Natrin
Natrin is a cysteine-rich secretory protein (CRISP) toxin found in snake venom that acts as an ion channel modulator. Natrin blocks the large-conductance calcium-activated potassium channel BKCa with an IC50 of 34.4 nM; it acts as a non-competitive antagonist to inhibit the ryanodine binding capacity and calcium release channel activity of RyR1, with an IC50 of 1.03 μM for purified RyR1. Natrin induces vasoconstrictor responses without affecting basal tension. Natrin induces apoptosis in human hepatoma cells, elevating the Bax/Bcl‑2 ratio through the mitochondrial apoptotic pathway and causing metabolic perturbation. Natrin can be used in research on diseases such as hepatocellular carcinoma.
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- CAS No.: 69633-04-1
- Formule: C8H7Cl3O5S
- Masse moléculaire:321.56
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Activité biologique
Description
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Bax |
Bcl-2 |
Kv1.3 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| SMMC-7721 | IC50 |
49.33 mg/L
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Cytotoxicity against human SMMC-7721 hepatocellular carcinoma cells assessed as viability reduction incubated 24 hrs by MTT assay.
Cytotoxicity against human SMMC-7721 hepatocellular carcinoma cells assessed as viability reduction incubated 24 hrs by MTT assay.
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30508545 |
In Vitro
Natrin inhibits the binding of [3H]ryanodine to rabbit skeletal muscle SR vesicles with an IC50 of 3.1 µM and decreases Bmax at 3 µM; it inhibits the binding of [3H]ryanodine to purified RyR1 with an IC50 of 1.03 µM and decreases Bmax at 1 µM[3].
Natrin induces contractile responses in endothelium-denuded mouse thoracic aorta and blocks high-conductance calcium-activated potassium channels, the skeletal muscle isoform of ryanodine receptors, and the voltage-gated potassium channel Kv1.3[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human SMMC-7721 hepatocellular carcinoma cells
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Concentration:25, 50, 75, 100, 150, 200 mg/L
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Incubation Time:24 h
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Result:Inhibited SMMC-7721 proliferation in a concentration-dependent manner.
IC50 = 49.33 mg/L.
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Cell Line:human SMMC-7721 hepatocellular carcinoma cells
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Concentration:25, 50 mg/L
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Incubation Time:24 h
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Result:Induced apoptotic morphological changes in SMMC-7721 cells.
Chemical Information
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CAS No. 69633-04-1
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Masse moléculaire 321.56
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Formule C8H7Cl3O5S
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SMILES
O=S(O)(OCCOC1=CC(Cl)=C(C=C1Cl)Cl)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Wang J, et al. Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel. Biochemistry. 2005 Aug 2;44(30):10145-52. [Content Brief]
[3]. Zhou Q, et al. Structural and functional characterization of ryanodine receptor-natrin toxin interaction. Biophysical journal. 2008 Nov 01;95(9):4289-99. [Content Brief]
[4]. Lu S, et al. Metabolomic study of natrin-induced apoptosis in SMMC-7721 hepatocellular carcinoma cells by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry. International journal of biological macromolecules. 2019 Mar 1;124:1264-73. [Content Brief]
[5]. Averin AS, et al. Cardiovascular Effects of Snake Toxins: Cardiotoxicity and Cardioprotection. Acta naturae. 2021;13(3):4-14. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Natrin Related Classifications
HY-187984 Related Classifications
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