4-Diethylaminobenzaldehyde
Based on 7 publication(s) in Google Scholar
4-Diethylaminobenzaldehyde is a reversible aldehyde dehydrogenases (ALDHs) inhibitor, with a Ki of 4 nM for ALDH1. 4-Diethylaminobenzaldehyde displays potent anti-androgenic effect (IC50= 1.71μM).
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- Pureté : 99.98%
- CAS No.: 120-21-8
- Formule: C11H15NO
- Masse moléculaire:177.24
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Stockage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 4-Diethylaminobenzaldehyde
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Flow Cytometry
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Flow Cytometry
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RT-PCR
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| DU-145 | IC50 |
>200 μM
Compound: DEAB
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Antiproliferative activity against human DU145 cells expressing ALDH1A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay
Antiproliferative activity against human DU145 cells expressing ALDH1A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay
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[PMID: 35212533] |
| LNCaP | IC50 |
>200 μM
Compound: DEAB
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Antiproliferative activity against human LNCaP cells expressing ALDH1A3 assessed as reduction in cell survival measured after 96 hrs by MTT assay
Antiproliferative activity against human LNCaP cells expressing ALDH1A3 assessed as reduction in cell survival measured after 96 hrs by MTT assay
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[PMID: 35212533] |
| PC-3 | IC50 |
>200 μM
Compound: DEAB
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Antiproliferative activity against human PC-3 cells expressing ALDH1A1 and ALDH3A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay
Antiproliferative activity against human PC-3 cells expressing ALDH1A1 and ALDH3A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay
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[PMID: 35212533] |
In Vitro
4-Diethylaminobenzaldehyde (DEAB) is reversible competitive inhibitor of ALDH1 (competitive with the aldehyde substrate) with a Ki value of 4 nM, and irreversibly inactivates ALDH7A1 via formation of a stable, covalent acyl-enzyme species[1].
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The Temozolomide (TMZ)-resistant cell line LN18 is treated with a combination of TMZ and 4-Diethylaminobenzaldehyde for 10 days. As indicated by colony formation assay, the clonogenic capacity of LN18 cells exposed to TMZ in combination with 4-Diethylaminobenzaldehyde (DEAB) is significantly attenuated[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 120-21-8
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Appearance Solid
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Masse moléculaire 177.24
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Formule C11H15NO
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Color Yellow to brown
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SMILES
O=CC1=CC=C(N(CC)CC)C=C1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (7)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Retinol Binding Protein 4 reactivates latent HIV-1 by triggering canonical NF-κB, JAK/STAT5 and JNK signalling. [Abstract]2025 Oct 3;10(1):326. PMID: 41038866
4-Diethylaminobenzaldehyde purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Oct 3;10(1):326. [Abstract]
4-Diethylaminobenzaldehyde (DEAB; 800 μM; 5+48 h) inhibited the conversion of retinal to retinoic acid upon delivery by holo-RBP4 in J-Lat 10.6 cells.
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Mol Cancer
Combined targeting of GPX4 and BCR-ABL tyrosine kinase selectively compromises BCR-ABL+ leukemia stem cells. [Abstract]2024 Oct 28;23(1):240. PMID: 39465372 -
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Nat Commun
Notch and retinoic acid signals regulate macrophage formation from endocardium downstream of Nkx2-5. [Abstract]2023 Sep 5;14(1):5398. PMID: 37669937
4-Diethylaminobenzaldehyde purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Sep 5;14(1):5398. [Abstract]
4-Diethylaminobenzaldehyde (DEAB; 0, 10, 50, 100, 250 μM; 4 days) decreased the retinoic acid receptor-β (RARβ) mRNA expression level in the mouse embryo.
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4-Diethylaminobenzaldehyde purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 March 02.
4-Diethylaminobenzaldehyde (DEAB; 200, 400, 800 μM; 4+48 h) inhibiting conversion of retinal to retinoic acid and subsequently left untreated or treated with 611 TNFα or the indicated concentrations of RBP4 in J-Lat cells.
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (564.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.25 mg/mL (18.34 mM); Clear solution
This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Luo M, et al. Diethylaminobenzaldehyde is a covalent, irreversible inactivator of ALDH7A1. ACS Chem Biol. 2015 Mar 20;10(3):693-7. [Content Brief]
[2]. Araki N, et al. Screening for androgen receptor activities in 253 industrial chemicals by in vitro reporter gene assays using AR-EcoScreen cells. Toxicol In Vitro. 2005 Sep;19(6):831-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.6421 mL | 28.2103 mL | 56.4207 mL | 141.0517 mL |
| 5 mM | 1.1284 mL | 5.6421 mL | 11.2841 mL | 28.2103 mL | |
| 10 mM | 0.5642 mL | 2.8210 mL | 5.6421 mL | 14.1052 mL | |
| 15 mM | 0.3761 mL | 1.8807 mL | 3.7614 mL | 9.4034 mL | |
| 20 mM | 0.2821 mL | 1.4105 mL | 2.8210 mL | 7.0526 mL | |
| 25 mM | 0.2257 mL | 1.1284 mL | 2.2568 mL | 5.6421 mL | |
| 30 mM | 0.1881 mL | 0.9403 mL | 1.8807 mL | 4.7017 mL | |
| 40 mM | 0.1411 mL | 0.7053 mL | 1.4105 mL | 3.5263 mL | |
| 50 mM | 0.1128 mL | 0.5642 mL | 1.1284 mL | 2.8210 mL | |
| 60 mM | 0.0940 mL | 0.4702 mL | 0.9403 mL | 2.3509 mL | |
| 80 mM | 0.0705 mL | 0.3526 mL | 0.7053 mL | 1.7631 mL | |
| 100 mM | 0.0564 mL | 0.2821 mL | 0.5642 mL | 1.4105 mL |
4-Diethylaminobenzaldehyde Related Classifications
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