TMA-DPH (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of working solution
Dilute with PBS or serum-free medium (pH 7.4) (optimized according to the experiment). The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining (suspended cells)
2.1 Collect cells by centrifugation and wash twice with PBS for 5 minutes each time.
2.2 TMA-DPH (0.5-5 μM) in Hanks and 20 mM Hepes buffer pH 7.4, and incubate it at 37°C, for 5-30 minutes (optimized according to the experiment).
2.3 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
2.4 Add PBS to wash the cells twice, 5 minutes each time.
2.5 Wash the cells and resuspended in the appropriate Hepes buffer pH 7.4.
2.6 Observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Culture the adherent cells on a sterile coverslip.
3.2 Remove the coverslip from the culture medium and remove the excess culture medium.
3.3 Add TMA-DPH (0.5-5 μM) in Hanks and 20 mM Hepes buffer pH 7.4, and incubate it at 37°C, for 5-30 minutes (optimized according to the experiment).
3.4 Wash the cells and resuspended in the appropriate Hepes buffer pH 7.4.
3.5 Fluorescence microscopy detection (Ex/Em = 355/430 nm).
Note: If flow cytometry is required, the cells need to be digested with trypsin and resuspended before staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.