1. Anti-infection
  2. Bacterial
  3. Buforin II

Buforin II, derived from buforin I, a protein isolated from the stomach of the Asian toad Bufo bufo gargarizans, is a potent antimicrobial peptide. Buforin II has antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Buforin II Chemical Structure

Buforin II Chemical Structure

CAS No. : 172998-24-2

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1 mg USD 380 In-stock
5 mg USD 850 In-stock
10 mg USD 1380 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Buforin II, derived from buforin I, a protein isolated from the stomach of the Asian toad Bufo bufo gargarizans, is a potent antimicrobial peptide. Buforin II has antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria[1].

In Vitro

Buforin II inhibits A. baumanniiATCC 19606 and multiresistant strains with the similar MIC values of 8 mg/L[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Buforin II (intravenously a single dose, 1 mg/kg) has potent antibacterial activity that effectively reduces lethality and leads to a significant reduction in plasma endotoxin and cytokine concentrations in male Wistar rat model of sepsis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats weighing 200 to 300 g with A. baumannii ATCC 19606[1]
Dosage: 1 mg/kg
Administration: Intravenously a single dose
Result: Reduced mortality by 40% in the treatment group and by 20% in the rifampicin (10 mg/kg) combination group.
Reduced TNF, IL-6 and endotoxin plasma levels by 33%, 25% and 32%, respectively.
Animal Model: Male Wistar rats weighing 200 to 300 g with the multiresistant strain[1]
Dosage: 1 mg/kg
Administration: Intravenously a single dose
Result: Reduced mortality by 46.6% in the treatment group and by 20% in the rifampicin (10 mg/kg) combination group.
Reduced TNF, IL-6 and endotoxin plasma levels by 46%, 20% and 28%, respectively.
Molecular Weight

2434.85

Formula

C106H184N40O26

CAS No.
Appearance

Solid

Color

White to off-white

Sequence Shortening

TRSSRAGLQFPVGRVHRLLRK

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (41.07 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4107 mL 2.0535 mL 4.1070 mL
5 mM 0.0821 mL 0.4107 mL 0.8214 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.4107 mL 2.0535 mL 4.1070 mL 10.2676 mL
5 mM 0.0821 mL 0.4107 mL 0.8214 mL 2.0535 mL
10 mM 0.0411 mL 0.2054 mL 0.4107 mL 1.0268 mL
15 mM 0.0274 mL 0.1369 mL 0.2738 mL 0.6845 mL
20 mM 0.0205 mL 0.1027 mL 0.2054 mL 0.5134 mL
25 mM 0.0164 mL 0.0821 mL 0.1643 mL 0.4107 mL
30 mM 0.0137 mL 0.0685 mL 0.1369 mL 0.3423 mL
40 mM 0.0103 mL 0.0513 mL 0.1027 mL 0.2567 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Buforin II Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Buforin II
Cat. No.:
HY-P1630
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