Cy5 DBCO chloride
Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free "Click Chemistry" reaction. Cy5 DBCO (chloride) is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- CAS No.: 2182601-72-3
- Formula: C50H53ClN4O2
- Molecular Weight:777.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cy5 DBCO chloride (2-50 μM) labels cells in a dose-dependent manner, and the amount of DBCO-Cy5 bound to the cell surface increases with increasing DBCO-Cy5 concentration[1].
Guide (The following is a recommended protocol for reference only and should be adjusted according to your specific experimental needs).
1. Seed the cells in a culture dish and culture for 3 days, then wash the cells twice with DPBS (pH 7.4).
2. Add Cy5 DBCO chloride at a final concentration of 20 μM and incubate at 37℃ for 1 h.
3. After incubation, wash the cells with DPBS (pH 7.4), then fix them with formaldehyde-glutaraldehyde combined fixative at room temperature for 15 min.
4. After fixation, wash the cells twice with DPBS (pH 7.4), and analyze the samples using a flow cytometer, fluorescence microscope, or fluorescence microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 2182601-72-3
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Molecular Weight 777.43
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Formula C50H53ClN4O2
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SMILES
CC1(C)C(/C=C/C=C/C=C2N(CCCCCC(NCCC(N3C4=CC=CC=C4C#CC5=CC=CC=C5C3)=O)=O)C6=C(C=CC=C6)C/2(C)C)=[N+](C)C7=C1C=CC=C7.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cy5 DBCO chloride Related Classifications
HY-D1625 Related Classifications
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