Art. -Nr.: HY-105129A

Pimonidazole

9 Cited Publications

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Based on 9 publication(s) in Google Scholar

Pimonidazole is a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in tumor. Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after reduction of its nitro group, it can be used for qualitative and quantitative assessment of tumor hypoxia .

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Reinheit: 99.32%
CAS. Nr.: 70132-50-2
Formel: C₁₁H₁₈N₄O₃
Molecular Weight:254.29

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Pimonidazole Chemical Structure

CAS. Nr.: 70132-50-2

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Publications (9)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Pimonidazole is a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in tumor^[1]. Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after reduction of its nitro group, it can be used for qualitative and quantitative assessment of tumor hypoxia ^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-AA8	IC₅₀	3.03 mM Compound: 2	Aerobic growth inhibition in Chinese hamster cell line AA8 Aerobic growth inhibition in Chinese hamster cell line AA8	[PMID: 7783125]

In Vitro

Pimonidazole, the exogenous hypoxia marker, is a 2-nitroimidazole compound, which forms covalent bonds with cellular macromolecules at oxygen levels below 1.3%.
Detection: Hypoxic cells were recognized by immunohistochemical detection of pimonidazole using a mouse monoclonal antibody. Cell proliferation was detected with a commercially available monoclonal antibody for proliferating cell nuclear antigen (PCNA). Assessment of hypoxia and cell proliferation was made qualitatively with light microscopy and quantitatively using point counting and image analysis software methods.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 70132-50-2
Appearance Solid
Molecular Weight 254.29
Formel C₁₁H₁₈N₄O₃
Color Light yellow to yellow
SMILES

OC(CN1C=CN=C1[N+]([O-])=O)CN2CCCCC2
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (9)

All Flow CytometryHistological Imaging/Staining

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Journal Impact Factor

Theranostics

Neuronal STAT3/HIF-1α/PTRF axis-mediated bioenergetic disturbance exacerbates cerebral ischemia-reperfusion injury via PLA2G4A. [Abstract]

2022 Apr 4;12(7):3196-3216. PMID: 35547748
Asian J Pharm Sci

Tumor microenvironment-responsive artesunate loaded Z-scheme heterostructures for synergistic photo-chemodynamic therapy of hypoxic tumor. [Abstract]

2023 May;18(3):100798. PMID: 37252037
Mater Today Bio

"Top-down and Bottom-up" cholesterol-depleting biomimetic nanoparticle for enhancing sonodynamic therapy against hepatocellular carcinoma. [Abstract]

2025 Sep 30:35:102370. PMID: 41104047
J Colloid Interface Sci

Self-amplifying hypoxia cascade in covalent organic framework/metal organic framework nanoreactors for synergistic cancer therapy. [Abstract]

2025 Dec 3:706:139602. PMID: 41353990

Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Dec 3:706:139602. [Abstract]

The hypoxia level in 4T1 cells was quantitatively assessed using Pimonidazole hydrochloride (PIMO) (100 μM; 60 min) as a probe.

Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Dec 3:706:139602. [Abstract]

Representative fluorescence images of tumor slices from 4 T1 tumor-bearing mice after various treatments were stained for hypoxia with Pimonidazole hydrochloride (PIMO) (60 mg/kg; i.p.; 90 min).
J Colloid Interface Sci

A multifunctional oxidative stress amplifier for synergistic disruption of redox homeostasis and enhanced cancer therapy. [Abstract]

2025 Aug 28;702(Pt 1):138870. PMID: 40902490

Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Aug 28;702(Pt 1):138870. [Abstract]

Representative fluorescence images of tumor slices from mice bearing 4 T1 tumors stained with Pimonidazole hydrochloride (PIMO) (60 mg/kg; i.p.; 90 min).
J Colloid Interface Sci

Covalent organic frameworks-derived carbon nanospheres based nanoplatform for tumor specific synergistic therapy via oxidative stress amplification and calcium overload. [Abstract]

2024 May:661:908-922. PMID: 38330663

Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2024 May:661:908-922. [Abstract]

Fluorescence images of tumor slices from mice bearing 4T1 tumors stained with the hypoxia probe Pimonidazole hydrochloride (PIMO) (60 mg/kg; i.p.; 90 min) after different treatments were shown.
Sci Rep

Contribution of HIF-1α/BNIP3-mediated autophagy to lipid accumulation during irinotecan-induced liver injury. [Abstract]

2023 Apr 21;13(1):6528. PMID: 37085612
Exp Neurol

By inhibiting pyroptosis to reduce neuroinflammation, PEG-bHb may prevent the development of secondary injury after traumatic brain injury. [Abstract]

2025 Aug 27:115447. PMID: 40882799
Exp Eye Res

Zeaxanthin improves myopia by regulating the HIF-1α-glycolysis signaling pathway. [Abstract]

2025 Oct:259:110557. PMID: 40738388

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (196.63 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (3.93 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9325 mL

19.6626 mL

39.3252 mL

5 mM

0.7865 mL

3.9325 mL

7.8650 mL

10 mM

0.3933 mL

1.9663 mL

3.9325 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Varia MA, et al. Pimonidazole: a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in cervical carcinoma. Gynecol Oncol. 1998 Nov;71(2):270-7. [Content Brief]

[2]. Masaki Y, et al. Imaging Mass Spectrometry Revealed the Accumulation Characteristics of the 2-Nitroimidazole-Based Agent "Pimonidazole" in Hypoxia. PLoS One. 2016 Aug 31;11(8):e0161639. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.9325 mL	19.6626 mL	39.3252 mL	98.3130 mL
DMSO	5 mM	0.7865 mL	3.9325 mL	7.8650 mL	19.6626 mL
	10 mM	0.3933 mL	1.9663 mL	3.9325 mL	9.8313 mL
	15 mM	0.2622 mL	1.3108 mL	2.6217 mL	6.5542 mL
	20 mM	0.1966 mL	0.9831 mL	1.9663 mL	4.9156 mL
	25 mM	0.1573 mL	0.7865 mL	1.5730 mL	3.9325 mL
	30 mM	0.1311 mL	0.6554 mL	1.3108 mL	3.2771 mL
	40 mM	0.0983 mL	0.4916 mL	0.9831 mL	2.4578 mL
	50 mM	0.0787 mL	0.3933 mL	0.7865 mL	1.9663 mL
	60 mM	0.0655 mL	0.3277 mL	0.6554 mL	1.6385 mL
	80 mM	0.0492 mL	0.2458 mL	0.4916 mL	1.2289 mL
	100 mM	0.0393 mL	0.1966 mL	0.3933 mL	0.9831 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Pimonidazole

9 Cited Publications

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Pimonidazole

Publications Citing Use of MedChemExpress (MCE) Pimonidazole

Biologische Aktivität

Chemical Information

Publications (9)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Pimonidazole Related Classifications

HY-105129A Related Classifications