Ac4GlcNAz
Based on 1 Customer Validation
Ac4GlcNAz (N-azidoacetylglucosamine-tetraacylated) is an azido-containing fully acetylated N-acetylglucosamine analog, a metabolic glycan marker, and also an inducer of S-glycosylation. Ac4GlcNAz can be taken up by bacteria, processed via endogenous carbohydrate biosynthesis pathways, and integrated into glycans by replacing natural monosaccharides. Under alkali promotion, Ac4GlcNAz undergoes β-elimination to form α,β-unsaturated aldehydes, which then bind to cysteine residues via Michael addition. Ac4GlcNAz can be integrated into glycoproteins. Ac4GlcNAz is applicable to research related to Helicobacter pylori infection.
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- Pureté: 99.49%
- CAS No.: 98924-81-3
- Formule: C16H22N4O10
- Masse moléculaire:430.37
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Stockage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Ac4GlcNAz (1 mM; 3 days) is robustly incorporated into glycans in Plesiomonas shigelloides ATCC 51903 bacterial cells[1].
Ac4GlcNAz (1 mM; 2 h) induces robust S-glyco-modification (non-specific cysteine labeling) in HeLa cell lysates at 37°C[3].
Ac4GlcNAz (200 μM; 24 h) efficiently labels proteins in living HeLa cells[3].
Ac4GlcNAz (200 μM; 48 h) is non-cytotoxic to HeLa cells and 293T cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:200 μM
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Incubation Time:24 h
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Result:Efficiently labeled proteins in living HeLa cells, producing strong signals on western blot.
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Cell Line:HeLa cells, 293T cells
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Concentration:200 μM
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Incubation Time:48 h
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Result:Showed no obvious cytotoxicity to HeLa cells.
Showed no obvious cytotoxicity to 293T cells.
Chemical Information
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CAS No. 98924-81-3
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Appearance Solid
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Masse moléculaire 430.37
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Formule C16H22N4O10
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Color White to off-white
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SMILES
CC(O[C@@H]1[C@H](C(O[C@@H]([C@H]1OC(C)=O)COC(C)=O)OC(C)=O)NC(CN=[N+]=[N-])=O)=O
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Synonyms
N-azidoacetylglucosamine-tetraacylated
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (232.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Luong P, et al. Synthesis and Application of Rare Deoxy Amino l-Sugar Analogues to Probe Glycans in Pathogenic Bacteria. ACS infectious diseases. 2022 Apr 08;8(4):889-900. [Content Brief]
[2]. Clark EL, et al. Development of Rare Bacterial Monosaccharide Analogs for Metabolic Glycan Labeling in Pathogenic Bacteria. ACS chemical biology. 2016 Dec 16;11(12):3365-3373. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3236 mL | 11.6179 mL | 23.2358 mL | 58.0896 mL |
| 5 mM | 0.4647 mL | 2.3236 mL | 4.6472 mL | 11.6179 mL | |
| 10 mM | 0.2324 mL | 1.1618 mL | 2.3236 mL | 5.8090 mL | |
| 15 mM | 0.1549 mL | 0.7745 mL | 1.5491 mL | 3.8726 mL | |
| 20 mM | 0.1162 mL | 0.5809 mL | 1.1618 mL | 2.9045 mL | |
| 25 mM | 0.0929 mL | 0.4647 mL | 0.9294 mL | 2.3236 mL | |
| 30 mM | 0.0775 mL | 0.3873 mL | 0.7745 mL | 1.9363 mL | |
| 40 mM | 0.0581 mL | 0.2904 mL | 0.5809 mL | 1.4522 mL | |
| 50 mM | 0.0465 mL | 0.2324 mL | 0.4647 mL | 1.1618 mL | |
| 60 mM | 0.0387 mL | 0.1936 mL | 0.3873 mL | 0.9682 mL | |
| 80 mM | 0.0290 mL | 0.1452 mL | 0.2904 mL | 0.7261 mL | |
| 100 mM | 0.0232 mL | 0.1162 mL | 0.2324 mL | 0.5809 mL |
Ac4GlcNAz Related Classifications
HY-W039953 Related Classifications
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Keywords
- Ac4GlcNAz
- 98924-81-3
- N-azidoacetylglucosamine-tetraacylated
- Biochemical Assay Reagents
- Helicobacter pylori strain G27
- bacterial cells
- N-acetylglucosamine
- Plesiomonas shigelloides ATCC 51903
- HeLa cells
- cysteine residues
- glycoproteins
- glycans
- Staphylococcus aureus ATCC BAA-1708
- Vibrio vulnificus ATCC 43382
- Inhibitor
- inhibitor
- inhibit