Nitrofurantoin
Based on 10 publication(s) in Google Scholar
Nitrofurantoin is a potent and orally active broad-spectrum beta-lactamase antimicrobial agent. Nitrofurantoin acts as an antibiotic and can be used for the study of urinary tract infections (UTIs), including cystitis and kidney infections.
For research use only. We do not sell to patients.
- Purity: 99.71%
- CAS No.: 67-20-9
- Formula: C8H6N4O5
- Molecular Weight:238.16
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Anti-Infection Compound Library
HY-L002
-
Immunology/Inflammation Compound Library
HY-L007
-
FDA-Approved Drug Library
HY-L022
-
Drug Repurposing Compound Library
HY-L035
-
Covalent Screening Library
HY-L036
-
Antibacterial Compound Library
HY-L049
-
NMPA-Approved Drug Library
HY-L053
-
Orally Active Compound Library
HY-L061
-
FDA Approved & Pharmacopeial Drug Library
HY-L066
-
Antibiotics Library
HY-L067
-
Drug-Induced Liver Injury (DILI) Compound Library
HY-L076
-
Rare Diseases Drug Library
HY-L102
-
Pediatric Drug Library
HY-L104
-
Human Metabolite Library
HY-L123
-
Anti-Prostate Cancer Compound Library
HY-L124
-
Heterocyclic Compound Library
HY-L138
-
Off-patent Drug Library
HY-L141
-
Mitochondrial Toxicity Compound Library
HY-L155
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Covalent Screening Library Plus
HY-L036P
-
Drug Repurposing Compound Library Plus
HY-L035P
-
FDA-Approved Drug Library Plus
HY-L022P
-
FDA-Approved Drug Library Mini
HY-L022M
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
-
Mass Spectrometry Human Metabolite Library
HY-L215
Publications Citing Use of MedChemExpress (MCE) Nitrofurantoin
More- Nat Commun. 2022 Mar 2;13(1):1116. [Abstract]
- Water Res. 2023 Jul 15:240:120110. [Abstract]
- J Exp Med. 2026 Mar 2;223(3):e20241287. [Abstract]
- Microbiol Spectr. 2022 Feb 23;10(1):e0099121. [Abstract]
- Biotechnol Bioeng. 2021 Dec;118(12):4687-4698. [Abstract]
- Diagn Microbiol Infect Dis. 2026 Feb;114(2):117181. [Abstract]
- bioRxiv. 2025 January 19.
- bioRxiv. 2024 May 10.
- Research Square Preprint. 2021 Aug.
- Research Square Preprint. 2020 Jun.
All Antibiotic Isoforms
More
Biological Activity
|
β-lactam |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 5637 | IC50 |
21.3 μM
Compound: NF
|
Cytotoxicity against human 5637 cells after 96 hrs by microtiter assay
Cytotoxicity against human 5637 cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| A-427 | IC50 |
1.86 μM
Compound: NF
|
Cytotoxicity against human A427 cells after 96 hrs by microtiter assay
Cytotoxicity against human A427 cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| Caco-2 | IC50 |
0.115 μM
Compound: NF
|
Anticancer activity against human Caco-2 cells incubated for 72 hrs by MTT assay
Anticancer activity against human Caco-2 cells incubated for 72 hrs by MTT assay
|
[PMID: 36921275] |
| DAN-G | IC50 |
6.74 μM
Compound: NF
|
Cytotoxicity against human DAN-G cells after 96 hrs by microtiter assay
Cytotoxicity against human DAN-G cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| FHC | CC50 |
>100 μM
Compound: Nft
|
Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by alamar blue assay
Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by alamar blue assay
|
[PMID: 33007545] |
| HeLa | IC50 |
0.166 μM
Compound: NF
|
Anticancer activity against human HeLa cells incubated for 72 hrs by MTT assay
Anticancer activity against human HeLa cells incubated for 72 hrs by MTT assay
|
[PMID: 36921275] |
| KYSE-510 | IC50 |
29 μM
Compound: NF
|
Cytotoxicity against human KYSE510 cells after 96 hrs by microtiter assay
Cytotoxicity against human KYSE510 cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| KYSE-70 cell line | IC50 |
22.8 μM
Compound: NF
|
Cytotoxicity against human KYSE70 cells after 96 hrs by microtiter assay
Cytotoxicity against human KYSE70 cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| LCLC-103H cell line | IC50 |
2.34 μM
Compound: NF
|
Cytotoxicity against human LCLC-103H cells after 96 hrs by microtiter assay
Cytotoxicity against human LCLC-103H cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| MCF7 | IC50 |
4.44 μM
Compound: NF
|
Cytotoxicity against human MCF7 cells after 96 hrs by microtiter assay
Cytotoxicity against human MCF7 cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| RT-4 | IC50 |
7 μM
Compound: NF
|
Cytotoxicity against human RT4 cells after 96 hrs by microtiter assay
Cytotoxicity against human RT4 cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| Sf21 | IC50 |
>1000 μM
Compound: Nitrofurantoin
|
Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
|
[PMID: 21965623] |
| Sf21 | IC50 |
>1000 μM
Compound: Nitrofurantoin
|
Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
|
[PMID: 21965623] |
| SISO | IC50 |
7.27 μM
Compound: NF
|
Cytotoxicity against human SISO cells after 96 hrs by microtiter assay
Cytotoxicity against human SISO cells after 96 hrs by microtiter assay
|
[PMID: 18187237] |
| THP-1 | IC50 |
>100 μM
Compound: NFT
|
Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
|
[PMID: 36516584] |
| Vero | IC50 |
0.9 nM
Compound: Nitrofuradantoin
|
Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay
Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay
|
[PMID: 10464017] |
| Vero | CC50 |
23 μg/mL
Compound: nitrofurantoin
|
Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay
Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay
|
[PMID: 22691154] |
| Vero | IC50 |
>100 μM
Compound: NFT
|
Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
|
[PMID: 36516584] |
| WI-38 | IC50 |
0.162 μM
Compound: NF
|
Cytotoxicity in human WI-38 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity in human WI-38 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 36921275] |
Nitrofurantoin (0-512 mg/L; 8 h) treatment inhibits the growth of E. coli isolates[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:E. coli isolates: DA10708, DA13815, DA13824, DA13957, DA13992, DA10626, DA10627
-
Concentration:0, 32, 64, 128, 256, and 512 mg/L
-
Incubation Time:8 hours
-
Result:Observed bactericidal effect at 32 mg/L For DA10708, DA13815 and DA13824.
Inhibited the growth of DA13957 and DA13992 completely at 128 mg/L, observed bactericidal effect at 256 mg/L.
Showed a moderate killing at >128 mg/L for DA10626 and DA10627.
| Parameters | 10 mg/kg p.o. | 2 mg/kg i.v. |
| AUC0-720 (μg/mL·min) | 306 | |
| AUC0-120 (μg/mL·min) | 90.3 | |
| AUC0-∞ (μg/mL·min) | 344 | 91.5 |
| Cmax (μg/mL) | 1.01 | |
| CL/F or CL (ml/min/kg) | 31.0 | 22.7 |
| t1/2 (min) | 166 | 23.6 |
| Bioavailability (%) | 60.1 | |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
CAS No. 67-20-9
-
Appearance Solid
-
Molecular Weight 238.16
-
Formula C8H6N4O5
-
Color Light yellow to yellow
-
SMILES
O=C1NC(CN1/N=C/C2=CC=C([N+]([O-])=O)O2)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (10)
-
Journal Impact Factor
-
Most Recent
-
Nat Commun
Antimicrobial resistance and population genomics of multidrug-resistant Escherichia coli in pig farms in mainland China. [Abstract]2022 Mar 2;13(1):1116. PMID: 35236849 -
Water Res
Quantifying community-wide antibiotic usage via urban water fingerprinting: Focus on contrasting resource settings in South Africa. [Abstract]2023 Jul 15:240:120110. PMID: 37247434 -
J Exp Med
2026 Mar 2;223(3):e20241287. PMID: 41400657 -
Microbiol Spectr
The Mechanism of Action of Ginkgolic Acid (15:1) against Gram-Positive Bacteria Involves Cross Talk with Iron Homeostasis. [Abstract]2022 Feb 23;10(1):e0099121. PMID: 35019708 -
Biotechnol Bioeng
An integrated biomimetic array chip for establishment of collagen-based 3D primary human hepatocyte model for prediction of clinical drug-induced liver injury. [Abstract]2021 Dec;118(12):4687-4698. PMID: 34478150 -
Diagn Microbiol Infect Dis
2026 Feb;114(2):117181. PMID: 41205476 -
-
-
-
Solvent & Solubility
DMSO : 100 mg/mL (419.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
-
Handling Instructions (2659 KB)
References
[1]. Huttner A, et al. Nitrofurantoin revisited: a systematic review and meta-analysis of controlled trials. J Antimicrob Chemother. 2015 Sep;70(9):2456-64. [Content Brief]
[2]. Garau J. Other antimicrobials of interest in the era of extended-spectrum beta-lactamases: fosfomycin, nitrofurantoin and tigecycline. Clin Microbiol Infect. 2008 Jan;14 Suppl 1:198-202. [Content Brief]
[3]. Linus Sandegren, et al. Nitrofurantoin resistance mechanism and fitness cost in Escherichia coli. J Antimicrob Chemother. 2008 Sep;62(3):495-503. [Content Brief]
[4]. Xiaodong Wang, et al. Effects of the flavonoid chrysin on nitrofurantoin pharmacokinetics in rats: potential involvement of ABCG2. Drug Metab Dispos. 2007 Feb;35(2):268-74. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1989 mL | 20.9943 mL | 41.9886 mL | 104.9714 mL |
| 5 mM | 0.8398 mL | 4.1989 mL | 8.3977 mL | 20.9943 mL | |
| 10 mM | 0.4199 mL | 2.0994 mL | 4.1989 mL | 10.4971 mL | |
| 15 mM | 0.2799 mL | 1.3996 mL | 2.7992 mL | 6.9981 mL | |
| 20 mM | 0.2099 mL | 1.0497 mL | 2.0994 mL | 5.2486 mL | |
| 25 mM | 0.1680 mL | 0.8398 mL | 1.6795 mL | 4.1989 mL | |
| 30 mM | 0.1400 mL | 0.6998 mL | 1.3996 mL | 3.4990 mL | |
| 40 mM | 0.1050 mL | 0.5249 mL | 1.0497 mL | 2.6243 mL | |
| 50 mM | 0.0840 mL | 0.4199 mL | 0.8398 mL | 2.0994 mL | |
| 60 mM | 0.0700 mL | 0.3499 mL | 0.6998 mL | 1.7495 mL | |
| 80 mM | 0.0525 mL | 0.2624 mL | 0.5249 mL | 1.3121 mL | |
| 100 mM | 0.0420 mL | 0.2099 mL | 0.4199 mL | 1.0497 mL |
Powered by Bioz