[Sar9,Met(O2)11]-Substance P
Based on 1 Customer Validation
[Sar9,Met(O2)11]-Substance P is a tachykinin NK1 receptor selective agonist.
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- Pureté: 98.65%
- CAS No.: 110880-55-2
- Formule: C64H100N18O15S
- Masse moléculaire:1393.66
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Stockage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
![[Sar9,Met(O2)11]-Substance P Chemical Structure](https://file.medchemexpress.com/product_pic/hy-p1012.gif "Click to View [Sar9,Met(O2)11]-Substance P Chemical Structure")
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
NK1 receptor[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U-373MG ATCC | IC50 |
1.1 x 10-10 M
Compound: [Sar9,Met(O2)11]-SP
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Displacement of [125I]BH-SP from NK1 receptor in human U373MG cells
Displacement of [125I]BH-SP from NK1 receptor in human U373MG cells
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[PMID: 26988801] |
| U-373MG ATCC | IC50 |
1.1 x 10-10 M
Compound: [Sar(9),Met(O2)11]-SP
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Displacement of [125I]BH-SP from NK1 receptor in human U373MG cells measured after 30 mins by scintillation counting method
Displacement of [125I]BH-SP from NK1 receptor in human U373MG cells measured after 30 mins by scintillation counting method
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[PMID: 27876250] |
[Sar9,Met(O2)11]-Substance P and septide (10-100 pmol per rat, i.c.v.) are equipotent in increasing mean arterial blood pressure (MAP) and heart rate (HR), yet they have dissimilar time-course. Both agonists increase dose-dependently face washing and sniffing while [Sar9,Met(O2)11]-Substance P is the sole to produce grooming[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 110880-55-2
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Appearance Solid
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Masse moléculaire 1393.66
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Formule C64H100N18O15S
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Color White to off-white
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Sequence
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-{Sar}-Leu-{Met[O2]}-NH2
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Sequence Shortening
RPKPQQFF-{Sar}-L-{Met[O2]}-NH2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
H2O : 100 mg/mL (71.75 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (71.75 mM); Clear solution; Need ultrasonic
Protocole
Rats initially receive an i.c.v. injection of artificial cerebrospinal fluid (aCSF; 1 μl) followed 60 min later by a single dose of either [Sar9,Met(O2)11]-Substance P (10 pmol (n=9), 25 pmol (n=9), 65 pmol (n=8) or 100 pmol (n=8)) or septide (10 pmol (n=12), 25 pmol (n=9), 65 pmol (n=6) or 100 pmol (n=6)) to construct a complete dose-response curve. Each rat is selected randomly and injected with only one of the two agonists for the remainder of the protocol. Increasing doses of [Sar9,Met(O2)11]-Substance P or septide are given at 24 h intervals on day 1 (10 pmol), day 2 (25 pmol), day 3 (65 pmol) and day 4 (100 pmol). Control rats (n=18) receive only the vehicle (aCSF) each day of experiment. Peptides are administered in a volume of 1 μL of vehicle followed by 5 μL flush volume of aCSF which corresponds to the void volume of the catheter. Each dose is calculated per rat in 1 μL solution[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
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Fiche technique (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.7175 mL | 3.5877 mL | 7.1754 mL | 17.9384 mL |
| 5 mM | 0.1435 mL | 0.7175 mL | 1.4351 mL | 3.5877 mL | |
| 10 mM | 0.0718 mL | 0.3588 mL | 0.7175 mL | 1.7938 mL | |
| 15 mM | 0.0478 mL | 0.2392 mL | 0.4784 mL | 1.1959 mL | |
| 20 mM | 0.0359 mL | 0.1794 mL | 0.3588 mL | 0.8969 mL | |
| 25 mM | 0.0287 mL | 0.1435 mL | 0.2870 mL | 0.7175 mL | |
| 30 mM | 0.0239 mL | 0.1196 mL | 0.2392 mL | 0.5979 mL | |
| 40 mM | 0.0179 mL | 0.0897 mL | 0.1794 mL | 0.4485 mL | |
| 50 mM | 0.0144 mL | 0.0718 mL | 0.1435 mL | 0.3588 mL | |
| 60 mM | 0.0120 mL | 0.0598 mL | 0.1196 mL | 0.2990 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
[Sar9,Met(O2)11]-Substance P Related Classifications
HY-P1012 Related Classifications
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