Art. -Nr.: HY-D1027

Dansylcadaverine

(Synonyms: Monodansyl cadaverine)

14 Cited Publications

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Based on 14 publication(s) in Google Scholar

Dansylcadaverine (Monodansyl cadaverine) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.

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Reinheit: 98.80%
CAS. Nr.: 10121-91-2
Formel: C₁₇H₂₅N₃O₂S
Molecular Weight:335.46

Speicherung:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen)

Dansylcadaverine Chemical Structure

CAS. Nr.: 10121-91-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Dyeing Example
Chemical Information
Publications (14)
Lösungsmittel & Löslichkeit
- In Vitro
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

The inhibitory activity of dansylcadaverine reflects its ability to serve as a substrate for transglutaminases and to block competitively the crosslinking of fibrin molecules^[2].
Dansylcadaverine, a cationic fluorescent probe binds to bacterial lipopolysaccharide and lipid A, and is displaced competitively by other compounds which possess affinity toward endotoxins^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dyeing Example

Chemical Information

CAS. Nr. 10121-91-2
Appearance Solid
Molecular Weight 335.46
Formel C₁₇H₂₅N₃O₂S
Color White to off-white
SMILES

NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O
Synonyms

Monodansyl cadaverine
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen)

Publications (14)

All IFBio/Physico-chemical AssayFlow CytometryHistological Imaging/StainingWB

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Journal Impact Factor

Gastroenterology

Clusterin Drives Fiber Endocytosis by Mesothelial Cells to Resolve Liver Fibrosis. [Abstract]

2025 Dec 10:S0016-5085(25)05907-4. PMID: 41369634

Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]

Endocytosis inhibitor Dansylcadaverine (DC) (80 μM; i.v.; twice a week) inhibited the endocytosis of OG-collagen IV (green) by mesothelial cells.

Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]

Dansylcadaverine (DC) (80 μM; i.v.; twice a week) significantly inhibited the endocytosis effect of collagen by mesothelial cells mediated by CLUtide-3-LRP2 interaction.

Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]

SR and Masson staining in fibrotic livers from Clu^-/- mice treated with Dansylcadaverine (DC) (80 μM; i.v.; twice a week) as indicated.

Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]

Col1A1 and quantification in fibrotic livers from Clu^-/- mice treated with Dansylcadaverine (DC) (80 μM; i.v.; twice a week) as indicated.
J Extracell Vesicles

Syntenin Controls Extracellular Vesicle-Induced Tumour Migration by Regulating the Expression of Adhesion Proteins on Small Extracellular Vesicles. [Abstract]

2025 Aug;14(8):e70133. PMID: 40831280

Dansylcadaverine purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70133. [Abstract]

Dansylcadaverine (Da) (180 µM; 24 h) was able to efficiently block vesicle incorporation into 4T1 cells by 50% at a concentration that was non-toxic for the cells.
Microbiome

A tale for two roles: Root-secreted methyl ferulate inhibits P. nicotianae and enriches the rhizosphere Bacillus against black shank disease in tobacco. [Abstract]

2025 Jan 31;13(1):33. PMID: 39891202
Int J Biol Sci

MicroRNA-216b targets HK2 to potentiate autophagy and apoptosis of breast cancer cells via the mTOR signaling pathway. [Abstract]

2021 Jul 13;17(11):2970-2983. PMID: 34345220
New Phytol

Lipid metabolism and autophagy maintain cellular homeostasis under chloroplast dysfunction in Chlamydomonas reinhardtii. [Abstract]

2026 Jun;250(5):3084-3102. PMID: 41999165
Br J Pharmacol

A mitochondria-targeting and G-quadruplex structure-binding ligand inducing calcium overload and ferroptosis in human cancer cells. [Abstract]

2025 Aug;182(16):3923-3951. PMID: 40344208
Biomed Pharmacother

Pseudolaric acid B suppresses NSCLC progression through the ROS/AMPK/mTOR/autophagy signalling pathway. [Abstract]

2024 Jun:175:116614. PMID: 38670047
Cell Prolif

Deletion of Tgm2 suppresses BMP-mediated hepatocyte-to-cholangiocyte metaplasia in ductular reaction. [Abstract]

2024 Apr 16:e13646. PMID: 38623945
Int J Pharm

ClC-3 silencing mediates lysosomal acidification arrest and autophagy inhibition to sensitize chemo-photothermal therapy. [Abstract]

2022 Nov 25:628:122297. PMID: 36261097
Neurochem Res

Kaemperfol Protects Dopaminergic Neurons by Promoting mTOR-Mediated Autophagy in Parkinson's Disease Models. [Abstract]

2023 May;48(5):1395-1411. PMID: 36469163
Biochim Biophys Acta Mol Cell Res

TGM2 positively regulates myoblast differentiation via enhancing the mTOR signaling. [Abstract]

2022 Mar;1869(3):119173. PMID: 34902478
Toxicol Appl Pharmacol

Targeting autophagy by natural product Ursolic acid for prevention and treatment of osteoporosis. [Abstract]

2020 Dec 15;409:115271. PMID: 33065153
bioRxiv

Insulin-Like Growth Factor 1 Receptor Regulates Breast Cancer Cell Adhesion through Beta-1 Integrin. [Abstract]

2025 Sep 16:2025.09.11.674989. PMID: 41000977
Res Sq

2024 Aug 07.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 62.5 mg/mL (186.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9810 mL

14.9049 mL

29.8098 mL

5 mM

0.5962 mL

2.9810 mL

5.9620 mL

10 mM

0.2981 mL

1.4905 mL

2.9810 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Protokoll

Kinase Assay^[1]

To determine the time course of transglutamination, thymosin β₄ (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl₂, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay

MCF7 cells (2.4×10⁴) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. [Content Brief]

[2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9. [Content Brief]

[3]. Gao L, et al. Autophagy blockade sensitizes human head and neck squamous cell carcinoma towards CYT997 through enhancing excessively high reactive oxygen species-induced apoptosis. J Mol Med (Berl). 2018;96(9):929-938. [Content Brief]

[4]. Cornwell MM, et al. Inhibition of the adhesion of Chinese hamster ovary cells by the naphthylsulfonamides dansylcadaverine and N-(6-aminohexyl)-5-chloro-1-naphthylenesulfonamide (W7). Biochim Biophys Acta. 1983;762(3):414-419. [Content Brief]

[5]. David SA, et al. Analysis of the binding of polymyxin B to endotoxic lipid A and core glycolipid using a fluorescent displacement probe. Biochim Biophys Acta. 1992;1165(2):147-152. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9810 mL	14.9049 mL	29.8098 mL	74.5245 mL
	5 mM	0.5962 mL	2.9810 mL	5.9620 mL	14.9049 mL
	10 mM	0.2981 mL	1.4905 mL	2.9810 mL	7.4525 mL
	15 mM	0.1987 mL	0.9937 mL	1.9873 mL	4.9683 mL
	20 mM	0.1490 mL	0.7452 mL	1.4905 mL	3.7262 mL
	25 mM	0.1192 mL	0.5962 mL	1.1924 mL	2.9810 mL
	30 mM	0.0994 mL	0.4968 mL	0.9937 mL	2.4842 mL
	40 mM	0.0745 mL	0.3726 mL	0.7452 mL	1.8631 mL
	50 mM	0.0596 mL	0.2981 mL	0.5962 mL	1.4905 mL
	60 mM	0.0497 mL	0.2484 mL	0.4968 mL	1.2421 mL
	80 mM	0.0373 mL	0.1863 mL	0.3726 mL	0.9316 mL
	100 mM	0.0298 mL	0.1490 mL	0.2981 mL	0.7452 mL

Keywords

Dansylcadaverine

14 Cited Publications

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Dansylcadaverine

Publications Citing Use of MedChemExpress (MCE) Dansylcadaverine

Biologische Aktivität

Chemical Information

Publications (14)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Dansylcadaverine Related Classifications

HY-D1027 Related Classifications