2. Anti-infection
  3. HBV
  4. Bulevirtide

Bulevirtide (Myrcludex B) is a NTCP inhibitor, a linear lipopeptide of 47 amino acids. Bulevirtide inhibits HBV and HDV entry into liver cells, blocks HBV infection in hepatocytes, and participates in HBV transcriptional suppression. Bulevirtide can be used in HDV infection and compensated cirrhosis research.

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Custom Peptide Synthesis

CAS No. : 2012558-47-1

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Based on 7 publication(s) in Google Scholar

Bulevirtide Related Antibodies

Top Publications Citing Use of Products

    Bulevirtide purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2026 Apr 7;14(4):e0263825.

    The inhibitory effect of Myrcludex B (Bulevirtide) on HBV infection was evaluated using an immunospot assay. HepG2-NTCP cells were incubated with Myrcludex B at 37°C for 1 hour, and then infected with 300 HBV gec/cell.

    Bulevirtide purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2025 Sep 15.  [Abstract]

    Quantification of internalized HBV DNA in HepG2-NTCP vector, CDC42-CA, CDC42-DN cells and HepG2-NTCP cells treated with 200 nM Myrcludex B (Bulevirtide) at 8 hpi by qPCR.

    Bulevirtide purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2025 Sep 15.  [Abstract]

    HepG2-NTCP cells were infected with HBV in the presence of increasing concentrations of Myrcludex B (Bulevirtide). HepG2 cells were used as negative control for infection.

    Bulevirtide purchased from MedChemExpress. Usage Cited in: Biomolecules. 2024 Sep 24;14(10):1201.  [Abstract]

    HepG2-NTCP cells were infected with HBV for 4 days in the presence and absence of myrcludex-B (Bulevirtide), and the levels of extracellular HBV DNA, along with intracellular protein levels, were measured.

    Bulevirtide purchased from MedChemExpress. Usage Cited in: Biomolecules. 2024 Sep 24;14(10):1201.  [Abstract]

    HepG2-NTCP cells were infected with HBV for 4 days with or without myrcludex-B (Bulevirtide), and intracellular ROS levels were measured.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Bulevirtide (Myrcludex B) is a NTCP inhibitor, a linear lipopeptide of 47 amino acids. Bulevirtide inhibits HBV and HDV entry into liver cells, blocks HBV infection in hepatocytes, and participates in HBV transcriptional suppression. Bulevirtide can be used in HDV infection and compensated cirrhosis research[1][2].

    In Vitro

    Bulevirtide (200 nM, 24 h) inihibits NTCP through non-covalent binding in a time- and dose-dependent manner, transfer to a newly synthesized NTCP molecule[3].
    Bulevirtide (Huh7-NTCP cells, 2 μM, 9 days) exhibits potential antiviral activity as replication inhibitor, which blocks upregulation of NTCP mediated-HBV replication in Huh7-NTCP cells [5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Bulevirtide Related Antibodies
    In Vivo

    Bulevirtide (2 μg/g, s.c. for everyday) prevents HBV spreading from infected human hepatocytes in uPA/SCID mice, hinders amplification of the cccDNA pool in initially infected hepatocytes[4].
    Bulevirtide (2 μg/g/d, s.c. for 3 weeks) blocks HBV cell entry in uPA/SCID mice through addressing the hepatocyte component rather than affecting virion productivity within the infected hepatocyte or the half-life of these cells[4].
    Bulevirtide (5 μg,s.c., twice a day for 4 days) blocks upregulation of NTCP mediated-HBV replication in C57BL/6 mice as a replication inhibitor[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: HBV infected uPA/SCID mice[3]
    Dosage: 2 μg/g
    Administration: subcutaneous injection, for everyday
    Result: Blocked the viremia and and HBsAg concentrations.
    Maintained the cell death rate and proliferated hepatocytes amount.
    Clinical Trial
    Molecular Weight

    5398.86

    Formula

    C248H355N65O72
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    {Myr}-Gly-Thr-Asn-Leu-Ser-Val-Pro-Asn-Pro-Leu-Gly-Phe-Phe-Pro-Asp-His-Gln-Leu-Asp-Pro-Ala-Phe-Gly-Ala-Asn-Ser-Asn-Asn-Pro-Asp-Trp-Asp-Phe-Asn-Pro-Asn-Lys-Asp-His-Trp-Pro-Glu-Ala-Asn-Lys-Val-Gly-NH2
    Sequence Shortening

    {Myr}-GTNLSVPNPLGFFPDHQLDPAFGANSNNPDWDFNPNKDHWPEANKVG-NH2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    Solvent & Solubility
    In Vitro: 

    H2O : 83.33 mg/mL (15.43 mM; ultrasonic and adjust pH to 8 with NH3·H2O)

    DMSO : 50 mg/mL (9.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.1852 mL 0.9261 mL 1.8522 mL
    5 mM 0.0370 mL 0.1852 mL 0.3704 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (0.46 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (0.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.70%

    SDS (252 KB)

    COA (273 KB) RP-HPLC (251 KB) MS (74 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 0.1852 mL 0.9261 mL 1.8522 mL 4.6306 mL
    5 mM 0.0370 mL 0.1852 mL 0.3704 mL 0.9261 mL
    H2O 10 mM 0.0185 mL 0.0926 mL 0.1852 mL 0.4631 mL
    15 mM 0.0123 mL 0.0617 mL 0.1235 mL 0.3087 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Bulevirtide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bulevirtide2012558-47-1Myrcludex BHBVHepatitis B virusHBV transcriptional suppressionHDV infectioncompensated cirrhosisInhibitorinhibitorinhibit

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