: 22 μM (SpCas9)[1]

In Vitro

BRD0539 dose-dependently blocks the formation of the DNA-bound state in a dose-dependent fashion. BRD0539 impairs the perturbation induced by the 4PAM DNA[1].
BRD0539 does not interfere with the SpCas9:gRNA interaction[1].
BRD0539 is able to inhibit SpCas9 in the eGFP-disruption assay, BRD0539 is unable to inhibit FnCpf1, a structurally different CRISPR-associated nuclease, in the same assay, further highlighting the specificity of BRD0539[1].

Molecular Weight

452.54

Formula

C₂₅H₂₅FN₂O₃S

CAS No.

1403838-79-8

SMILES

OC[[email protected]@H]1NC2=C(C=C(C3=CC=CC=C3F)C=C2)[[email protected]@]4([H])[[email protected]]1([H])CCN4S(=O)(C5=CC=C(C)C=C5)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

BRD0539BRD 0539BRD-0539CRISPR/Cas9CRISPRCas9genomeeditingtranscriptionactivationS. pyogenesSpCas9Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product name:
BRD0539
Cat. No.:
HY-136251
Quantity:
MCE Japan Authorized Agent: