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Cat. No.: HY-N19291

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Perosamine

Name: Perosamine
Brand: MedChemExpress
SKU: HY-N19291
Availability: PreOrder

(Synonyms: D-Perosamine)

Perosamine is a dideoxysugar that can be isolated from the O-antigens of Gram-negative bacteria, including Vibrio cholerae O1 and Escherichia coli O157:H7.

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CAS No.: 31348-80-8
Formule: C₆H₁₃NO₄
Masse moléculaire:163.17

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Perosamine Chemical Structure

CAS No.: 31348-80-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique

Description

Perosamine is a dideoxysugar that can be isolated from the O-antigens of Gram-negative bacteria, including Vibrio cholerae O1 and Escherichia coli O157:H7^[1].

Chemical Information

CAS No. 31348-80-8
Masse moléculaire 163.17
Formule C₆H₁₃NO₄
SMILES

C[C@@H](O)[C@@H](N)[C@H](O)[C@H](O)C=O
Synonyms

D-Perosamine
Initial Source
- Microorganisms
Escherichia coli
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocole

All Histomorphology

Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Cook PD, et al. Accommodation of GDP-linked sugars in the active site of GDP-perosamine synthase. Biochemistry. 2008;47(40):10685-10693. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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