97753-82-7
Chemical Structure
5-Bromo-3-indolyl β-D-galactopyranoside
Synonym(s): Bluo-Gal
- CAS No.: 97753-82-7
- Formula:C14H16BrNO6
- Molecular Weight:374.18
IUPAC Name: (2S,3R,4S,5R,6R)-2-((5-bromo-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
InChIKey: LINMATFDVHBYOS-MBJXGIAVSA-N
SMILES: BrC1=CC=C2C(C(O[C@@H]([C@@H]([C@H]3O)O)O[C@@H]([C@@H]3O)CO)=CN2)=C1
Biological Activity: 5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues[1][2].
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5-Bromo-3-indolyl β-D-galactopyranoside | 99.89% | 5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues. | ||||||||||||||||||||
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- [1]. Gioglio L, et al. An improved method for beta-galactosidase activity detection on muscle tissue. A light and electron microscopic study. Ann Anat. 2002;184(2):153-157. [Content Brief]
- [2]. Blanco MJ, et al. Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos. J Vis Exp. 2018 Jun 26;(136):57785. [Content Brief]
