Art. -Nr.: HY-150279

PolQi2

3 Cited Publications

Kundenbewertung

Based on 3 publication(s) in Google Scholar

PolQi2 is a PolΘ inhibitor that targets and inhibits alt-EJ (alternative end-joining) repair by inhibiting the helicase domain at the N-terminus of PolΘ. PolQi2 enhances the precision and integration efficiency of gene editing at different loci and in various cell lines. Furthermore, the combined use of PolQi2 with DNA-PK inhibitors reduces the off-target effects of Cas9. PolQi2 can be used in gene editing research.

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Reinheit: 98.91%
CAS. Nr.: 2565638-16-4
Formel: C₂₁H₁₆ClN₅O₃S
Molecular Weight:453.90

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

PolQi2 Chemical Structure

CAS. Nr.: 2565638-16-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (3)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Helicase

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

In Vitro

PolQi2 (3 μM, 14 days), in combination with DNA-PK inhibitors (1 μM, 14 days), efficiently and stably achieves targeted gene insertion in HeLa and Jurkat cells, without adversely affecting cell proliferation [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 2565638-16-4
Appearance Solid
Molecular Weight 453.90
Formel C₂₁H₁₆ClN₅O₃S
Color White to off-white
SMILES

O=C(C1=CC=NC=C1C2=C(OC)C=CC=C2)NC3=NN=C(S3)OCC4=NC=C(C=C4)Cl
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Journal Impact Factor

Nat Biotechnol

Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations. [Abstract]

2024 Nov 27. PMID: 39604565

PolQi2 purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2024 Nov 27. [Abstract]

Quantification by long-read sequencing of the fraction of total reads with a length greater than a defined size and the quantified frequency of deletions larger than 1 kb in edited RPE-1 p53^−/− cells was performed. Editing with combined AZD7648 and PolQi2 (3 µM; 3 d) reduced AZD7648-induced kilobase-scale deletions.

PolQi2 purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2024 Nov 27. [Abstract]

Editing with combined AZD7648 and PolQi2 (3 μM; 3 d) maintained elevated HDR levels. Frequencies of HDR and indels detected by short-read sequencing in RPE-1 p53^−/− and RPE-1 p53^+/+ cells edited at HBB, CCR5, and GAPDH were recorded.
bioRxiv

2025 Nov 29.

PolQi2 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Nov 29.

PolQi2, a potent Polθ inhibitor, suppresses the MMEJ repair pathway and enhances HDR efficiency during CRISPR/Cas9-mediated gene editing. Splenic T cells from transgenic Cas9 mice were activated with Dynabeads and transduced with the specified MOI of AAV after 48 hours in 1 μM AZD7468 and 3 μM PolQi2. Percentage of cells expressing GFP above baseline level was determined by flow cytometry 5 days after transduction.

PolQi2 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Nov 29.

Knock-in efficiency was assessed by flow cytometry as the percentage of GFP+ cells among CD3- cells. Treatment with AZD7648 and PolQi2 (3 μM) resulted in the highest editing efficiency, achieving a knock-in rate of up to 80% under AAV transduction conditions.
bioRxiv

Structural Basis for Retron Co-option of Anti-phage ATPase-nuclease. [Abstract]

2025 May 13:2025.05.10.653283. PMID: 40462907

PolQi2 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 May 13:2025.05.10.653283. [Abstract]

Quantification of Ec78 retron precise editing was performed in comparison with no reverse transcriptase control (No RT), in the absence (Ec78) or the presence (Ec78 + inhib) of inhibitors, AZD7648 and PolQi2 (3 μM; 48 h), and the same experiments were conducted with the Mestre-1531 retron (RT 1531) using the same inhibitors.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 70 mg/mL (154.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2031 mL

11.0156 mL

22.0313 mL

5 mM

0.4406 mL

2.2031 mL

4.4063 mL

10 mM

0.2203 mL

1.1016 mL

2.2031 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (5.51 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (5.51 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. imberger S, et al. Simultaneous inhibition of DNA-PK and Polθ improves integration efficiency and precision of genome editing. Nat Commun. 2023 Aug 14;14(1):4761. [Content Brief]

[2]. Antoniou P, et al. Improved nuclease-based prime editing by DNA repair modulation and pegRNA engineering[J]. BioRxiv, 2024: 2024.02. 01.578377.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2031 mL	11.0156 mL	22.0313 mL	55.0782 mL
	5 mM	0.4406 mL	2.2031 mL	4.4063 mL	11.0156 mL
	10 mM	0.2203 mL	1.1016 mL	2.2031 mL	5.5078 mL
	15 mM	0.1469 mL	0.7344 mL	1.4688 mL	3.6719 mL
	20 mM	0.1102 mL	0.5508 mL	1.1016 mL	2.7539 mL
	25 mM	0.0881 mL	0.4406 mL	0.8813 mL	2.2031 mL
	30 mM	0.0734 mL	0.3672 mL	0.7344 mL	1.8359 mL
	40 mM	0.0551 mL	0.2754 mL	0.5508 mL	1.3770 mL
	50 mM	0.0441 mL	0.2203 mL	0.4406 mL	1.1016 mL
	60 mM	0.0367 mL	0.1836 mL	0.3672 mL	0.9180 mL
	80 mM	0.0275 mL	0.1377 mL	0.2754 mL	0.6885 mL
	100 mM	0.0220 mL	0.1102 mL	0.2203 mL	0.5508 mL

Keywords

PolQi2

3 Cited Publications

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PolQi2

Cell Cycle/DNA Damage Compound Library

Anti-Aging Compound Library

Anti-Cardiovascular Disease Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Publications Citing Use of MedChemExpress (MCE) PolQi2

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

PolQi2

Cell Cycle/DNA Damage Compound Library

Anti-Aging Compound Library

Anti-Cardiovascular Disease Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Publications Citing Use of MedChemExpress (MCE) PolQi2

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

PolQi2 Related Classifications

HY-150279 Related Classifications