Cy5 DBCO chloride 

Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free "Click Chemistry" reaction^[1]. Cy5 DBCO (chloride) is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

Cy5 DBCO chloride (2-50 μM) labels cells in a dose-dependent manner, and the amount of DBCO-Cy5 bound to the cell surface increases with increasing DBCO-Cy5 concentration^[1].
Guide (The following is a recommended protocol for reference only and should be adjusted according to your specific experimental needs).
1. Seed the cells in a culture dish and culture for 3 days, then wash the cells twice with DPBS (pH 7.4).
2. Add Cy5 DBCO chloride at a final concentration of 20 μM and incubate at 37℃ for 1 h.
3. After incubation, wash the cells with DPBS (pH 7.4), then fix them with formaldehyde-glutaraldehyde combined fixative at room temperature for 15 min.
4. After fixation, wash the cells twice with DPBS (pH 7.4), and analyze the samples using a flow cytometer, fluorescence microscope, or fluorescence microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

777.43

C₅₀H₅₃ClN₄O₂

2182601-72-3

668

647

CC1(C)C(/C=C/C=C/C=C2N(CCCCCC(NCCC(N3C4=CC=CC=C4C#CC5=CC=CC=C5C3)=O)=O)C6=C(C=CC=C6)C/2(C)C)=[N+](C)C7=C1C=CC=C7.[Cl-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sun-Woong Kang, et al. Cell labeling and tracking method without distorted signals by phagocytosis of macrophages. Theranostics. 2014 Feb 12;4(4):420-31.  [Content Brief]