Brom0phenol blue sodium

Cat. No.: HY-D0011: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bromophenol blue sodium is a pH indicator. It changes from yellow at pH 3.0 to blue at pH 4.6. Bromophenol blue sodium is also used as a tracking dye to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.

For research use only. We do not sell to patients.

Brom0phenol blue sodium Chemical Structure

CAS No. : 62625-28-9

Size	Price	Stock	Quantity
1 g	USD 30	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

691.94

Formula

C₁₉H₉Br₄NaO₅S

CAS No.

62625-28-9

Appearance

Solid

Color

Brown to black

SMILES

O=S1(OC2=CC=CC=C2C1(C3=CC(Br)=C(O)C(Br)=C3)C4=CC(Br)=C(O[Na])C(Br)=C4)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (72.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (14.45 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4452 mL	7.2261 mL	14.4521 mL
5 mM	0.2890 mL	1.4452 mL	2.8904 mL
10 mM	0.1445 mL	0.7226 mL	1.4452 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Select Batch:

Data Sheet (280 KB) SDS (0 KB)

COA (261 KB)

Handling Instructions (2659 KB)

References

[1]. Shokrollahi A, Zare E. Determination of acidity constants of bromophenol blue and phenol red indicators by solution scanometric method and comparison with spectrophotometric results. Journal of Molecular Liquids, 2016, 219: 1165-1171.

[2]. Gish G, Eckstein F. DNA and RNA sequence determination based on phosphorothioate chemistry. Science. 1988 Jun 10;240(4858):1520-2. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.4452 mL	7.2261 mL	14.4521 mL	36.1303 mL
	5 mM	0.2890 mL	1.4452 mL	2.8904 mL	7.2261 mL
	10 mM	0.1445 mL	0.7226 mL	1.4452 mL	3.6130 mL
DMSO	15 mM	0.0963 mL	0.4817 mL	0.9635 mL	2.4087 mL
	20 mM	0.0723 mL	0.3613 mL	0.7226 mL	1.8065 mL
	25 mM	0.0578 mL	0.2890 mL	0.5781 mL	1.4452 mL
	30 mM	0.0482 mL	0.2409 mL	0.4817 mL	1.2043 mL
	40 mM	0.0361 mL	0.1807 mL	0.3613 mL	0.9033 mL
	50 mM	0.0289 mL	0.1445 mL	0.2890 mL	0.7226 mL
	60 mM	0.0241 mL	0.1204 mL	0.2409 mL	0.6022 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.