Phaeocaulisin M
Phaeocaulisin M is a guaiane-type sesquiterpene containing a novel 1,2-dioxolane-3-ol structure. Phaeocaulisin M inhibits LPS (HY-D1056)-induced nitric oxide (NO) production in RAW 264.7 macrophages with an IC50 of 6.05 μM, and shows no significant cytotoxicity at the effective concentrations for inhibiting NO production. Phaeocaulisin M is found in the rhizomes of Curcuma phaeocaulis and can be used for research related to inflammatory responses.
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- CAS No.: 1966155-64-5
- Formule: C15H24O5
- Masse moléculaire:284.35
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
6.05 μM
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Inhibition of lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages incubated for 24 h, measured by Griess reagent assay of nitrite concentration in cell supernatant.
Inhibition of lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages incubated for 24 h, measured by Griess reagent assay of nitrite concentration in cell supernatant.
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10286020.2015.1046449 |
In Vitro
Phaeocaulisin M (compound 3) (1 μg/mL LPS; 24 h) inhibits LPS-induced NO production in RAW 264.7 macrophages with an IC50 of 6.05 μM and exhibits no significant cytotoxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1966155-64-5
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Masse moléculaire 284.35
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Formule C15H24O5
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SMILES
OC12C(C(C)(OO2)C)=C[C@@]3([H])[C@](CC[C@@]3(O)C)([H])[C@@](O)(C1)C
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Phaeocaulisin M Related Classifications
HY-N21615 Related Classifications
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