TES sodium
Based on 1 Customer Validation
TES sodium is an excellent hydrogen ion buffer. TES sodium has characteristics such as an appropriate dissociation constant, a low metal-binding constant, and high water solubility. TES sodium can be used in the research of mammalian cell culture, viruses, and so on.
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- Pureté: 98.0%
- CAS No.: 70331-82-7
- Formule: C6H14NNaO6S
- Masse moléculaire:251.23
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
TES (0.1 M) sodium has pKa values of 7.92, 7.50, and 7.14 at 0°, 20° and 37°, respectively, and the concentration of its saturated aqueous solution at 0° is 2.6 M[1].
TES (6-50 mM; 11-14 days) sodium in a closed culture environment is similar to or even more effective than the NaHCO3-CO2-buffered medium in terms of colony-forming efficiency, colony size, and cell morphology of several mammalian cell lines (HeLa S3, KB, BHK 21-Cl3, 3Y1-B Cl-1)[2].
TES (25-50 mM; 14-19 days) sodium has a similar efficiency to NaHCO3-CO2-buffered medium and the required time is shorter in the SV40 virus plaque assay with monkey kidney CV-1 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 70331-82-7
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Appearance Solid
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Masse moléculaire 251.23
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Formule C6H14NNaO6S
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Color White to off-white
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SMILES
OCC(NCCS(=O)(O[Na])=O)(CO)CO
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
H2O : 250 mg/mL (995.10 mM; Need ultrasonic)
DMSO : 83.33 mg/mL (331.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. [Content Brief]
[2]. A Itagaki, et al. Tes and HEPES buffers in mammalian cell cultures and viral studies: problem of carbon dioxide requirement. Exp Cell Res. 1974 Feb;83(2):351-61. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.9804 mL | 19.9021 mL | 39.8042 mL | 99.5104 mL |
| 5 mM | 0.7961 mL | 3.9804 mL | 7.9608 mL | 19.9021 mL | |
| 10 mM | 0.3980 mL | 1.9902 mL | 3.9804 mL | 9.9510 mL | |
| 15 mM | 0.2654 mL | 1.3268 mL | 2.6536 mL | 6.6340 mL | |
| 20 mM | 0.1990 mL | 0.9951 mL | 1.9902 mL | 4.9755 mL | |
| 25 mM | 0.1592 mL | 0.7961 mL | 1.5922 mL | 3.9804 mL | |
| 30 mM | 0.1327 mL | 0.6634 mL | 1.3268 mL | 3.3170 mL | |
| 40 mM | 0.0995 mL | 0.4976 mL | 0.9951 mL | 2.4878 mL | |
| 50 mM | 0.0796 mL | 0.3980 mL | 0.7961 mL | 1.9902 mL | |
| 60 mM | 0.0663 mL | 0.3317 mL | 0.6634 mL | 1.6585 mL | |
| 80 mM | 0.0498 mL | 0.2488 mL | 0.4976 mL | 1.2439 mL | |
| 100 mM | 0.0398 mL | 0.1990 mL | 0.3980 mL | 0.9951 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
TES sodium Related Classifications
HY-23430A Related Classifications
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