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Art. -Nr.: HY-W612175

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H-Ala-pNA

H-Ala-pNA is an L-amino acid p-nitroaniline (pNA) derivative and a specific substrate for leukotriene A4 hydrolase. The D-enantiomer of H-Ala-pNA shows no activity toward leukotriene A4 hydrolase. H-Ala-pNA can be catalytically hydrolyzed by leukotriene A4 hydrolase, and the p-nitroaniline produced during the reaction is monitored spectrophotometrically at 405 nm to enable quantitative detection of enzyme activity. H-Ala-pNA is used to evaluate the potency of inhibitors targeting the amidase activity of leukotriene A4 hydrolase.

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CAS. Nr.: 1668-13-9
Formel: C₉H₁₁N₃O₃
Molecular Weight:209.20

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

H-Ala-pNA Chemical Structure

CAS. Nr.: 1668-13-9

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Leukotriene Receptor Isoform-spezifische Produkte anzeigen :

In Vitro

H-Ala-pNA (1.5 mM) is hydrolyzed by purified human leukocyte leukotriene A₄ hydrolase with a K_m of 500 μM, kcat of 6.0×10^-1 s^-1, Vmax of 530 nmol/min per mg, and kcat/K_m of 1.2×10³ M^-1s^-1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

Chemical Information

CAS. Nr. 1668-13-9
Molecular Weight 209.20
Formel C₉H₁₁N₃O₃
SMILES

C[C@H](N)C(NC1=CC=C(C=C1)N(=O)=O)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Yuan W, et al. Development of selective tight-binding inhibitors of leukotriene A4 hydrolase. J Med Chem. 1993;36(2):211-220. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

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100 mg

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