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Art. -Nr.: HY-100041

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Bromobimane

(Synonyms: Monobromobimane)

8 Cited Publications

Kundenbewertung

Based on 8 publication(s) in Google Scholar

Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols.

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Reinheit: 99.31%
CAS. Nr.: 71418-44-5
Formel: C₁₀H₁₁BrN₂O₂
Molecular Weight:271.11

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Bromobimane Chemical Structure

CAS. Nr.: 71418-44-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols^[1]^[2].

In Vitro

Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
Bromobimane (mBBr) labeled reactive thiols in cells^[1]
(1) Prepare a 100 mM mBBr solution with acetonitrile.
(2) Add 1 mL of 10% cell suspension to 15-25 μL of 100 mM mBBr solution.
(3) After incubation at 37°C for 30-45 min, fluorescence detection was performed.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

450

Excitation (Ex)

344

Chemical Information

CAS. Nr. 71418-44-5
Appearance Solid
Molecular Weight 271.11
Formel C₁₀H₁₁BrN₂O₂
Color Light yellow to yellow
SMILES

O=C1N(C(C(C)=C2C)=O)N2C(CBr)=C1C
Synonyms

Monobromobimane
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (8)

All Cell Imaging/Staining

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Journal Impact Factor

Nat Chem

Genetically programmed cell-based synthesis of non-natural peptide and depsipeptide macrocycles. [Abstract]

2023 Jan;15(1):61-69. PMID: 36550233
J Colloid Interface Sci

Redox-driven hybrid nanoenzyme dynamically activating ferroptosis and disulfidptosis for hepatocellular carcinoma theranostics. [Abstract]

2025 Sep:693:137611. PMID: 40253866

Bromobimane purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Sep:693:137611. [Abstract]

Fluorescence images of the orthotopic liver cancer tumor slices stained with the GSH probe (Bromobimane (blue) collected 24 h after injection of the various agents
Free Radic Biol Med

Sirtuin 7 mitigates renal ferroptosis, fibrosis and injury in hypertensive mice by facilitating the KLF15/Nrf2 signaling. [Abstract]

2022 Nov 3;193(Pt 1):459-473. PMID: 36334846

Bromobimane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2022 Nov 3;193(Pt 1):459-473. [Abstract]

DHE and Bromobimane (MBB: 20 μM) staining revealed the levels of ROS accumulation and GSH content in TCMK-1 cells.
Cell Rep

SNAP25 undergoes phase separation to facilitate the assembly of the synaptic vesicle fusion machinery. [Abstract]

2026 Feb 10;45(2):116983. PMID: 41678336
EMBO Rep

Molecular mechanism of β-arrestin-2 pre-activation by phosphatidylinositol 4,5-bisphosphate. [Abstract]

2024 Oct;25(10):4190-4205. PMID: 39242774
Molecules

The Effects of Different Thiol-Containing Compounds on the Degradation of Sulforaphene. [Abstract]

2024 Sep 12;29(18):4328. PMID: 39339323
Cell Stress Chaperones

Elabela blunts doxorubicin-induced oxidative stress and ferroptosis in rat aortic adventitial fibroblasts by activating the KLF15/GPX4 signaling. [Abstract]

2023 Jan;28(1):91-103. PMID: 36510036

Bromobimane purchased from MedChemExpress. Usage Cited in: Cell Stress Chaperones. 2023 Jan;28(1):91-103. [Abstract]

The condition of intracellular GSH levels assessed by Bromobimane (MBB: 50 μM for 30 min) staining.
Patent

US20250206782A1.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 87.5 mg/mL (322.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (3.69 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6885 mL

18.4427 mL

36.8854 mL

5 mM

0.7377 mL

3.6885 mL

7.3771 mL

10 mM

0.3689 mL

1.8443 mL

3.6885 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kosower EM, et al. Bromobimane probes for thiols. Methods Enzymol. 1995;251:133-48. [Content Brief]

[2]. Newton GL, et al. Determination of biothiols by bromobimane labeling and high-performance liquid chromatography. Methods Enzymol. 1995;251:148-66. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.6885 mL	18.4427 mL	36.8854 mL	92.2135 mL
DMSO	5 mM	0.7377 mL	3.6885 mL	7.3771 mL	18.4427 mL
	10 mM	0.3689 mL	1.8443 mL	3.6885 mL	9.2213 mL
	15 mM	0.2459 mL	1.2295 mL	2.4590 mL	6.1476 mL
	20 mM	0.1844 mL	0.9221 mL	1.8443 mL	4.6107 mL
	25 mM	0.1475 mL	0.7377 mL	1.4754 mL	3.6885 mL
	30 mM	0.1230 mL	0.6148 mL	1.2295 mL	3.0738 mL
	40 mM	0.0922 mL	0.4611 mL	0.9221 mL	2.3053 mL
	50 mM	0.0738 mL	0.3689 mL	0.7377 mL	1.8443 mL
	60 mM	0.0615 mL	0.3074 mL	0.6148 mL	1.5369 mL
	80 mM	0.0461 mL	0.2305 mL	0.4611 mL	1.1527 mL
	100 mM	0.0369 mL	0.1844 mL	0.3689 mL	0.9221 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Bromobimane

8 Cited Publications

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Biologische Aktivität
- Beschreibung
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise

1/16
1/16

Bromobimane purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Sep:693:137611. [Abstract]

Customer Validation
Fluorescence images of the orthotopic liver cancer tumor slices stained with the GSH probe (Bromobimane (blue) collected 24 h after injection of the various agents
1/16

Bromobimane purchased from MedChemExpress. Usage Cited in: Cell Stress Chaperones. 2023 Jan;28(1):91-103. [Abstract]

Customer Validation
The condition of intracellular GSH levels assessed by Bromobimane (MBB: 50 μM for 30 min) staining.
1/16

Bromobimane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2022 Nov 3;193(Pt 1):459-473. [Abstract]

Customer Validation
DHE and Bromobimane (MBB: 20 μM) staining revealed the levels of ROS accumulation and GSH content in TCMK-1 cells.

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Bromobimane

Publications Citing Use of MedChemExpress (MCE) Bromobimane

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bromobimane Related Classifications

HY-100041 Related Classifications