Cy5 DBCO chloride
Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free "Click Chemistry" reaction. Cy5 DBCO (chloride) is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- CAS No.: 2182601-72-3
- Formula: C50H53ClN4O2
- Molecular Weight:777.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Cy5 DBCO chloride (2-50 μM) labels cells in a dose-dependent manner, and the amount of DBCO-Cy5 bound to the cell surface increases with increasing DBCO-Cy5 concentration[1].
Guide (The following is a recommended protocol for reference only and should be adjusted according to your specific experimental needs).
1. Seed the cells in a culture dish and culture for 3 days, then wash the cells twice with DPBS (pH 7.4).
2. Add Cy5 DBCO chloride at a final concentration of 20 μM and incubate at 37℃ for 1 h.
3. After incubation, wash the cells with DPBS (pH 7.4), then fix them with formaldehyde-glutaraldehyde combined fixative at room temperature for 15 min.
4. After fixation, wash the cells twice with DPBS (pH 7.4), and analyze the samples using a flow cytometer, fluorescence microscope, or fluorescence microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
668
Excitation (Ex)
647
Chemical Information
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CAS No. 2182601-72-3
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Molecular Weight 777.43
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Formula C50H53ClN4O2
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SMILES
CC1(C)C(/C=C/C=C/C=C2N(CCCCCC(NCCC(N3C4=CC=CC=C4C#CC5=CC=CC=C5C3)=O)=O)C6=C(C=CC=C6)C/2(C)C)=[N+](C)C7=C1C=CC=C7.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cy5 DBCO chloride Related Classifications
HY-D1625 Related Classifications
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