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TMA-DPH 

Cat. No.: HY-D0986 Purity: >97.0%
Handling Instructions

TMA-DPH is a hydrophobic fluorescent membrane probe.

For research use only. We do not sell to patients.

TMA-DPH Chemical Structure

TMA-DPH Chemical Structure

CAS No. : 115534-33-3

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1 mg USD 96 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

TMA-DPH is a hydrophobic fluorescent membrane probe.

In Vitro

In contact with cells, TMA-DPH is instantaneously incorporated into the plasma membrane. TMA-DPH is concentrated in lysosomes and highly acidic late endosomes[1]. The TMA-DPH fluorescence lifetime remains constant, 6.2±0.2 ns, below the same critical concentration of 2 μM, but decreases significantly with higher concentrations. This decrease, however, slows down above 5 μM. The TMA-DPH fluorescence anisotropy displays a similar evolution to the fluorescence lifetime. It is first constant, 0.283±0.003, again until 2 μM, and then decreases rapidly to 0.270±0.003 with 5 μM, continuing to fall, but at a lower rate with higher concentrations[2].

Molecular Weight

461.62

Formula

C₂₈H₃₁NO₃S

CAS No.

115534-33-3

SMILES

C[N+](C)(C)C1=CC=C(/C=C/C=C/C=C/C2=CC=CC=C2)C=C1.[O-]S(=O)(C3=CC=C(C)C=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

References
Cell Assay
[1]

L929 cells in 2 mL DM10F are allowed to adhere on microscope slide flasks. For the observation of plasma membrane labeling, cells are incubated for a short time (10 s) at room temperature with TMA-DPH 2×10-6 M in PBS or in DM10F from a 4×10-3 M stock solution in dimethylformamide. The unwashed slide is then transferred to the microscope and observed. For the labeling of internalized membrane, the cells are incubated in slide flasks at 37°C, with TMA-DPH 2×10-6 M in DM10F for the desired time, and then washed by gently shaking the slide in PBS for a few seconds[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >97.0%

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TMA-DPH
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