MOPS
Based on 1 publication(s) in Google Scholar
MOPS is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. MOPS significantly improves the thermal stability of BSA (HY-D0842).
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- Reinheit: 99.95%
- CAS. Nr.: 1132-61-2
- Formel: C7H15NO4S
- Molecular Weight:209.26
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Speicherung:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MOPS
More
Biologische Aktivität
Preparation of Keratinocyte and Microbial Culture Medium Using MOPS[3] Materials:
MOPS: 164 mM
RPMI-1640 medium
L-glutamine: 2 mM
Sodium bicarbonate: 2.0 g/L
Sterile phosphate-buffered saline (PBS, pH 7.2)
Normal oral keratinocytes (NOK) or human keratinocyte cell line (HaCat)
Microorganisms: Candida albicans SC5314, Staphylococcus aureus ATCC25923
Sterile water
Steps:
1. Preparation of MOPS Buffer:
Dissolve 164 mM of MOPS in 1 L of sterile water, and adjust the pH to 7.0.
Add 2 mM of L-glutamine and 2.0 g/L of sodium bicarbonate, stirring until fully dissolved.
Filter the solution using a sterile filtration device to ensure sterility.
2. Preparation of Keratinocyte Culture Medium:
Mix the prepared MOPS buffer with RPMI-1640 medium at a 1:1 ratio to obtain MOPS-containing medium.
Seed NOK or HaCat cells into a 24-well plate, adding approximately 4.5 × 104 cells per well with an appropriate amount of RPMI/MOPS medium.
Incubate the cells in a 37°C, 5% CO2 incubator until confluent (approximately 48 hours).
3. Preparation of Microbial Culture Medium:
Prepare the Candida albicans and Staphylococcus aureus strains, culturing them in suitable media until they reach the logarithmic growth phase.
Resuspend the microorganisms in MOPS medium at a concentration of approximately 107 cells/mL.
Inoculate the microorganisms into a 24-well plate, adding an appropriate amount of MOPS medium, and incubate at 37°C in a shaker at 75 rpm for 90 minutes.
4. Co-culture of Keratinocytes and Microorganisms:
Co-culture keratinocytes and microorganisms in a 24-well plate, adding MOPS medium to maintain the pH around 7.0.
The co-culture duration can be set based on experimental needs, with a recommendation to limit the time to under 12 hours to reduce the cytotoxicity of MOPS.
5. Cell Viability Assay:
Use the MTT assay to measure cell viability, sampling every 2 hours, adding MTT/PBS solution, incubating at 37°C for 4 hours, and recording the results.
Notes:
MOPS is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 1132-61-2
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Appearance Solid
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Molecular Weight 209.26
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Formel C7H15NO4S
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Color White to off-white
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SMILES
O=S(CCCN1CCOCC1)(O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Publications (1)
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Journal Impact Factor
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Most Recent
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Curr Microbiol
Berberine Hydrochloride Enhances the Fluconazole Susceptibility of Candida albicans through Multiple Targets. [Abstract]2026 Mar 27;83(5):262. PMID: 41896451
Lösungsmittel & Löslichkeit
H2O : 100 mg/mL (477.87 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: ≥ 100 mg/mL (477.87 mM); Clear solution
Reinheit & Dokumentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Steven D Carson, et al. MOPS and coxsackievirus B3 stability. Virology. 2017 Jan 15;501:183-187. [Content Brief]
[2]. Juliane Schmidt, et al. Effect of Tris, MOPS, and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases. FEBS Open Bio. 2016 Jul 20;6(9):919-27. [Content Brief]
[3]. Gupta BS, et al. Buffers more than buffering agent: introducing a new class of stabilizers for the protein BSA. Phys Chem Chem Phys. 2015 Jan 14;17(2):1114-33. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.7787 mL | 23.8937 mL | 47.7874 mL | 119.4686 mL |
| 5 mM | 0.9557 mL | 4.7787 mL | 9.5575 mL | 23.8937 mL | |
| 10 mM | 0.4779 mL | 2.3894 mL | 4.7787 mL | 11.9469 mL | |
| 15 mM | 0.3186 mL | 1.5929 mL | 3.1858 mL | 7.9646 mL | |
| 20 mM | 0.2389 mL | 1.1947 mL | 2.3894 mL | 5.9734 mL | |
| 25 mM | 0.1911 mL | 0.9557 mL | 1.9115 mL | 4.7787 mL | |
| 30 mM | 0.1593 mL | 0.7965 mL | 1.5929 mL | 3.9823 mL | |
| 40 mM | 0.1195 mL | 0.5973 mL | 1.1947 mL | 2.9867 mL | |
| 50 mM | 0.0956 mL | 0.4779 mL | 0.9557 mL | 2.3894 mL | |
| 60 mM | 0.0796 mL | 0.3982 mL | 0.7965 mL | 1.9911 mL | |
| 80 mM | 0.0597 mL | 0.2987 mL | 0.5973 mL | 1.4934 mL | |
| 100 mM | 0.0478 mL | 0.2389 mL | 0.4779 mL | 1.1947 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
MOPS Related Classifications
HY-D0859 Related Classifications
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