Nitrofurantoin

Name: Nitrofurantoin
Brand: MedChemExpress
SKU: HY-A0090
Rating: 5.0 (8 reviews)

Cat. No.: HY-A0090 Purity: 99.95%: Data Sheet
SDS

COA
Handling Instructions
FAQs
Technical Support

Nitrofurantoin is a potent and orally active broad-spectrum beta-lactamase antimicrobial agent. Nitrofurantoin acts as an antibiotic and can be used for the study of urinary tract infections (UTIs), including cystitis and kidney infections.

For research use only. We do not sell to patients.

Nitrofurantoin Chemical Structure

CAS No. : 67-20-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Nitrofurantoin:

8 Publications Citing Use of MCE Nitrofurantoin

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

β-lactam

Cellular Effect

Cell Line	Type	Value	Description	References
5637	IC₅₀	21.3 μM Compound: NF	Cytotoxicity against human 5637 cells after 96 hrs by microtiter assay Cytotoxicity against human 5637 cells after 96 hrs by microtiter assay	[PMID: 18187237]
A-427	IC₅₀	1.86 μM Compound: NF	Cytotoxicity against human A427 cells after 96 hrs by microtiter assay Cytotoxicity against human A427 cells after 96 hrs by microtiter assay	[PMID: 18187237]
DAN-G	IC₅₀	6.74 μM Compound: NF	Cytotoxicity against human DAN-G cells after 96 hrs by microtiter assay Cytotoxicity against human DAN-G cells after 96 hrs by microtiter assay	[PMID: 18187237]
FHC	CC₅₀	> 100 μM Compound: Nft	Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by alamar blue assay Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by alamar blue assay	[PMID: 33007545]
KYSE-510	IC₅₀	29 μM Compound: NF	Cytotoxicity against human KYSE510 cells after 96 hrs by microtiter assay Cytotoxicity against human KYSE510 cells after 96 hrs by microtiter assay	[PMID: 18187237]
KYSE-70 cell line	IC₅₀	22.8 μM Compound: NF	Cytotoxicity against human KYSE70 cells after 96 hrs by microtiter assay Cytotoxicity against human KYSE70 cells after 96 hrs by microtiter assay	[PMID: 18187237]
LCLC-103H cell line	IC₅₀	2.34 μM Compound: NF	Cytotoxicity against human LCLC-103H cells after 96 hrs by microtiter assay Cytotoxicity against human LCLC-103H cells after 96 hrs by microtiter assay	[PMID: 18187237]
MCF7	IC₅₀	4.44 μM Compound: NF	Cytotoxicity against human MCF7 cells after 96 hrs by microtiter assay Cytotoxicity against human MCF7 cells after 96 hrs by microtiter assay	[PMID: 18187237]
RT-4	IC₅₀	7 μM Compound: NF	Cytotoxicity against human RT4 cells after 96 hrs by microtiter assay Cytotoxicity against human RT4 cells after 96 hrs by microtiter assay	[PMID: 18187237]
SISO	IC₅₀	7.27 μM Compound: NF	Cytotoxicity against human SISO cells after 96 hrs by microtiter assay Cytotoxicity against human SISO cells after 96 hrs by microtiter assay	[PMID: 18187237]
Vero	IC₅₀	0.9 nM Compound: Nitrofuradantoin	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
Vero	CC₅₀	23 μg/mL Compound: nitrofurantoin	Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay	[PMID: 22691154]

In Vitro

Nitrofurantoin (0-512 mg/L; 8 h) treatment inhibits the growth of E. coli isolates^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	E. coli isolates: DA10708, DA13815, DA13824, DA13957, DA13992, DA10626, DA10627
Concentration:	0, 32, 64, 128, 256, and 512 mg/L
Incubation Time:	8 hours
Result:	Observed bactericidal effect at 32 mg/L For DA10708, DA13815 and DA13824. Inhibited the growth of DA13957 and DA13992 completely at 128 mg/L, observed bactericidal effect at 256 mg/L. Showed a moderate killing at >128 mg/L for DA10626 and DA10627.

In Vivo

1.19 Nitrofurantoin pharmacokinetic effects in SD Rats^[4].

Parameters	10 mg/kg p.o.	2 mg/kg i.v.
AUC_0-720 (μg/mL·min)	306
AUC_0-120 (μg/mL·min)		90.3
AUC_0-∞ (μg/mL·min)	344	91.5
C_max (μg/mL)	1.01
CL/F or CL (ml/min/kg)	31.0	22.7
t_1/2 (min)	166	23.6
Bioavailability (%)	60.1

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

238.16

Formula

C₈H₆N₄O₅

CAS No.

67-20-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC(CN1/N=C/C2=CC=C([N+]([O-])=O)O2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (419.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1989 mL	20.9943 mL	41.9886 mL
5 mM	0.8398 mL	4.1989 mL	8.3977 mL
10 mM	0.4199 mL	2.0994 mL	4.1989 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (278 KB) SDS (419 KB)

COA (248 KB) HNMR (132 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Huttner A, et al. Nitrofurantoin revisited: a systematic review and meta-analysis of controlled trials. J Antimicrob Chemother. 2015 Sep;70(9):2456-64. [Content Brief]

[2]. Garau J. Other antimicrobials of interest in the era of extended-spectrum beta-lactamases: fosfomycin, nitrofurantoin and tigecycline. Clin Microbiol Infect. 2008 Jan;14 Suppl 1:198-202. [Content Brief]

[3]. Linus Sandegren, et al. Nitrofurantoin resistance mechanism and fitness cost in Escherichia coli. J Antimicrob Chemother. 2008 Sep;62(3):495-503. [Content Brief]

[4]. Xiaodong Wang, et al. Effects of the flavonoid chrysin on nitrofurantoin pharmacokinetics in rats: potential involvement of ABCG2. Drug Metab Dispos. 2007 Feb;35(2):268-74. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1989 mL	20.9943 mL	41.9886 mL	104.9714 mL
	5 mM	0.8398 mL	4.1989 mL	8.3977 mL	20.9943 mL
	10 mM	0.4199 mL	2.0994 mL	4.1989 mL	10.4971 mL
	15 mM	0.2799 mL	1.3996 mL	2.7992 mL	6.9981 mL
	20 mM	0.2099 mL	1.0497 mL	2.0994 mL	5.2486 mL
	25 mM	0.1680 mL	0.8398 mL	1.6795 mL	4.1989 mL
	30 mM	0.1400 mL	0.6998 mL	1.3996 mL	3.4990 mL
	40 mM	0.1050 mL	0.5249 mL	1.0497 mL	2.6243 mL
	50 mM	0.0840 mL	0.4199 mL	0.8398 mL	2.0994 mL
	60 mM	0.0700 mL	0.3499 mL	0.6998 mL	1.7495 mL
	80 mM	0.0525 mL	0.2624 mL	0.5249 mL	1.3121 mL
	100 mM	0.0420 mL	0.2099 mL	0.4199 mL	1.0497 mL

Nitrofurantoin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitrofurantoin67-20-9BacterialAntibioticantimicrobialantibioticurinary tractinfectionUTIcystitiskidneyE. coliInhibitorinhibitorinhibit

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Nitrofurantoin

Customer Review

Other Forms of Nitrofurantoin:

Nitrofurantoin Related Antibodies

8 Publications Citing Use of MCE Nitrofurantoin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Nitrofurantoin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Nitrofurantoin Related Classifications

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