Cotrimoxazole (Trimethoprim/sulfamethoxazole 1:19)
Based on 2 publication(s) in Google Scholar
Cotrimoxazole (Trimethoprim/sulfamethoxazole 1:19), an antibacterial agent, is a Trimethoprim (HY-B0510) and Sulfamethoxazole (HY-B0322) mixture. The ratio of Trimethoprim to Sulfamethoxazole in the combination mixture is 1 : 19.
For research use only. We do not sell to patients.
- Purity : 99.83%
- CAS No.: 8064-90-2
- Formula: C24H29N7O6S
- Molecular Weight:543.60
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cotrimoxazole (Trimethoprim/sulfamethoxazole 1:19)
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Customer Validation & Images
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Microbiological Assay
Biological Activity
Description
In Vitro
Cotrimoxazole (Trimethoprim/sulfamethoxazole 1:19) exhibits antibacterial activity against clinical isolates of Escherichia coli, Klebsiella species, Enterobacter species, indole-negative Proteus, indole-positive Proteus, and Serratia marcescens[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Chemical Information
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CAS No. 8064-90-2
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Appearance Solid
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Molecular Weight 543.60
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Formula C24H29N7O6S
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Color White to off-white
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SMILES
O=S(NC1=NOC(C)=C1)(C2=CC=C(C=C2)N)=O.NC3=NC=C(CC4=CC(OC)=C(C(OC)=C4)OC)C(N)=N3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Microorganisms
Antimicrobial Resistance and Genomic Characteristics of Escherichia coli Strains Isolated from the Poultry Industry in Henan Province, China. [Abstract]2024 Mar 13;12(3):575. PMID: 38543626
Cotrimoxazole (Trimethoprim/sulfamethoxazole 1:19) purchased from MedChemExpress. Usage Cited in: Microorganisms. 2024 Mar 13;12(3):575. [Abstract]
Percentages of E. coli strains showing antimicrobial resistance to different antimicrobial agents. AMP, Ampicillin; CAZ, Ceftazidime; IPM, Imipenem; MRP, Meropenem; GEN, Gentamicin; OFX, Ofloxacin; SXT, Trimethoprim–sulfamethoxazole; TET, Tetracycline; TGC, Tigecycline; CL, Colistin; FFC, Florfenicol.
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Microbiol Spectr
Antimicrobial resistance of rapidly growing mycobacteria isolated from companion animals in Taiwan. [Abstract]2025 Jul;13(7):e0307424. PMID: 40387379
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (183.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (617 KB)
- English - EN (617 KB)
- Français - FR (617 KB)
- Deutsch - DE (617 KB)
- Norwegian - NO (617 KB)
- Español - ES (617 KB)
- Swedish - SV (617 KB)
- Italian - IT (617 KB)
- Korean - KR (617 KB)
- Portuguese - PT (617 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8396 mL | 9.1979 mL | 18.3959 mL | 45.9897 mL |
| 5 mM | 0.3679 mL | 1.8396 mL | 3.6792 mL | 9.1979 mL | |
| 10 mM | 0.1840 mL | 0.9198 mL | 1.8396 mL | 4.5990 mL | |
| 15 mM | 0.1226 mL | 0.6132 mL | 1.2264 mL | 3.0660 mL | |
| 20 mM | 0.0920 mL | 0.4599 mL | 0.9198 mL | 2.2995 mL | |
| 25 mM | 0.0736 mL | 0.3679 mL | 0.7358 mL | 1.8396 mL | |
| 30 mM | 0.0613 mL | 0.3066 mL | 0.6132 mL | 1.5330 mL | |
| 40 mM | 0.0460 mL | 0.2299 mL | 0.4599 mL | 1.1497 mL | |
| 50 mM | 0.0368 mL | 0.1840 mL | 0.3679 mL | 0.9198 mL | |
| 60 mM | 0.0307 mL | 0.1533 mL | 0.3066 mL | 0.7665 mL | |
| 80 mM | 0.0230 mL | 0.1150 mL | 0.2299 mL | 0.5749 mL | |
| 100 mM | 0.0184 mL | 0.0920 mL | 0.1840 mL | 0.4599 mL |
Cotrimoxazole (Trimethoprim/sulfamethoxazole 1:19) Related Classifications
HY-141619A Related Classifications
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