EBE-A22
Based on 1 publication(s) in Google Scholar
EBE-A22 is a derivative of PD153035, which can inhibit the phosphorylation of ErbB-1, but EBE-A22 does not have this activity.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.86%
- CAS No.: 229476-53-3
- Formule: C17H16BrN3O2
- Masse moléculaire:374.23
-
Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) EBE-A22
MoreVoir tous les produits spécifiques à Isoform EGFR
More
Activité biologique
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
152 nM
Compound: 63
|
Concentration required to inhibit the phosphorylation of a 14-residue fragment of phospholipase C gamma 1 by Epidermal growth factor receptor from A431 cell vesicles
Concentration required to inhibit the phosphorylation of a 14-residue fragment of phospholipase C gamma 1 by Epidermal growth factor receptor from A431 cell vesicles
|
[PMID: 8568816] |
| EA.hy 926 | IC50 |
3.95 μM
Compound: 4a
|
Antiproliferative activity against human EAhy926 cells at 10 uM after 72 hrs by MTS assay
Antiproliferative activity against human EAhy926 cells at 10 uM after 72 hrs by MTS assay
|
[PMID: 21353546] |
| HT-29 | IC50 |
6.1 μM
Compound: 1b, EBE-A22
|
Antiproliferative activity against human HT-29 cells after 72 hrs by MTS assay
Antiproliferative activity against human HT-29 cells after 72 hrs by MTS assay
|
[PMID: 21033670] |
| HT-29 | IC50 |
6.1 μM
Compound: 4a
|
Antiproliferative activity against human HT-29 cells at 10 uM after 72 hrs by MTS assay
Antiproliferative activity against human HT-29 cells at 10 uM after 72 hrs by MTS assay
|
[PMID: 21353546] |
| MCF7 | IC50 |
9.2 μM
Compound: 1b, EBE-A22
|
Antiproliferative activity against human MCF7 cells after 72 hrs by MTS assay
Antiproliferative activity against human MCF7 cells after 72 hrs by MTS assay
|
[PMID: 21033670] |
| MCF7 | IC50 |
9.2 μM
Compound: 4a
|
Antiproliferative activity against human MCF7 cells at 10 uM after 72 hrs by MTS assay
Antiproliferative activity against human MCF7 cells at 10 uM after 72 hrs by MTS assay
|
[PMID: 21353546] |
| PC-3 | IC50 |
>10 μM
Compound: 1b, EBE-A22
|
Antiproliferative activity against human PC3 cells after 72 hrs by MTS assay
Antiproliferative activity against human PC3 cells after 72 hrs by MTS assay
|
[PMID: 21033670] |
Chemical Information
-
CAS No. 229476-53-3
-
Appearance Solid
-
Masse moléculaire 374.23
-
Formule C17H16BrN3O2
-
Color White to off-white
-
SMILES
BrC1=CC(N(C)C2=NC=NC3=CC(OC)=C(OC)C=C23)=CC=C1
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576
Solvant et solubilité
In Vitro:
DMSO : 50 mg/mL (133.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
-
Fiche technique (273 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6722 mL | 13.3608 mL | 26.7215 mL | 66.8038 mL |
| 5 mM | 0.5344 mL | 2.6722 mL | 5.3443 mL | 13.3608 mL | |
| 10 mM | 0.2672 mL | 1.3361 mL | 2.6722 mL | 6.6804 mL | |
| 15 mM | 0.1781 mL | 0.8907 mL | 1.7814 mL | 4.4536 mL | |
| 20 mM | 0.1336 mL | 0.6680 mL | 1.3361 mL | 3.3402 mL | |
| 25 mM | 0.1069 mL | 0.5344 mL | 1.0689 mL | 2.6722 mL | |
| 30 mM | 0.0891 mL | 0.4454 mL | 0.8907 mL | 2.2268 mL | |
| 40 mM | 0.0668 mL | 0.3340 mL | 0.6680 mL | 1.6701 mL | |
| 50 mM | 0.0534 mL | 0.2672 mL | 0.5344 mL | 1.3361 mL | |
| 60 mM | 0.0445 mL | 0.2227 mL | 0.4454 mL | 1.1134 mL | |
| 80 mM | 0.0334 mL | 0.1670 mL | 0.3340 mL | 0.8350 mL | |
| 100 mM | 0.0267 mL | 0.1336 mL | 0.2672 mL | 0.6680 mL |
EBE-A22 Related Classifications
HY-14347 Related Classifications
Powered by Bioz