ITC Assay Services
Isothermal Titration Calorimetry (ITC) is a sophisticated and powerful analytical technique for measuring the heat changes associated with molecular interactions in solution. By providing direct thermodynamic data, ITC serves as a valuable tool for understanding the binding affinity, stoichiometry, and thermodynamic parameters of molecular interactions. It features high sensitivity and does not require labeling.
How ITC Works
ITC operates by titrating a solution with a ligand (small molecule) into a titrant containing a target macromolecule (such as a protein or nucleic acid) within a calorimeter. As the ligand is added, the binding event occurs, resulting in either the release or absorption of heat. The microcalorimeter constantly measures these heat changes, which are plotted as a titration curve. The area under the peaks in the curve correlates to the amount of heat released or absorbed, allowing for precise calculations of various thermodynamic parameters. Information such as affinity (KD), stoichiometric ratio (n), enthalpy change (ΔH) and entropy change (ΔS) of molecular interactions can be obtained through ITC experiments.
Applications
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Protein-Protein
Interactions -
Protein-Small Molecule
Interactions -
Protein-Nucleic Acid
Interactions -
Nucleic Acid-Small Molecule
Interactions -
Nucleic Acid-Nucleic Acid
Research -
Biomolecule-Cell Research
Key Features
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No fluorescent
labeling or immobilization
required for samples -
High sensitivity and
precision -
No limitations on the
research system -
Thermodynamic
properties can be measured
Quote for Service
ITC Assay Service requires evaluation to determine the appropriate scheme and pricing For additional information about service price and technical specifics, please send an email to [email protected] or contact the sales staff of MedChemExpress directly.