HS-291
HS-291 is a HtpG inhibitor of Borrelia burgdorferi (Bb). HS-291 contains BX-2819 (high affinity for Bb HtpG), PEG linker, and Verteporfin (HY-B0146) (a photoactive toxin).HS-291 produces reactive oxygen species under light activation to oxidize HtpG and a discrete protein subset near chaperone proteins and can quickly and irreversibly inactivate Bb.
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- Formule: C156H194N18O36S2
- Masse moléculaire:2961.44
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
HS-291 (0.1-10 μM, 24 h) inhibits Bb culture viability when excited with nrIR (Ex: 693nm 5 J/cm2)[1].
HS-291 (10 μM, 90 min) can specifically target Bb in Bb culture[1].
HS-291 (0-10 μM, 24 h) causes cell wall blebbing, nucleoid condensation and loss of cell wall integrity of cells when excited with nrIR (Ex: 693nm 5 J/cm2)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Masse moléculaire 2961.44
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Formule C156H194N18O36S2
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SMILES
O=C(NCCOCCOCCOCCOCCOCCOCCOCCOCCC(NCC1=CC=C(N2C(C3=C(O)C=C(O)C(C(C)C)=C3)=NNC2=S)C=C1)=O)CCC4=C(/C=C5C(CCC(OC)=O)=C(C(/C=C6N/C(C(C)=C\6C=C)=C\C(C7=CC=C(C(OC)=O)[C@@H](C(OC)=O)[C@]7/8C)=NC8=C/9)=N/5)C)NC9=C4C.CC(CCC%10=C%11NC(/C=C([C@@]%12%13C)\N=C(C%12=CC=C([C@H]%13C(OC)=O)C(OC)=O)/C=C(C(C)=C/%14C=C)\NC%14=C/C(C(C)=C/%15CCC(ONCCOCCOCCOCCOCCOCCOCCOCCOCCC(NCC%16=CC=C(C=C%16)N%17C(NN=C%17C%18=C(C=C(C(C(C)C)=C%18)O)O)=S)=O)=O)=NC%15=C/%11)=C%10C)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HS-291 Related Classifications
HY-157084 Related Classifications
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